Search results for "Receptor"

Diversity of TonB-dependent outer-membrane proteins in plant-associated strains of Pseudomonas fluorescens

2011

International audience; Genomic sequences of ten strains of plant-associated Pseudomonas spp. were surveyed for the presence of TonB-dependent outer-membrane proteins (TBDPs), which function in the uptake of substrates from the environment by many Gram-negative bacteria. The ten strains represent P. fluorescens, P. chlororaphis, and P. synxantha isolated from the phyllosphere, rhizosphere or soil. 14 to 45 TBDPs were identified in each strain, and phylogenetic analysis of the TBDPs identified five that are conserved across all ten genomes. Comparisons to proteins with known functions allowed the assignment of putative roles in uptake of heme, vitamin B12, copper, and the siderophore ferrich…

Thiourea Based Tritopic Halogen Bonding Acceptors

2023

Series of thiourea based tritopic receptor molecules were synthesized to be used as building blocks for halogen-bonded assemblies. Here 16 new receptor molecules were synthesized from two different 2,4,6-trialkyl-1,3,5-tris(bromomethyl)benzene starting materials via tris(isothiocyanatomethyl)benzene intermediates. The alkyl substituents in the benzene ring showed to be important for isothiocyanate group formation instead of competing thiocyanate group. The synthesis route allowed us to synthesize the isothiocyanate intermediates and further the receptor molecules without typically used and highly toxic thiophosgene. Synthesized receptor molecules were used to study their halogen bond accept…

Dažādu vielu efekti uz kalcija līmeni H9C2 šūnās

2017

Kalcija joniem ir liela bioloģiskā loma vairāku šūnu procesu regulācijā un aktivācijā. Kalcija līmeni mēra meklējot jaunus kalcija antagonistus, pētot vielu darbības mehānismus, kā arī pētot ilgstošas intracelulārā kalcija līmeņa paaugstināšanās izraisītu citotoksicitāti. Paaugstināts kalcija līmenis izraisa gludās muskulatūras pastiprinātas kontrakcijas, kas noved pie asinsvadu sašaurināšanās un paaugstināta asinsspiediena. Lai normalizētu kardiovaskulāro darbību lieto kalcija antagonistus, piemēram, nifedipīnu un verapamilu. Pētījuma mērķis bija optimizēt kalcija līmeņa mērīšanas metodi, izmantojot zināmus kalcija antagonistus kā references vielas un pētīt gamma aminosviestskābes receptor…

From isolated 1H-pyrazole cryptand anion receptors to hybrid inorganic-organic 1D helical polymeric anion receptors

2015

We report a novel 1-D helical coordination polymer formed by protonated polyamine 1H-pyrazole cryptands interconnected by Cu2+ metal ions that are able to encapsulate anionic species behaving as a multianion receptor. Switching from a monomeric receptor to a polymeric receptor is activated by metal ions and pH.

Adenosine A2A and A2B Receptors Differentially Modulate Keratinocyte Proliferation: Possible Deregulation in Psoriatic Epidermis

2017

Adenosine is a potent regulator of inflammation and immunity, but the role of adenosine receptors in keratinocytes remains controversial. We determined that in addition to A2B receptors, human epidermal keratinocytes also express A2A receptors, although to a lower extent. Through the use of selective adenosine receptor agonists and antagonists, we showed that physiological concentrations of adenosine activate A2B receptors in normal human keratinocytes, inducing cell cycle arrest through the increase of intracellular calcium but not through cAMP signaling. In contrast, the selective activation of A2A receptors by CGS-21680 induces keratinocyte proliferation via p38–mitogen-activated protein…

Fluorescent quantum dot labeling of canine transferrin receptor with biotin ligase

2007

N-galā ieviestas hemaglutinīna iezīmes ietekme uz melanokortīnu receptoru daudzumu šūnas membrānā

2018

Darbā tika veikta ar hemaglutinīna (HA) iezīmi N-galā un zaļo fluorescento proteīnu (GFP) C- galā sajūgtu melanokortīna 2. tipa (MC2R) un 4. tipa (MC4R) sekvenču kodējošu ekspresijas plazmīdu izveide. Jauniegūtajiem receptoriem tika noteikta funkcionālā aktivitāte CHO (Chinese Hamster Ovary cells) šūnās, lai pārliecinātos par to atbilstību izmantošanai receptoru šūnu virsmas ekspresijas mērījumos. Darba mērķis bija izmantot šos receptorus, lai pārbaudītu HA iezīmes un GFP ietekmi uz receptoru CHO virsmas ekspresiju, izmantojot konfokālo lāzerskenējošo mikroskopiju un imunofluorescento testu attiecīgi. Darbā tehnisku sarežģījumu dēļ netika pārbaudīta GFP ietekme, bet novērotā HA ietekme mazi…

Exercise and lactate production:implication in fatigue and in brain signaling

2016

More than two hundred years after its discovery, lactate still remains an intriguing molecule. Considered for a long time as a waste product of metabolism and the culprit behind muscular fatigue, it was then recognized as an important fuel for many cells. In particular, in the nervous system, it has been proposed that lactate, released by astrocytes in response to neuronal activation, is taken up by neurons, oxidized to pyruvate and used for synthesizing acetyl-CoA to be used for the tricarboxylic acid cycle. More recently, in addition to this metabolic role, the discovery of a specific receptor prompted a reconsideration of its role, and lactate is now seen as a sort of hormone, even invol…

Human Memory Th17 Cell Populations Change Into Anti-inflammatory Cells With Regulatory Capacity Upon Exposure to Active Vitamin D

2019

Autoimmune diseases are characterized by an aberrantly activated immune system, resulting in tissue damage and functional disability in patients. An important therapeutic goal is to restore the deregulated immunological balance between pro- A nd anti-inflammatory T cells. This imbalance is illustrated by elevated levels and activity of memory Th17 cell populations, such as Th17, Th1/Th17, and Th17.1 cells, in various autoimmune diseases. These cells are characterized by the chemokine receptor CCR6, RORC expression and production of IL-17A, IFNγ, and TNFα. Using rheumatoid arthritis (RA) as a model of autoimmune disease, we here demonstrate that pro-inflammatory memory CCR6+ Th cells can swi…

Osteoclast Immunosuppressive Effects in Multiple Myeloma: Role of Programmed Cell Death Ligand 1

2018

Immunomodulatory drugs and monoclonal antibody-based immunotherapies have significantly improved the prognosis of the patients with multiple myeloma (MM) in the recent years. These new classes of reagents target malignant plasma cells (PCs) and further modulate the immune microenvironment, which prolongs anti-MM responses and may prevent tumor occurrence. Since MM remains an incurable cancer for most patients, there continues to be a need to identify new tumor target molecules and investigate alternative cellular approaches using gene therapeutic strategies and novel treatment mechanisms. Osteoclasts (OCs), as critical multi-nucleated large cells responsible for bone destruction in >80% …