Search results for "Repo"
showing 10 items of 2753 documents
Crystalline subtype of pre-descemetic corneal dystrophy.
2014
Purpose: To report corneal findings in a familial case of the crystalline subtype of pre- Descemetic corneal dystrophy. Case Report: A 19-year-old girl and her 44-year-old mother were found to have asymptomatic, bilateral, punctiform and multi-colored crystalline opacities across the whole posterior layer of the corneas. Endothelial specular microscopy revealed the presence of white round flecks located at different levels anterior to the endothelium. No systemic abnormalities or medications could be related to account for these findings. Conclusion: To the best of our knowledge, this is the third familial report of this rare corneal disorder. Differential diagnosis may include Schnyder cor…
La locura, ¿una respuesta literaria a la violencia en Colombia? En torno a 'Delirio' de Laura Restrepo
2014
Resumen : Delirio , la novela mas internacional de la colombiana Laura Restrepo, nos acerca, desde un angulo particular, a la realidad vivida en el pais durante la decada de los noventa. En ella somos testigos de como la violencia moldea a su antojo las vivencias de tres generaciones de personajes cuyo entramado de voces nos llegan bien en forma de susurros, bien vociferando sus consecuencias delirantes. Es por ello que nos disponemos a examinar en este trabajo, en que medida aparece la locura como respuesta a los males de la nacion, atendiendo especialmente al modo en que esto contribuye a la construccion de la memoria del yo. Abstract: Delirio, the most international novel of Colombian wr…
Particulated wisdom teeth as an autologous bone substitute for grafting/filling material in bone defects : case Report
2020
Introduction Bone augmentation material using permanent teeth are confirmed in many articles to provide good histological and clinical results. Advances in osteoconduction and osteoinduction, low cost of material, no risk of disease transmission and elimination of foreign body reaction are important aspects not only to the clinician but to patient as well. Many techniques and devices to obtain graft material for bone augmentation out of teeth are found in the literature. Material and Methods The wisdom teeth crush technique developed in our office and described in this case report is simple and no specific devices for teeth grinding were used. The graft material was obtained from impacted i…
Molecular basis of the functional distinction between Cln1 and Cln2 cyclins
2012
Cln1 and Cln2 are very similar but not identical cyclins. In this work, we tried to describe the molecular basis of the functional distinction between Cln1 and Cln2. We constructed chimeric cyclins containing different fragments of Cln1 and Cln2 and performed several functional analysis that make it possible to distinguish between Cln1 or Cln2. We identified that region between amino acids 225 and 299 of Cln2 is not only necessary but also sufficient to confer Cln2 specific functionality compared with Cln1. We also studied Cln1 and Cln2 subcellular localization identifying additional differences between them. Both cyclins are distributed between the nucleus and the cytoplasm, but Cln1 shows…
Amyloid Precursor-like Protein 1 Influences Endocytosis and Proteolytic Processing of the Amyloid Precursor Protein
2005
Ectodomain shedding of the amyloid precursor protein (APP) is a key regulatory step in the generation of the Alzheimer disease amyloid beta peptide (Abeta). The molecular mechanisms underlying the control of APP shedding remain little understood but are in part dependent on the low density lipoprotein receptor-related protein (LRP), which is involved in APP endocytosis. Here, we show that the APP homolog APLP1 (amyloid precursor-like protein 1) influences APP shedding. In human embryonic kidney 293 cells expression of APLP1 strongly activated APP shedding by alpha-secretase and slightly reduced beta-secretase cleavage. As revealed by domain deletion analysis, the increase in APP shedding re…
A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase
1999
Protein tyrosine phosphatase PTP-SL retains mitogen-activated protein (MAP) kinases in the cytoplasm in an inactive form by association through a kinase interaction motif (KIM) and tyrosine dephosphorylation. The related tyrosine phosphatases PTP-SL and STEP were phosphorylated by the cAMP-dependent protein kinase A (PKA). The PKA phosphorylation site on PTP-SL was identified as the Ser231 residue, located within the KIM. Upon phosphorylation of Ser231, PTP-SL binding and tyrosine dephosphorylation of the MAP kinases extracellular signal–regulated kinase (ERK)1/2 and p38α were impaired. Furthermore, treatment of COS-7 cells with PKA activators, or overexpression of the Cα catalytic subunit …
Adolescents' medicine use for headache: secular trends in 20 countries from 1986 to 2010
2015
BACKGROUND: This study reports secular trends in medicine use for headache among adolescents in 20 countries from 1986 to 2010.METHODS: The international Health Behaviour in School-aged Children (HBSC) survey includes self-reported data about medicine use for headaches among nationally representative samples of 11-, 13- and 15-year-olds. We included 20 countries with data from at least three data collection waves, with a total of 380 129 participants.RESULTS: The prevalence of medicine use for headaches varied from 16.5% among Hungarian boys in 1994 to 62.9% among girls in Wales in 1998. The prevalence was higher among girls than boys in every country and data collection year. The prevalenc…
EGFP Reporters for Direct and Sensitive Detection of Mutagenic Bypass of DNA Lesions
2020
The sustainment of replication and transcription of damaged DNA is essential for cell survival under genotoxic stress
Cockayne syndrome: varied requirement of transcription-coupled nucleotide excision repair for the removal of three structurally different adducts fro…
2014
Hereditary defects in the transcription-coupled nucleotide excision repair (TC-NER) pathway of damaged DNA cause severe neurodegenerative disease Cockayne syndrome (CS), however the origin and chemical nature of the underlying DNA damage had remained unknown. To find out, to which degree the structural properties of DNA lesions determine the extent of transcription arrest in human CS cells, we performed quantitative host cell reactivation analyses of expression vectors containing various synthetic adducts. We found that a single 3-(deoxyguanosin-N 2-yl)-2-acetylaminofluorene adduct (dG(N 2)-AAF) constitutes an unsurmountable obstacle to transcription in both CS-A and CS-B cells and is remov…
Excision of Uracil from Transcribed DNA Negatively Affects Gene Expression
2014
Uracil is an unavoidable aberrant base in DNA, the repair of which takes place by a highly efficient base excision repair mechanism. The removal of uracil from the genome requires a succession of intermediate products, including an abasic site and a single strand break, before the original DNA structure can be reconstituted. These repair intermediates are harmful for DNA replication and also interfere with transcription under cell-free conditions. However, their relevance for cellular transcription has not been proved. Here we investigated the influence of uracil incorporated into a reporter vector on gene expression in human cells. The expression constructs contained a single uracil opposi…