Search results for "RNA Precursors"

showing 3 items of 13 documents

Anything but Ordinary - Emerging Splicing Mechanisms in Eukaryotic Gene Regulation.

2021

Splicing of precursor mRNAs (pre-mRNA) is an important step during eukaryotic gene expression. The identification of the actual splice sites and the proper removal of introns are essential for the production of the desired mRNA isoforms and their encoded proteins. While the basic mechanisms of splicing regulation are well understood, recent work has uncovered a growing number of noncanonical splicing mechanisms that play key roles in the regulation of gene expression. In this review, we summarize the current principles of splicing regulation, including the impact of cis and trans regulatory elements, as well as the influence of chromatin structure, transcription, and RNA modifications. We f…

Regulation of gene expression0303 health sciencesRNA SplicingIntronRNAEukaryotaRNA-binding proteinComputational biologyBiologyIntronsChromatin03 medical and health sciencesAlternative Splicing0302 clinical medicineCircular RNARNA splicingGene expressionGeneticsRNA PrecursorsHumansRNA Messenger030217 neurology & neurosurgery030304 developmental biologyTrends in genetics : TIG
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Sequence analysis of the rDNA spacer of Paracentrotus lividus and observations about pre-rRNA processing. NTS sequence of Paracentrotus lividus rDNA.

1993

We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchin Paracentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor while has the same 5' terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.

Sequence analysisMolecular Sequence DataRestriction MappingDNA RibosomalParacentrotus lividusIntergenic regionSpecies SpecificitySequence Homology Nucleic AcidGeneticsRNA PrecursorsAnimalsRNA Processing Post-TranscriptionalRRNA processingMolecular BiologyRibosomal DNAbiologyBase SequenceGeneral MedicineSpacer DNARibosomal RNAbiology.organism_classificationMolecular biologyExternal transcribed spacerSea UrchinsOocytesFemaleMolecular biology reports
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Aberrant splicing of the Drosophila melanogaster phenylalanine hydroxylase pre-mRNA caused by the insertion of a B104/roo transposable element in the…

1999

Abstract We report the insertion of the transposable element B104 in the Phenylalanine hydroxylase gene of the Drosophila mutant Henna-recessive 3 . Its presence alters the Phenylalanine hydroxylase splicing pattern, producing at least two aberrant mRNAs which contain part of the B104 sequence interrupting the coding region. This aberrant splicing is provoked by the use of a cryptic donor site encoded by the B104 3′ long terminal repeat in combination with either the gene intron 3 acceptor site or a novel acceptor site generated by the target duplication caused by transposition. One of them, referred as mRNA type 1, encodes a truncated protein that could be predictably non-functional. In mR…

Transposable elementDNA ComplementaryPhenylalanine hydroxylaseMolecular Sequence DataGenes InsectBiologyBiochemistryRNA PrecursorsAnimalsCoding regionAmino Acid SequenceMolecular BiologyGeneBase SequenceIntronPhenylalanine HydroxylaseExonsTryptophan hydroxylaseMolecular biologyAlternative SplicingMutagenesis InsertionalDrosophila melanogasterInsect ScienceRNA splicingDNA Transposable Elementsbiology.proteinPrecursor mRNAInsect Biochemistry and Molecular Biology
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