Search results for "RNAs"

Plazmas miRNS kā multiplās sklerozes biomarķieri

2018

Vairākos pētījumos ir atklātas miRNS, tajā skaitā cirkulējošas miRNS, kuru daudzums ir izmainīts multiplā sklerozē. miRNS kā jaunu biomarķieru izpēte šai slimībai varētu palīdzēt diagnosticēt un prognozēt tās attīstību agrīnā stadijā. Šajā pētījumā ir prezentēti mūsu dati par dažām miRNS, kas tikadetektētas MS pacientu asins plazmā, kā izmainītas. Mēs salīdzinājām miRNS ekspresijas līmeņus MS un tās apakštipos ar veselo kontroli, lai atrastu potenciālās miRNS, kas var tikt izmantotas, kā MS biomarķieri. Galvenais mērķis bija validēt sekvenēšanas rezultātā atlasītās miRNS, kuru daudzums bija izmainīts MS pacientu asins plazmā neatkarīgā pacientu kopā. miRNS 484, kuras daudzums bija visvairāk…

Identification of Stress Associated microRNAs in

2019

Tomato (Solanum lycopersicum) is one of the most important crops around the world and also a model plant to study response to stress. High-throughput sequencing was used to analyse the microRNA (miRNA) profile of tomato plants undergoing five biotic and abiotic stress conditions (drought, heat, P. syringae infection, B. cinerea infection, and herbivore insect attack with Leptinotarsa decemlineata larvae) and one chemical treatment with a plant defence inducer, hexanoic acid. We identified 104 conserved miRNAs belonging to 37 families and we predicted 61 novel tomato miRNAs. Among those 165 miRNAs, 41 were stress-responsive. Reverse transcription quantitative PCR (RT-qPCR) was used to valida…

Resnās zarnas vēzis: Agrīnie ķirurģiskās ārstēšanas rezultāti Latvijas Onkoloģijas centrā

2020

Kolorektālais vēzis ir viena no visbiežāk sastopamajām onkoloģiskajām saslimšanām pasaulē, kas aizņem trešo vietu pēc plaušu un krūšu vēžiem. Ārstēšanas izvēle ir atkarīga no audzēja veida, stadijas, kā arī no pacienta vispārējā stāvokļa. Ķirurģiska iejaukšanās ir pamata ārstēšana metode pie šīs slimības un diemžēl operācijas gaita un pēcoperācijas periods ar tai sekojošām komplikācijām bieži vien kļūst par pacienta nāves iemeslu.. (Bray et al., 2018) Darba mērķis: Noskaidrot demografisko un intraoperatīvo faktoru ietekmi uz agrīnu pēc operācijas periodu. Materiāli un metodes: Šajā retrospektīvaja pētījumā ietvaros tika analizēti dati par 62 pacientiem ar resnās zarnas vēža diagnozi kuriem …

A perspective analysis: microRNAs, glucose metabolism, and drug resistance in colon cancer stem cells

2021

Metabolism sustains the stemness of Cancer Stem Cells (CSCs), affecting, in turn, tumor heterogeneity, metastatic potential, and therapy resistance. Therefore, it is appealing to target CSCs metabolism as a new therapeutic approach. Consequently, we paid considerable attention to the anti-apoptotic microRNA miR-483-3p, that we reported being regulated by glucose metabolism in liver cancer cells. We investigated the therapeutic potential of targeting miR-483-3p by using the anti-glucose metabolism 2-deoxyglucose (2-DG) molecule in tumor Xenograft mouse model originating from two different Colon-Cancer Stem Cell lines (CCSC lines). We show that 2-DG treatment does not affect CCSCs during tumo…

