Search results for "RICH"
showing 10 items of 3360 documents
Effect of mismatch repair on the mutation rate of bacteriophage ϕX174
2015
Viral mutation rates vary widely in nature, yet the mechanistic and evolutionary determinants of this variability remain unclear. Small DNA viruses mutate orders of magnitude faster than their hosts despite using host-encoded polymerases for replication, which suggests these viruses may avoid post-replicative repair. Supporting this, the genome of bacteriophage ϕX174 is completely devoid of GATC sequence motifs, which are required for methyl-directed mismatch repair in Escherichia coli . Here, we show that restoration of the randomly expected number of GATC sites leads to an eightfold reduction in the rate of spontaneous mutation of the phage, without severely impairing its replicative capa…
New insight into the haemoglobin superfamily: preliminary crystallographic characterization of human cytoglobin.
2003
Human cytoglobin, present in almost all tissue types, is a newly identified member of the Hb superfamily. A double mutant, having both cysteines replaced by serines, has been overexpressed in Escherichia coli, purified and crystallized. A highly redundant SAD data set has been collected at the haem Fe-atom absorption edge (lambda = 1.720 A) to 2.60 A resolution. The crystals belong to the orthorhombic P2(1)2(1)2(1) space group, with unit-cell parameters a = 46.8, b = 73.1, c = 98.9 A and two molecules per asymmetric unit. The anomalous difference Patterson map clearly reveals the position of the haem Fe-atom sites, thus paving the way for SAD structure determination.
Novel mutations of the ABCA12, KRT1 and ST14 genes in three unrelated newborns showing congenital ichthyosis.
2022
Abstract Background Congenital ichthyosis (CI) is a heterogeneous group of genetic disorders characterized by generalized dry skin, scaling and hyperkeratosis, often associated to erythroderma. They are rare diseases, with overall incidence of 6.7 in 100,000. Clinical manifestations are due to mutations in genes mostly involved in skin barrier formation. Based on clinical presentation, CI is distinguished in non-syndromic and syndromic forms. To date, mutations of more than 50 genes have been associated to different types of CI. Cases presentation We report on three Italian unrelated newborns showing clinical signs compatible with different forms of CI of variable severity, namely Harlequin…
Polyphasic identification of yeasts isolated from bark of cork oak during the manufacturing process of cork stoppers.
2003
A two-step protocol was used for the identification of 52 yeasts isolated from bark of cork oak at initial stages of the manufacturing process of cork stoppers. The first step in the identification was the separation of the isolates into groups by their physiological properties and RFLPs of the ITS-5.8S rRNA gene. The second step was the sequencing of the D1/D2 domains of the 26S rRNA gene of selected isolates representing the different groups. The results revealed a predominance of basidiomycetous yeasts (11 species), while only two species represented the ascomycetous yeasts. Among the basidiomycetous yeasts, members representing the species Rhodosporidium kratochvilovae and Rhodotorula n…
Summer aggregation of common eagle ray, Myliobatis aquila (Chondrichthyes: Myliobatidae), in the Marine Protected Area of the Egadi Islands (southwes…
2021
Author(s): Grancagnolo, Desiree; Arculeo, Marco | Abstract: We report for the first time in Italian waters a periodic and frequent presence of a summer aggregation of common eagle ray Myliobatis aquila in the island of Marettimo (southwestern Tyrrhenian Sea), which is part of the Marine Protected Area of the Egadi Islands. These aggregation could be related to different phenomena such as reproduction, feeding or simply as a transit zone.
C-terminal amino acids are essential for human heat shock protein 70 dimerization
2014
The human inducible heat shock protein 70 (hHsp70), which is involved in several major pathologies, including neurodegenerative disorders and cancer, is a key molecular chaperone and contributes to the proper protein folding and maintenance of a large number of protein structures. Despite its role in disease, the current structural knowledge of hHsp70 is almost exclusively based on its Escherichia coli homolog, DnaK, even though these two proteins only share ~50 % amino acid identity. For the first time, we describe a complete heterologous production and purification strategy that allowed us to obtain a large amount of soluble, full-length, and non-tagged hHsp70. The protein displayed both …
The role of landscape, topography, and geodiversity in explaining vascular plant species richness in a fragmented landscape
2016
We explained vascular plant species richness patterns in a 286 km(2) fragmented landscape with a notable human influence. The objective of this study was two-fold: to test the relative importance of landscape, topography and geodiversity measures, and to compare three different landscape-type variables in species richness modeling. Moreover, we tested if results differ when only native species are considered. We used generalized linear modeling based variation partitioning and generalized additive models with different explanatory variable sets. Landscape and topography explained the majority of the variation but the relative importance of topography and geodiversity was higher in explainin…
Single-neutron orbits near Ni-78: Spectroscopy of the N=49 isotope Zn-79
2015
5 pags., 6 figs.
Memory cell structure integrated on semiconductor
2004
This invention relates to a memory cell Which comprises a capacitor having a ?rst electrode and a second electrode separated by a dielectric layer. Such dielectric layer com prises a layer of a semi-insulating material Which is fully enveloped by an insulating material and in Which an electric charge is permanently present or trapped therein. Such electric charge accumulated close to the ?rst or to the second electrode, depending on the electric ?eld betWeen the electrodes,therebyde?ningdifferentlogiclevels.
Meiobenthic naidid and aeolosomatid oligochaetes from the profundal zone, and relations of species to eutrophication
1989
Oligochaetes were sampled using meiobenthos methods from depths of between 20 and 94 m in Lake Paijanne in 1986. Individuals belonging to several species of the Naididae and Aeolosomatidae, which are generally considered to include mainly littoral or lotic species, were found more or less regularly at these profundal depths. The naidid species Chaetogaster langi and Arnphichaeta leydigii were more abundant in the deepest areas than at 20 m. Species also appeared to react differently to water quality. The naidid species Chaetogaster langi and the aeolosomatid species Aeolosoma quaternarium, A. hernprichi and Rheomorpha neiswestnovae were most abundant on average at oligotrophic, unpolluted s…