Search results for "ROLE"

showing 10 items of 1994 documents

Pyrocoll, an Antibiotic, Antiparasitic and Antitumor Compound Produced by a Novel Alkaliphilic Streptomyces Strain

2003

A new secondary metabolite was detected in the culture extract of Streptomyces sp. AK 409 by HPLC-diode-array screening. The metabolite was identified as pyrocoll, which is known to be a constituent of cigarette smoke. Pyrocoll is known as a synthetic compound, but until now had not been isolated as a natural product from a microorganism. The compound showed biological activity against various Arthrobacter strains, filamentous fungi, several pathogenic protozoa, and some human tumor cell lines.

Spectrophotometry InfraredAntiparasiticmedicine.drug_classMetaboliteAntiprotozoal AgentsMicrobial Sensitivity TestsSecondary metaboliteStreptomycesMass SpectrometryMicrobiologyMicechemistry.chemical_compoundArthrobacterDrug DiscoverymedicineAnimalsHumansPyrrolesNuclear Magnetic Resonance BiomolecularChromatography High Pressure LiquidSoil MicrobiologyAntibacterial agentPharmacologyAntibiotics AntineoplasticbiologyStreptomycetaceaebiology.organism_classificationStreptomyceschemistryFermentationChromatography GelActinomycetalesDrug Screening Assays AntitumorHeLa Cellsmedicine.drugThe Journal of Antibiotics
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Coupling of Action-Perception Brain Networks during Musical Pulse Processing: Evidence from Region-of-Interest-Based Independent Component Analysis

2017

Our sense of rhythm relies on orchestrated activity of several cerebral and cerebellar structures. Although functional connectivity studies have advanced our understanding of rhythm perception, this phenomenon has not been sufficiently studied as a function of musical training and beyond the General Linear Model (GLM) approach. Here, we studied pulse clarity processing during naturalistic music listening using a data-driven approach (independent component analysis; ICA). Participants’ (18 musicians and 18 controls) functional magnetic resonance imaging (fMRI) responses were acquired while listening to music. A targeted region of interest (ROI) related to pulse clarity processing was defined…

Speech recognitionMusiciansRhythm perceptionBehavioral Neuroscience0302 clinical medicinemedia_commonOriginal ResearchmuusikotFunctional integration (neurobiology)medicine.diagnostic_test05 social sciencesmusicianscerebral structurePulse (music)Psychiatry and Mental healthNeuropsychology and Physiological PsychologyNeurologyforecaststa6131Psychologyaivotcerebellar structureärsykkeetmedia_common.quotation_subjectbrainAuditory areamusiikkinaturalisticta3112rhythmbehavioral disciplines and activities050105 experimental psychologylcsh:RC321-57103 medical and health sciencesRhythmRegion of interestPerceptionmedicine0501 psychology and cognitive sciencesmusicstimuli (role related to effect)lcsh:Neurosciences. Biological psychiatry. NeuropsychiatryBiological Psychiatryfunctional magnetic resonance imaging (fMRI)ennusteetIndependent Component Analysis (ICA)predictionIndependent component analysisrytmirhythm perceptionFunctional magnetic resonance imagingindependent component analysis (ICA)030217 neurology & neurosurgeryNeuroscienceFrontiers in Human Neuroscience
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Pyrrolomycins as antimicrobial agents. Microwave-assisted organic synthesis and insights into their antimicrobial mechanism of action

2019

Abstract New compounds able to counteract staphylococcal biofilm formation are needed. In this study we investigate the mechanism of action of pyrrolomycins, whose potential as antimicrobial agents has been demonstrated. We performed a new efficient and easy method to use microwave organic synthesis suitable for obtaining pyrrolomycins in good yields and in suitable amount for their in vitro in-depth investigation. We evaluate the inhibitory activity towards Sortase A (SrtA), a transpeptidase responsible for covalent anchoring in Gram-positive peptidoglycan of many surface proteins involved in adhesion and in biofilm formation. All compounds show a good inhibitory activity toward SrtA, havi…

