Search results for "Reactive"
showing 10 items of 1469 documents
Controlled Cytotoxicity of Plasma Treated Water Formulated By Open-air Hybrid Mode Discharge
2017
Plasma‐activated liquids (PAL) attract increasing interest with demonstrated biological effects. Plasma exposure in air produces stable aqueous reactive species which can serve as chemical diagnostics of PAL systems. Here, we tailor aqueous reactive species inside plasma‐activated water (PAW) through treating water with AC air spark and glow discharges in contact with water. Chemical probing demonstrated species specificity between two types of PAW. Spark discharge PAW contains urn:x-wiley:14381656:media:ppap201600207:ppap201600207-math-0006 and urn:x-wiley:14381656:media:ppap201600207:ppap201600207-math-0007, while urn:x-wiley:14381656:media:ppap201600207:ppap201600207-math-0008and urn:x-w…
Turbulent jet through porous obstructions under Coriolis effect: an experimental investigation
2021
AbstractThe present study has the main purpose to experimentally investigate a turbulent momentum jet issued in a basin affected by rotation and in presence of porous obstructions. The experiments were carried out at the Coriolis Platform at LEGI Grenoble (FR). A large and unique set of velocity data was obtained by means of a Particle Image Velocimetry measurement technique while varying the rotation rate of the tank and the density of the canopy. The main differences in jet behavior in various flow configurations were assessed in terms of mean flow, turbulent kinetic energy and jet spreading. The jet trajectory was also detected. The results prove that obstructions with increasing density…
Reactive oxygen species formed in aqueous mixtures of secondary organic aerosols and mineral dust influencing cloud chemistry and public health in th…
2017
Mineral dust and secondary organic aerosols (SOA) account for a major fraction of atmospheric particulate matter, affecting climate, air quality and public health. How mineral dust interacts with SOA to influence cloud chemistry and public health, however, is not well understood. Here, we investigated the formation of reactive oxygen species (ROS), which are key species of atmospheric and physiological chemistry, in aqueous mixtures of SOA and mineral dust by applying electron paramagnetic resonance (EPR) spectrometry in combination with a spin-trapping technique, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and a kinetic model. We found that substantial amounts of ROS includi…
Transcriptome approach to understand the potential mechanisms inhibiting or triggering blossom-end rot development in tomato fruit in response to pla…
2017
The objectives of this study were to analyze changes in gene expression and identify candidate genes and gene networks potentially inhibiting or triggering blossom-end rot (BER) in tomatoes treated with plant growth regulators. ?Ace 55 (Vf)? tomato plants were grown in a greenhouse and sprayed with Apogee (300 mg L?1), abscisic acid (ABA) (500 mg L?1), water (control), or gibberellins 4?+?7 (GA4?+?7) (300 mg L?1) weekly after pollination. The BER incidence rate was zero in Apogee- and ABA-, medium in water-, and high in GA4?+?7-treated plants from 26 to 40 days after pollination (DAP). At 26 DAP, healthy blossom-end fruit tissue still not showing visible BER symptoms was used for transcript…
How water-soluble chlorophyll protein extracts chlorophyll from membranes.
2020
Water-soluble chlorophyll proteins (WSCPs) found in Brassicaceae are non-photosynthetic proteins that bind only a small number of chlorophylls. Their biological function remains unclear, but recent data indicate that WSCPs are involved in stress response and pathogen defense as producers of reactive oxygen species and/or Chl-regulated protease inhibitors. For those functions, WSCP apoprotein supposedly binds Chl to become physiologically active or inactive, respectively. Thus, Chl-binding seems to be a pivotal step for the biological function of WSCP. WSCP can extract Chl from the thylakoid membrane but little is known about the mechanism of how Chl is sequestered from the membrane into the…
Dehydration rate determines the degree of membrane damage and desiccation tolerance in bryophytes.
2016
Desiccation tolerant (DT) organisms are able to withstand an extended loss of body water and rapidly resume metabolism upon rehydration. This ability, however, is strongly dependent on a slow dehydration rate. Fast dehydration affects membrane integrity leading to intracellular solute leakage upon rehydration and thereby impairs metabolism recovery. We test the hypothesis that the increased cell membrane damage and membrane permeability observed under fast dehydration, compared with slow dehydration, is related to an increase in lipid peroxidation. Our results reject this hypothesis because following rehydration lipid peroxidation remains unaltered, a fact that could be due to the high incr…
An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidificat…
2020
Intracellular acid stress inhibits plant growth by unknown mechanisms and it occurs in acidic soils and as consequence of other stresses. In order to identify mechanisms of acid toxicity, we screened activation-tagging lines of Arabidopsis thaliana for tolerance to intracellular acidification induced by organic acids. A dominant mutant, sbt4.13-1D, was isolated twice and shown to over-express subtilase SBT4.13, a protease secreted into endoplasmic reticulum. Activity measurements and immuno-detection indicate that the mutant contains less plasma membrane H+-ATPase (PMA) than wild type, explaining the small size, electrical depolarization and decreased cytosolic pH of the mutant but not orga…
Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.
2017
Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…
Polyamine Biosynthesis Engineering as a Tool to Improve Plant Resistance to Abiotic Stress
2015
Polyamines (PAs) are small polycationic molecules which are present in all living organisms. PAs have been involved in a wide array of metabolic plant processes, extending from development to stress protection. Most of this knowledge has been achieved through the observation of PA homeostasis and manipulation of plant PA levels mediated by different approaches. This chapter summarizes the approaches undertaken to demonstrate the relationship between PAs and the stress response and, in particular, how the genetic manipulation of polyamine levels has evolved in a useful tool for the enhancement of plant stress tolerance in many species, including crops. This chapter also includes the most rec…
The Effect of Cadmium on Oxidative Stress in Beta vulgaris
2018
Abstract As a heavy metal, cadmium has strongly toxic effects on plants and can induce oxidative stress. It is absorbed by the roots and transported to the stems and leaves. The aim of the study was to evaluate the effect of various concentrations of cadmium on the metabolic activity of Beta vulgaris and assess the dependence of these processes on the content of metal in the plants. To demonstrate the effect of cadmium on metabolism, protein and photosynthetic pigment content, lipid peroxidation, and the activity of enzymes specific for oxidative stress in roots and shoots were measured. Seeds of B. vulgaris were treated with different concentrations of Cd supplied via a CdCl2 solution: 0 (…