MiR-221 promotes stemness of breast cancer cells by targeting DNMT3b

2016

// Giuseppina Roscigno 1, 2 , Cristina Quintavalle 1, 2 , Elvira Donnarumma 3 , Ilaria Puoti 1 , Angel Diaz-Lagares 4 , Margherita Iaboni 1 , Danilo Fiore 1 , Valentina Russo 1 , Matilde Todaro 5 , Giulia Romano 6 , Renato Thomas 7 , Giuseppina Cortino 7 , Miriam Gaggianesi 5 , Manel Esteller 4 , Carlo M. Croce 6 , Gerolama Condorelli 1, 2 1 Department of Molecular Medicine and Medical Biotechnology, “Federico II” University of Naples, Naples, Italy 2 IEOS-CNR, Naples, Italy 3 IRCCS-SDN, Naples, Italy 4 Epigenetic and Cancer Biology Program (PEBC) IDIBELL, Hospital Duran I Reynals, Barcelona, Spain 5 Department of Surgical and Oncological Sciences, Cellular and Molecular Pathophysiology Lab…

MicroRNA-29b-1 impairs in vitro cell proliferation, self‑renewal and chemoresistance of human osteosarcoma 3AB-OS cancer stem cells

2014

Osteosarcoma (OS) is the most common type of bone cancer, with a peak incidence in the early childhood. Emerging evidence suggests that treatments targeting cancer stem cells (CSCs) within a tumor can halt cancer and improve patient survival. MicroRNAs (miRNAs) have been implicated in the maintenance of the CSC phenotype, thus, identification of CSC-related miRNAs would provide information for a better understanding of CSCs. Downregulation of miRNA-29 family members (miR-29a/b/c; miR‑29s) was observed in human OS, however, little is known about the functions of miR-29s in human OS CSCs. Previously, during the characterization of 3AB-OS cells, a CSC line selected from human OS MG63 cells, we…

Genetic and Molecular Characterization of The Human Osteosarcoma 3AB-OS Cancer Stem Cell Line: A Possible Model For Studying Osteosarcoma Origin and …

2013

Finding new treatments targeting cancer stem cells (CSCs) within a tumor seems to be critical to halt cancer and improve patient survival. Osteosarcoma is an aggressive tumor affecting adolescents, for which there is no second-line chemotherapy. Uncovering new molecular mechanisms underlying the development of osteosarcoma and origin of CSCs is crucial to identify new possible therapeutic strategies. Here, we aimed to characterize genetically and molecularly the human osteosarcoma 3AB-OS CSC line, previously selected from MG63 cells and which proved to have both in vitro and in vivo features of CSCs. Classic cytogenetic studies demonstrated that 3AB-OS cells have hypertriploid karyotype wit…

Expresión de microRNAs en la zonas de transición epitelio mesenquimal en el carcinoma colorectal como factor inmunomodulador y pronóstico. Estudio cl…

2018

El subgrupo mesenquimal (CMS4) de la clasificación molecular de consenso de cáncer colorectal es el tipo más agresivo y de peor pronóstico, y se caracteriza por la sobre expresión de genes implicados en el mecanismo de transición epitelio mesenquimal (TEM). La desdiferenciación tumoral focal (DTF) es el reflejo histológico del proceso de TEM. En base a estos conocimientos nos planteamos explorar la influencia de la DTF en la expresión de genes y microRNAs implicados en el proceso de TEM y evaluar su utilidad como factores predictivos de supervivencia. Analizamos de forma retrospectiva el grado de DTF en una serie de 125 especímenes de colectomía en todos los estadios clínicos. Evaluamos la …

Impact of periodontal treatment on GCF miRNAs expression changes associated with cardiovascular risk in patients with periodontitis

2022

Prognostic Impact of let-7e MicroRNA and Its Target Genes in Localized High-Risk Intestinal GIST: A Spanish Group for Research on Sarcoma (GEIS) Study

2020

MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level, and they have been described as being associated with tumor prognosis. Here, miRNA profiling was planned to explore new molecular prognostic biomarkers in localized intestinal high-risk GIST. Paraffin tumor blocks of 14 and 86 patients were used in the discovery and expansion sets, respectively. GeneChip miRNA v3.0 was employed to identify the miRNAs differentially expressed between relapsed and non-relapsed patient samples, which were validated in the expansion set, by qRT-PCR. RT2 Profiler PCR Array was used for the screening of let-7e targets. Expression levels were co…