Staphylococcus aureusClinical BiochemistryPharmaceutical ScienceMicrobial Sensitivity Testsmedicine.disease_causeSettore BIO/19 - Microbiologia Generale01 natural sciencesBiochemistrychemistry.chemical_compoundBacterial ProteinsDrug DiscoverymedicinePyrrolesEnzyme InhibitorsMicrowavesMolecular BiologyEnzyme Assays010405 organic chemistryChemistryOrganic ChemistryBiofilmN-Acetylmuramoyl-L-alanine AmidaseAntimicrobialAminoacyltransferasesAntimicrobial resistance Pyrrolomycins Sortase A Staphylococcus aureus In-silico docking studies MAOS Pharmacokinetics studies Murein hydrolase activitySettore CHIM/08 - Chimica Farmaceutica0104 chemical sciencesAnti-Bacterial AgentsMolecular Docking Simulation010404 medicinal & biomolecular chemistryCysteine EndopeptidasesBiochemistryMechanism of actionDocking (molecular)Staphylococcus aureusSettore CHIM/03 - Chimica Generale E InorganicaSortase ABiofilmsPseudomonas aeruginosaMolecular MedicineOrganic synthesisPeptidoglycanmedicine.symptom
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Effect of temperature on the release of carvacrol and cinnamaldehyde incorporated into polymeric systems to control growth and biofilms ofEscherichia…

2015

This study assessed the effect of temperature on the release of essential oil components incorporated by melt compounding into polymeric films. Specifically, polyethylene-co-vinylacetate (EVA) films containing carvacrol (CAR) and cinnamaldehyde (ALD), alone and in combination, were prepared and their surface and mechanical properties and antibacterial and anti-biofilm activity against Escherichia coli and Staphylococcus aureus were evaluated. The addition of ALD and CAR did not provoke variation in the surface morphology of EVA and allowed their delivery. At 37°C, films containing CAR, ALD or their combination (25+75%) were found to have the strongest bactericidal effect, whereas at lower t…

Staphylococcus aureusMorphology (linguistics)Aquatic Sciencemedicine.disease_causeApplied Microbiology and BiotechnologybiofilmCinnamaldehydelaw.inventionchemistry.chemical_compoundlawOils VolatileEscherichia colimedicineOrganic chemistryCarvacrolAcroleinEscherichia coliEssential oilWater Science and TechnologyAcroleinBiofilmtemperatureAnti-Bacterial Agentsantibacterial polymerchemistryStaphylococcus aureusBiofilmsMonoterpenesStaphylococcus aureuCymenesPolyvinylsPolyethylenestemperature; antibacterial polymers; biofilm; essential oil components; Escherichia coli; Staphylococcus aureusessential oil componentNuclear chemistryBiofouling
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Pyrrolomycins as potential anti-staphylococcal biofilms agents

2010

With the goal of discovering new anti-infective agents active against microbial biofilms, this investigation focused on some natural pyrrolomycins, a family of halogenated pyrrole antibiotics. In this study the anti-staphylococcal biofilm activity of pyrrolomycins C, D, F1, F2a, F2b, F3 and of the synthesized related compounds I, II, III were investigated. The susceptibility of six staphylococcal biofilms was determined by methyltiazotetrazolium staining. Most of the compounds were active at concentrations of 1.5 microg ml(-1) with significant inhibition percentages. A few of the compounds were active at the lowest screening concentration of 0.045 microg ml(-1). The population log reduction…

Staphylococcus aureusSynthetic derivativesmedicine.drug_classCell SurvivalAntibioticsPopulationMicrobial Sensitivity TestsAquatic ScienceBiologymedicine.disease_causeSettore BIO/19 - Microbiologia GeneraleApplied Microbiology and BiotechnologyPolymerase Chain ReactionBacterial AdhesionMicrobiologyCell LineInhibitory Concentration 50medicineStaphylococcus epidermidisHumansPyrroleseducationWater Science and TechnologyMicrobial BiofilmsCell Proliferationeducation.field_of_studyMolecular StructureBiofilmStainingAnti-Bacterial AgentsStaphylococcal biofilms Anti-biofilm agents PyrrolomycinsStaphylococcus aureusBiofilmsToxicity
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Lo spazio logico della metafisica

2018

Partendo dal rapporto con il "campo dei concetti" descritto da Kant nella "Critica del giudizio", si riflette sul campo della metafisica individuato da Nicolaci come uno spazio, proprio della filosofia nel suo ruolo eminentemente critico,e come un compito specifico della metafisica

Starting from the relationship with the "field of concepts" described by Kant in the "Critique of Judgment" the essay reflects on the metaphysical field identified by Nicolaci as a space proper to philosophy in its eminently critical role and as a specific task of metaphysics
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Assessing covariate imbalance in meta-analysis studies.

