Search results for "Real-Time"

A central role for Notch in effector CD8(+) T cell differentiation.

2014

Activated CD8(+) T cells choose between terminal effector cell (TEC) or memory precursor cell (MPC) fates. We found that the signaling receptor Notch controls this 'choice'. Notch promoted the differentiation of immediately protective TECs and was correspondingly required for the clearance of acute infection with influenza virus. Notch activated a major portion of the TEC-specific gene-expression program and suppressed the MPC-specific program. Expression of Notch was induced on naive CD8(+) T cells by inflammatory mediators and interleukin 2 (IL-2) via pathways dependent on the metabolic checkpoint kinase mTOR and the transcription factor T-bet. These pathways were subsequently amplified d…

RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues

2006

In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could co…

Corrigendum to “Spatial monitoring of gene activity in extraradical and intraradical developmental stages of arbuscular mycorrhizal fungi by direct f…

2008

Clinical virology of cytomegalovirus infection following hematopoietic transplantation

2010

Cytomegalovirus (CMV) causes significant morbidity and mortality in allogeneic stem cell transplant recipients. The incidence of early CMV disease has been dramatically reduced by use of preemptive therapeutic strategies. Nevertheless, a trend toward a later appearance of CMV disease is being increasingly recognized. Currently, quantitative real-time PCR assays are the first-line choice for monitoring active CMV infection. However, no threshold DNAemia levels for triggering the initiation of preemptive therapy have been clinically validated in large studies. Although more time limited than universal prophylaxis, preemptive therapy strategies may also result in over treatment. Combined viro…

Total RNA-isolation of abdominal hernia of rats for quantitative real-time reverse transcription (RT) PCR assays.

2007

Abstract Increasing complications in incisional hernia surgery call for novel treatments. A gene expression analysis of injured tissues displays important parameters for tissue regeneration. Until today, no reliable method has been described for a quantitative gene expression analysis of hernia tissues. In this work, a protocol is described for the isolation of DNA‐free total RNA of incisional hernias for the first time. Moreover, real‐time RT PCR assays for collagen type I and III and TGF‐β1 are demonstrated for relative gene expression analyses. Both methods enable relative gene expression analyses of hernia tissues for the first time.

Induction of cholesterol biosynthesis by archazolid B in T24 bladder cancer cells.

2014

Abstract Background Resistance of cancer cells towards chemotherapeutics represents a major cause of therapy failure. The objective of our study was to evaluate cellular defense strategies in response to the novel vacuolar H+-ATPase inhibitor, archazolid B. Experimental approach: The effects of archazolid B on T24 bladder carcinoma cells were investigated by combining “omics” technologies (transcriptomics (mRNA and miRNA) and proteomics). Free cholesterol distribution was determined by filipin staining using flow cytometry and fluorescence microscopy. Flow cytometry was performed for LDLR surface expression studies. Uptake of LDL cholesterol was visualized by confocal microscopy. SREBP acti…

FINDR: Low-Cost Indoor Positioning Using FM Radio

2009

This paper presents an indoor positioning system based on FM radio. The system is built upon commercially available, short-range FM transmitters. The features of the FM radio which make it distinct from other localisation technologies are discussed. Despite the low cost and off-the-shelf components, the performance of the FM positioning is comparable to that of other positioning technologies (such as Wi-Fi). From our experiments, the median accuracy of the system is around 1.3 m and in 95% of cases the error is below 4.5 m.

Improving RF Fingerprinting Methods by Means of D2D Communication Protocol

2019

Radio Frequency (RF) fingerprinting is widely applied for indoor positioning due to the existing Wi-Fi infrastructure present in most indoor spaces (home, work, leisure, among others) and the widespread usage of smartphones everywhere. It corresponds to a simple idea, the signal signature in a location tends to be stable over the time. Therefore, with the signals received from multiple APs, a unique fingerprint can be created. However, the Wi-Fi signal is affected by many factors which degrade the positioning error range to around a few meters. This paper introduces a collaborative method based on device-to-device (D2D) communication to improve the positioning accuracy using only fingerprin…

Computing Real-Time Dynamic Origin/Destination Matrices from Vehicle-to-Infrastructure Messages Using a Multi-Agent System

2012

Dynamic Origin/Destination matrices are one of the most important parameters for efficient and effective transportation system management. These matrices describe the vehicle flow between different points within a region of interest for a given period of time. Usually, dynamic O/D matrices are estimated from traffic counts provided by induction loop detectors, home interview and/or license plate surveys. Unfortunately, estimation methods take O/D flows as time invariant for a certain number of intervals of time, which cannot be suitable for some traffic applications. However, the advent of information and communication technologies (e.g., vehicle-to-infrastructure dedicated short range comm…

Viability RT-PCR for SARS-CoV-2: a step forward to solve the infectivity quandary

2021

Background Isolation, contact tracing and restrictions on social movement are being globally implemented to prevent and control onward spread of SARS-CoV-2, even though the infection risk modelled on RNA detection by RT-qPCR remains biased as viral shedding and infectivity are not discerned. Thus, we aimed to develop a rapid viability RT-qPCR procedure to infer SARS-CoV-2 infectivity in clinical specimens and environmental samples. Methods We screened monoazide dyes and platinum compounds as viability molecular markers on five SARS-CoV-2 RNA targets. A platinum chloride-based viability RT-qPCR was then optimized using genomic RNA, and inactivated SARS-CoV-2 particles inoculated in buffer, s…