Search results for "RICH"
showing 10 items of 3360 documents
Dithiodiketopiperazine derivatives from endophytic fungi Trichoderma harzianum and Epicoccum nigrum
2021
A new epidithiodiketopiperazine (ETP), pretrichodermamide G (1), along with three known (epi)dithiodiketopiparazines (2-4) were isolated from cultures of Trichoderma harzianum and Epicoccum nigrum, endophytic fungi associated with medicinal plants Zingiber officinale and Salix sp., respectively. The structure of the new compound (1) was established on the basis of spectroscopic data, including 1D/2D NMR and HRESIMS. The isolated compounds were investigated for their antifungal, antibacterial and cytotoxic potential against a panel of microorganisms and cell lines. Pretrichodermamide A (2) displayed antimicrobial activity towards the plant pathogenic fungus Ustilago maydis and the human path…
A peculiar new Orthotrichum species (Orthotrichaceae, Bryopsida) from central Argentina
2007
A new epiphytic Orthotrichum species (Orthotrichum spiculatum F. Lara, Garilleti & Mazimpaka sp. nov.) is described from the sierra of Cordoba (Argentina). The new species has most of the characteristics of subgenus Pulchella (Schimp.) Vitt, but it is unique because of having eight exostome teeth pairs and 16 broad, strongly papillose endostome segments. Additionally, its upper leaves have green, acuminate, dentate–spiculate apices. © 2007 The Linnean Society of London, Botanical Journal of the Linnean Society, 2007, 155, 477–482.
The L-tartrate/succinate antiporter TtdT (YgjE) of L-tartrate fermentation in Escherichia coli.
2007
ABSTRACT Escherichia coli ferments l -tartrate under anaerobic conditions in the presence of an additional electron donor to succinate. The carrier for l -tartrate uptake and succinate export and its relation to the general C 4 -dicarboxylate carriers DcuA, DcuB, and DcuC were studied. The secondary carrier TtdT, encoded by the ttdT (previously called ygjE ) gene, is required for the uptake of l -tartrate. The ttdT gene is located downstream of the ttdA and ttdB genes, encoding the l -tartrate dehydratase TtdAB. Analysis of mRNA by reverse transcription-PCR showed that ttdA , ttdB , and ttdT are cotranscribed. Deletion of ttdT abolished growth by l -tartrate and degradation of l -tartrate c…
Utilizing genetically engineered bacteria to produce plant-specific glucosides
2001
Plant-derived glucosides have attracted much attention due to their widespread applications. This class of products is difficult to isolate or to synthesize in pure form because of the resulting low yields. Thus, simple approaches for the generation of such glucosides would be highly beneficial. We purified and characterized a novel glucosyltransferase from plant cell suspension cultures of Rauvolfia serpentina, which showed rather low substrate specificity. We obtained its cDNA and expressed the active recombinant protein in bacteria (Escherichia coli) with excellent plant-specific glucosylation efficiencies. Compared with the plant system, the bacteria delivered the new enzyme, which was …
Antimykotische Wirkstoffe, Mitt. Chlor-(1-piperazinyl)-1,3,5-triazine
1988
Aus der Umsetzung von Cyanurchlorid (1) mit den Piperazinderivaten 2a–c bei –20 bis 10° gehen die Dichlor-(1-piperazinyl)-1,3,5-triazine 3a–c hervor. Bei der erhohten Reaktionstemperatur von 40° erfolgt aus 1 und den Piperazinderivaten 4a–b Bildung der 2-Chlor-4,6-bis(piperazinyl)-1,3,5-triazine 5a–b. Zu gemischt substituierten Chlor-1,3,5-triazinen 9 fuhrt die nacheinander erfolgende Umsetzung von 1 mit 2 verschiedenen cyclischen sekundaren Aminen (6 und 8). Die Strukturtypen 3, 5 und 9 zeigen in den 1H-NMR-Spektren zwei fur Piperazinringe charakteristische Signalgruppen im Bereich 2.4–4.0 ppm. Unter den neu entwickelten Verbindungen finden sich Vertreter mit sehr starker antimykotischer W…
Species diversity of the deep-water gulper sharks (Squaliformes: Centrophoridae:Centrophorus) in North Atlantic waters - current status and taxonomic…
2014
The gulper sharks (genus Centrophorus) are a group of deep-water benthopelagic sharks with a worldwide dis- tribution. The alpha taxonomy of the group has historically been problematic and the number of species included in the genus has varied considerably over the years and is still under debate. Gulper sharks are routinely caught in mid- and deep-water fisheries worldwide and some have shown a considerable decline in abundance in the last few decades. Clear and consistent species discrimination of Centrophorus is essential for an efficient and sustain- able management of these fisheries resources. Our study used molecular cytochrome oxidase subunit I (COI) and 16S ribosomal RNA gene seque…
Real time analysis of Escherichia coli biofilms using calorimetry
2008
Abstract Microbial communities grow more stably when they are associated to surfaces or organized in aggregates. This advantage of biofilms is technically exploited for the degradation of xenobiotics or in biocatalysis, where the fixed biomass has the added advantage of easier separation of excreted products. Whereas the biothermodynamic analysis of growth and product formation of suspended cells is developing fast, there are only few reports on the calorimetric analysis and biothermodynamic interpretation of biofilm evolution. Experiments illustrate the ability of calorimetry to monitor the physiological state of biofilms in real time. Sessile cells of Escherichia coli DH5α DSM 6897 were s…
Transmission Electron Microscopy of GroEL, GroES, and the Symmetrical GroEL/ES Complex
1994
Two new 2-D crystal forms of the Escherichia coli chaperone GroEL (cpn60) 2 x 7-mer have been produced using the negative staining-carbon film (NS-CF) technique. These 2-D crystals, which contain the cylindrical GroEL in side-on and end-on orientations, both possess p21 symmetry, with two molecules in the respective unit cells. The crystallographically averaged images correlate well with those obtained by other authors from single particle analysis of GroEL and our own previous crystallographic analysis. 2-D crystallization of the smaller chaperone GroES (cpn10) 7-mer has also been achieved using the NS-CF technique. Crystallographically averaged images of GroES single particle images indic…
ChemInform Abstract: One-Step Synthesis, Crystallographic Studies and Antimicrobial Activity of New 4-Diazopyrazole Derivatives.
2010
Summary A number of new 4-diazopyrazole derivatives were prepared by the reaction of 1- R -3-methyl-5(R 1 -substituted)benzamidopyrazoles with a sevenfold excess of nitrous acid in acetic medium. The compounds were tested for activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus faecalis, Listeria monocytogenes, Candida albicans, Candida tropicalis and Paecilomyces varioti . The highest microbial susceptibility was shown by Gram-positive bacteria, with minimum inhibitory concentrations (MIC) in the range 0.5–12.5 μg/mL. For S aureus the R 1 substituents were screened utilizing the Topliss operational scheme. The 4-nitro g…
Sporotrichoid Cases of Mycobacterium Marinum Skin Infection.
2010
Mycobacterium marinum belongs to the slow growth photochromogenous mycobacteria group. It is pathogenic for fish and human beings in which it can cause cutaneous nodular and ulcerative lesions, sometimes with sporotrichoid arrangement. We report three cases of sporotrichoid Mycobacterium marinum infections successfully treated with prolonged antibiotic therapy. Tropical fish aquaria and, more in general, the water environment were identified as the source of infection.