2010

The main goal of meta-analysis is to combine data across studies or data sets to obtain summary estimates. In this paper, the novelty is to propose a statistical tool to assess a possible covariate imbalance in baseline variables to investigate similarity of trials. We conducted the detection of the covariate imbalance, first, through some graphical comparison of the empirical cumulative distribution functions or ECDFs, which are built by putting together arms or trials according to some risk factor, and second, through some non-parametric tests such as the Kolmogorov–Smirnov and the Anderson–Darling tests. To overcome the huge presence of ties, we conducted the statistical tests on perturbe…

Statistics and ProbabilityMaleperturbationEpidemiologyComputer sciencePoolingHypercholesterolemiaAlpha interferonMeta-Analysis as TopicCovariateStatisticsEconometricsHumansSettore SECS-S/05 - Statistica SocialeECDFnon-parametric testStatistical hypothesis testingRandomized Controlled Trials as TopicCumulative distribution functionNonparametric statisticsNoveltyInterferon-alphacombinabilityHepatitis C ChronicMeta-analysisData Interpretation StatisticalFemaleHydroxymethylglutaryl-CoA Reductase InhibitorsStatistics in medicine
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Bioactive pyrrole alkaloids isolated from the Red Sea : marine sponge Stylissa carteri

2017

Abstract Fifteen pyrrole alkaloids were isolated from the Red Sea marine sponge Stylissa carteri and investigated for their biological activities. Four of them were dibrominated [(+) dibromophakelline, Z-3-bromohymenialdisine, (±) ageliferin and 3,4-dibromo-1H-pyrrole-2-carbamide], nine compounds were monobrominated [(−) clathramide C, agelongine, (+) manzacidin A, (−) 3-bromomanzacidin D, Z-spongiacidin D, Z-hymenialdisine, 2-debromostevensine, 2-bromoaldisine and 4-bromo-1H-pyrrole-2-carbamide)] and finally, two compounds were non-brominated derivatives viz., E-debromohymenialdisine and aldisine. The structure elucidations of isolated compounds were based on 1D & 2D NMR spectroscopic …

StereochemistryCell Survival01 natural sciencesGeneral Biochemistry Genetics and Molecular BiologyCell LineRSchemistry.chemical_compoundAlkaloidsAnimalsHumansPyrrolesProtein kinase ACytotoxicityIndian OceanAgeliferinPyrrolebiologyMolecular Structure010405 organic chemistryKinasebiology.organism_classificationHCT116 Cells0104 chemical sciencesPorifera010404 medicinal & biomolecular chemistrySpongechemistryStylissa carteriDrug Screening Assays AntitumorTwo-dimensional nuclear magnetic resonance spectroscopy
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Annelated pyrrolo-pyrimidines from amino-cyanopyrroles and BMMAs as leads for new DNA-interactive ring systems.

2005

The efficient one-pot synthesis of several new tricyclic systems of type 1 and 2, obtained from the reaction of substituted 2-amino-3-cyanopyrroles and 3-amino-4-cyanopyrroles with BMMAs, is reported. The duration and yields of the reaction strongly depend on the reactivity of the starting pyrrole and on the size of the ring to be formed. Mechanist features of the reaction were investigated and proposed by studying also the reactivity of a 3-aminopyrrole-2,4-dicyano substituted. The method reported represents the first example of the use of BMMA reagents in combination with pyrrole derivatives and allows an easy and versatile entry to a large number of hitherto unknown pyrrolo-pyrimidines f…

StereochemistryClinical BiochemistryPharmaceutical ScienceRing (chemistry)Annelated pyrrolo-pyrimidineBiochemistryChemical synthesisPyrrole derivativeschemistry.chemical_compoundBMMA reagentDrug DiscoveryReactivity (chemistry)PyrrolesMolecular BiologyPyrroleSpectrum AnalysisOrganic ChemistryDNAAmino-cyanopyrroleCombinatorial chemistryPyrimidineschemistryDNA-interactive polycycles.ReagentOne pot reactionMolecular MedicineDNABioorganicmedicinal chemistry
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Pyrrolo[2,3-h]quinolinones: synthesis and photochemotherapic activity.

2003

A series of derivatives of the new ring system pyrrolo[2,3-h]quinoline-2-one was synthesized and evaluated as photoreagents toward cultured human tumor cells. Remarkable phototoxycity resulted for some derivatives, especially those bearing the phenyl group at the 7-position.

StereochemistryFibrosarcomaClinical BiochemistryPharmaceutical ScienceAntineoplastic AgentsQuinolonesRing (chemistry)BiochemistryChemical synthesischemistry.chemical_compoundStructure-Activity RelationshipDrug DiscoveryTumor Cells CulturedPhenyl groupHumansPhotosensitizerPyrrolesMolecular BiologyPhotosensitizing AgentsChemistryOrganic ChemistryGeneral MedicineIn vitroHuman tumorPhotochemotherapyCell cultureLactamMolecular MedicineDrug Screening Assays AntitumorBioorganicmedicinal chemistry letters
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