Search results for "Real"

Effect of simulated faunal impoverishment and mixture on the ecological structure of modern mammal faunas: Implications for the reconstruction of Mio…

2011

15 pages; International audience; The strong link between environment and the ecological diversity of communities is often used for drawing palaeoenvironmental inferences from fossil assemblages. Here we focus on the reliability of fossil samples in comparison to original communities when inferring palaeoenvironments from the ecological diversity of fossil mammal faunas. Taphonomic processes and sampling techniques generally introduce two kinds of biases in fossil samples: 1) the directional impoverishment of communities, i.e. the absence of some specific categories of bones, individuals or species; and 2) the mixture of several communities, temporally (timeaveraging) and/or spatially (spac…

A Lack of Attribution: Closing the Citation Gap Through a Reform of Citation and Indexing Practices

2012

Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology

2020

In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview r…

Ultraviolet radiation accelerates photodegradation under controlled conditions but slows the decomposition of senescent leaves from forest stands in …

2019

Depending on the environment, sunlight can positively or negatively affect litter decomposition, through the ensemble of direct and indirect processes constituting photodegradation. Which of these processes predominate depends on the ecosystem studied and on the spectral composition of sunlight received. To examine the relevance of photodegradation for litter decomposition in forest understoreys, we filtered ultraviolet radiation (UV) and blue light from leaves of Fagus sylvatica and Bettda pendula at two different stages of senescence in both a controlled-environment experiment and outdoors in four different forest stands (Picea abies, Pagus sylvatica, Acer platanoides, Betula pendula). Co…

Ecological plant epigenetics: Evidence from model and non-model species, and the way forward

2017

Growing evidence shows that epigenetic mechanisms contribute to complex traits, with implications across many fields of biology. In plant ecology, recent studies have attempted to merge ecological experiments with epigenetic analyses to elucidate the contribution of epigenetics to plant phenotypes, stress responses, adaptation to habitat, and range distributions. While there has been some progress in revealing the role of epigenetics in ecological processes, studies with non-model species have so far been limited to describing broad patterns based on anonymous markers of DNA methylation. In contrast, studies with model species have benefited from powerful genomic resources, which contribute…

Identification of Stress Associated microRNAs in Solanum lycopersicum by High-Throughput Sequencing

2019

Tomato (Solanum lycopersicum) is one of the most important crops around the world and also a model plant to study response to stress. High-throughput sequencing was used to analyse the microRNA (miRNA) profile of tomato plants undergoing five biotic and abiotic stress conditions (drought, heat, P. syringae infection, B. cinerea infection, and herbivore insect attack with Leptinotarsa decemlineata larvae) and one chemical treatment with a plant defence inducer, hexanoic acid. We identified 104 conserved miRNAs belonging to 37 families and we predicted 61 novel tomato miRNAs. Among those 165 miRNAs, 41 were stress-responsive. Reverse transcription quantitative PCR (RT-qPCR) was used to valida…

Standardised spider (Arachnida, Araneae) inventory of Lammi, Finland

2020

In June 2019, an ecology field course of the University of Helsinki was held at Lammi Biological Station, Southern Finland. Within this course, the students familiarised themselves with field work and identification of spiders and explored the diversity of species in the area. Three sampling plots were chosen, one in grassland and two in boreal forest, to demonstrate the sampling techniques and, by applying a standardised protocol (COBRA), contribute to a global spider biodiversity project. The collected samples contained a total of 3445 spiders, of which 1956 (57%) were adult. Only adult spiders were accounted for in the inventory due to the impossibility of identification of juveniles. A …

Isolation of acetic, propionic and butyric acid-forming bacteria from biogas plants.

2015

In this study, acetic, propionic and butyric acid-forming bacteria were isolated from thermophilic and mesophilic biogas plants (BGP) located in Germany. The fermenters were fed with maize silage and cattle or swine manure. Furthermore, pressurized laboratory fermenters digesting maize silage were sampled. Enrichment cultures for the isolation of acid-forming bacteria were grown in minimal medium supplemented with one of the following carbon sources: Na(+)-dl-lactate, succinate, ethanol, glycerol, glucose or a mixture of amino acids. These substrates could be converted by the isolates to acetic, propionic or butyric acid. In total, 49 isolates were obtained, which belonged to the phyla Firm…

Wood-inhabiting fungi with tight associations with other species have declined as a response to forest management

2017

Research on mutualistic and antagonistic networks, such as plant-pollinator and host-parasite networks, has shown that species interactions can influence and be influenced by the responses of species to environmental perturbations. Here we examine whether results obtained for directly observable networks generalize to more complex networks in which species interactions cannot be observed directly. As a case study, we consider data on the occurrences of 98 wood-inhabiting fungal species in managed and natural forests. We specifically ask if and how much the positions of wood-inhabiting fungal species within the interaction networks influence their responses to forest management. For this, we…

Sedimentary Ancient DNA (sedaDNA) Reveals Fungal Diversity and Environmental Drivers of Community Changes throughout the Holocene in the Present Bore…

2021

Fungi are ecologically important in several ecosystem processes, yet their community composition, ecophysiological roles, and responses to changing environmental factors in historical sediments are rarely studied. Here we explored ancient fungal DNA from lake Lielais Svētiņu sediment throughout the Holocene (10.5 kyr) using the ITS metabarcoding approach. Our data revealed diverse fungal taxa and smooth community changes during most of the Holocene with rapid changes occurring in the last few millennia. More precisely, plankton parasitic fungi became more diverse from the Late Holocene (2–4 kyr) which could be related to a shift towards a cooler climate. The Latest Holocene (~2 kyr) showed …