Search results for "Real-Time"
showing 10 items of 881 documents
Evidence for shifts in the structure and abundance of the microbial community in a long-term PCB-contaminated soil under bioremediation.
2011
International audience; Although the impact of bioremediation of PCB-contaminated sites on the indigenous microbial community is a key question for soil restoration, it remains poorly understood. Therefore, a small-scale bioremediation assay made of (a) a biostimulation treatment with carvone, soya lecithin and xylose and (b) two bioaugmentation treatments, one with a TSZ7 mixed culture and another with a Rhodococcus sp. Z6 pure strain was set up. Changes in the structure of the global soil microbial community and in the abundances of different taxonomic phyla were monitored using ribosomal intergenic spacer analysis (RISA) and real-time PCR. After an 18-month treatment, the structure of th…
Following ionic activity by electrochemistry during the polymerase chain reaction
2009
The most commonly used technique for gene detection is the polymerase chain reaction (PCR). PCR is associated with alterations in ionic activity because inorganic pyrophosphate (PPi) and inorganic phosphate (Pi) ions are produced during nucleotide polymerization. To maintain electro-neutrality, magnesium, potassium, and ammonium ions are bound to DNA. Deoxynucleotides are also bound to DNA during PCR. Some authors have described DNA itself as an electrically conducting polymer formed by base stapling with the formation of extensive Pi systems. In the current study, alterations in electrical conductivity determined experimentally during PCR are reported, and a model explaining the observed c…
Invariant NKT cells are expanded in peripheral blood but are undetectable in salivary glands of patients with primary Sjögren's syndrome
2016
OBJECTIVES: Invariant NKT (iNKT) cells play a role in regulating the function of autoreactive B cells before their entry into germinal centres. Absence and/or reduction of iNKT cells have been demonstrated in patients with systemic lupus erythematosus (SLE) together with an increase of autoreactive B cell activity. Primary Sjögren's syndrome (pSS) is a systemic autoimmune disease in which lymphocyte infiltration and organisation in lymphoid structures of inflamed salivary glands occurs. The aim of the study was to investigate the percentage and function of iNKT in the salivary glands and peripheral blood of patients with pSS. METHODS: Minor salivary gland biopsies were obtained from patient…
Postnatal overfeeding in rats leads to moderate overweight and to cardiometabolic and oxidative alterations in adulthood.
2011
In contrast to the masses of data on obesity, few data are available concerning the cardiometabolic and oxidative consequences of moderate overweight. The model of postnatal overfeeding (OF) induces an increase in body weight at weaning that remains during adult life. Litters of Wistar rats were either maintained at 12 pups (normal-fed group, NF), or reduced to 3 pups at birth in order to induce OF. At 6 months of age, metabolic parameters, circulating oxidative stress and aortic and coronary vasoreactivity were assessed. Cardiac susceptibility to ischemia-reperfusion injury was also evaluated ex vivo as were markers of cardiac remodeling. OF led to an increase in body weight at weaning (+5…
ERK1 and ERK2 activation modulates diet-induced obesity in mice
2017
IF 3.112; International audience; Obesity is a worldwide problem, and dietary lipids play an important role in its pathogenesis. Recently, Erk1 knock-out (ERK1(-/-)) mice have been shown to exhibit low preference for dietary fatty acids. Hence, we maintained Erk1(-/-) mice on a high-fat diet (HFD) to assess the implication of this mitogen-activated protein (MAP) kinase in obesity. The Erk1(-/-) mice, fed the HFD, were more obese than wild-type (WT) animals, fed the same diet. Erk1(-/-) obese mice gained more fat and liver mass than WT obese animals. No difference was observed in daily food and energy intake in HFD-fed both group of animals. However, feed efficiency was higher in Erk1(-/-) t…
The down-regulation of miR-125b in chronic lymphocytic leukemias leads to metabolic adaptation of cells to a transformed state
2012
AbstractMiR-125b-1 maps at 11q24, a chromosomal region close to the epicenter of 11q23 deletions in chronic lymphocytic leukemias (CLLs). Our results establish that both aggressive and indolent CLL patients show reduced expression of miR-125b. Overexpression of miR-125b in CLL-derived cell lines resulted in the repression of many transcripts encoding enzymes implicated in cell metabolism. Metabolomics analyses showed that miR-125b overexpression modulated glucose, glutathione, lipid, and glycerolipid metabolism. Changes on the same metabolic pathways also were observed in CLLs. We furthermore analyzed the expression of some of miR-125b–target transcripts that are potentially involved in the…
Three Step Bluetooth Positioning
2005
This paper discusses a three step procedure to perform high definition positioning by the use of low cost Bluetooth devices. The three steps are: Sampling, Deployment, and Real Time Positioning. A genetic algorithm is discussed for deployment optimization and a neural network for real time positioning. A case study, along with experiments and results, are finally discussed dealing with a castle in Sicily where many trials were carried out to the end of arranging a positioning system for context aware service provision to visitors.
Fast prototyping of a SoC-based smart-camera: a real-time fall detection case study
2014
International audience; Smart camera, i.e. cameras that are able to acquire and process images in real-time, is a typical example of the new embedded computer vision systems. A key example of application is automatic fall detection, which can be useful for helping elderly people in daily life. In this paper, we propose a methodology for development and fast-prototyping of a fall detection system based on such a smart camera, which allows to reduce the development time compared to standard approaches. Founded on a supervised classification approach, we propose a HW/SW implementation to detect falls in a home environment using a single camera and an optimized descriptor adapted to real-time t…
Embedded System Study for Real Time Boosting Based Face Detection
2006
This paper describes a study for a real time embedded face detection system. Recently, the boosting based face detection algorithms proposed by [(Viola, P and Jone, M, 2001); (Lienhart, R, et al., 2003)] have gained a lot of attention and are considered as the fastest accurate face detection algorithms today. However, the embedded implementation of such algorithms into hardware is still a challenge, since these algorithms are heavily based on memory access. A sequential implementation model is built showing its lack of regularity in time consuming and speed of detection. We propose a parallel implementation that exploits the parallelism and the pipelining in these algorithms. This implement…
Real Time Robust Embedded Face Detection Using High Level Description
2011
Face detection is a fundamental prerequisite step in the process of face recognition. It consists of automatically finding all the faces in an image despite the considerable variations of lighting, background, appearance of people, position/orientation of faces, and their sizes. This type of object detection has the distinction of having a very large intra-class, making it a particularly difficult problem to solve, especially when one wishes to achieve real time processing. A human being has a great ability to analyze images. He can extract the information about it and focus only on areas of interest (the phenomenon of attention). Thereafter he can detect faces in an extremely reliable way.…