Search results for "ROMA"
showing 10 items of 13919 documents
Baculovirus-mediated immediate-early gene expression and nuclear reorganization in human cells
2007
Baculovirus, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), has the ability to transduce mammalian cell lines without replication. The general objective of this study was to detect the transcription and expression of viral immediate-early genes in human cells and to examine the interactions between viral components and subnuclear structures. Viral capsids were seen in large, discrete foci in nuclei of both dividing and non-dividing human cells. Concurrently, the transcription of viral immediate-early transregulator genes (ie-1, ie-2) and translation of IE-2 protein were detected. Quantitative microscopy imaging and analysis showed that virus transduction altered the size of …
Quantitative microscopy reveals stepwise alteration of chromatin structure during herpesvirus infection
2019
During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening arou…
Caracterización del viroma en plasma de donantes de sangre
2022
El desarrollo de la metagenómica ha impulsado el descubrimiento de un número notable de nuevas secuencias virales, lo que ha permitido conocer la composición de las comunidades virales de múltiples ambientes, entre los que se incluye el cuerpo humano. El viroma humano se caracteriza por presentar una enorme heterogeneidad, en cada localización corporal existe un ambiente determinado, lo que se refleja en una composición muy diversa de las comunidades de virus y bacterias presentes. En concreto, el plasma sanguíneo se caracteriza por albergar diferentes virus, incluso en individuos aparentemente sanos, como los donantes de sangre. Para caracterizar los virus presentes en muestras de plasma, …
Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging
2018
International audience; Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200pM of beta-ionone) and in molecular weight of …
Micro-determination of dithiocarbamates in pesticide formulations using voltammetry
2013
Abstract The purpose of this work was to develop a reliable method for the micro-determination of dithiocarbamates (mancozeb, maneb, propineb, nabam, Na(CH3)2DTC, zineb, ziram, ferbam and thiram) in pesticide formulations for agriculture using adsorptive stripping voltammetry (AdSV). The accuracy of analytical method was valued by analyzing simulate formulation samples prepared by us with known amounts of active ingredients. In addition, the applicability of the AdSV procedure for the analysis of DTCs and EBDTCs in micro-samples was evaluated by estimating its recoveries from spiked commercial formulate samples. The accuracy, valuated as recovery percentage ranged between 85% and 97%. The p…
Voltammetric determination of platinum in perfusate and blood: Preliminary data on pharmacokinetic study of arterial infusion with oxaliplatin
2012
Abstract Because Platinum Group Elements have found widespread use in catalytic converters in cars and as chemotherapeutic agent, interest in the development of reliable analytical methods is carried out in order to monitor these analita in humans to protect the citizen's health. Considering that information on the levels of many trace elements in biological matrices is scarce and for many non-essential elements, baseline levels in the population, and especially in those particularly exposed to the risk are lacking, in this paper we optimize an analytical method for biological matrices, using a voltammetric technique to measure the concentration of Pt in blood and perfusate. The amount of P…
Language ideologies in the Romanian Banat : analysis of interviews and academic writings among the Hungarians and Germans
2009
This is an introduction to a compilation of six articles on language ideologies in the Romanian Banat. My dissertation was initiated by fieldwork in the area, resulting to a database of interviews among the Hungarian and German inhabitants. This data is compared to intellectual writings. With regards to researcher position, I identify myself as a traveler in the area and as a foreign scholar. The analytical framework of this study draws from the study of Language Ideologies and Conversation Analysis.Here, language ideologies are broadly defined as metalinguistic discourse or talk about language. In the analysis of interviews, conversation analysis is used, in order to analyze the emergent c…
L'Hostalot (La Vilanova d'Alcolea, Castellón). Una posible estación de la Vía Augusta
1989
Avance de los resultados obtenidos en las campañas de excavación realizadas durante los años 1986-87 en el yacimiento romano de L'Hostalot (Vilanova d'Alcolea, Castellón), que se identifica con la posta Ildum de la Vía Augusta.
MELNBALTĀ FOTO IZMANTOŠANAS KALENDĀRU DIZAINĀ VIZUĀLI STILISTISKIE ASPEKTI
2019
Diplomdarba “Melnbaltā foto izmantošanas kalendāru dizainā vizuāli stilistiskie aspekti” teorētiskajā daļā autore pēta fotogrāfijas vēsturi, melnbaltās fotogrāfijas izveides principus, kalendāru vēsturi un to veidus. Darba pētnieciskajā daļā pēc vairākiem kritērijiem tiek analizēti 21. gadsimta melnbalto fotokalendāru dizainparaugi no Latvijas un visas pasaules. Darba radošajā daļā tiek izveidots praktiski pielietojams melnbalts foto kalendārs ar kaķu portretiem. Darbs sastāv no 77 lappusēm, 68 attēliem un 1. tabulas. Tā izveidē izmantoti 62 avoti.
Gli affreschi provenienti dalle terme romane di Reggio Calabria. Dalla conoscenza al restauro
2023
The frescoes at marine thermae discovered in 1886 during the excavation of the small thermal complex dating between the 1th and 3rd century AD, and located on the seafront of Reggio Calabria, have been collected already collapsed. They are currently part of the permanent exhibition of the National Archaeological Museum of Reggio Calabria. This paper illustrates the results of the restoration intervention, carried out in the form of an educational activity in the context of an agreement signed between the MarRC and the University of Calabria, and the results of the diagnostic investigations carried out by the IPCF-CNR of Messina and the STEBICEF department of the University of Palermo.