Search results for "Restriction"
showing 10 items of 527 documents
Mitochondrial and chromosomal DNA alterations in human chromophobe renal cell carcinomas
1992
Renal cell tumours are characterized by the loss of chromosome 3p and trisomy of 5q segments (common, non-papillary renal cell carcinoma), or by trisomy of chromosomes 7 and 17 and loss of the Y chromosome (papillary renal cell carcinoma), or by random karyotype changes and mitochondrial DNA alterations (renal oncocytoma). We have studied by means of RFLP analysis the genomic and mitochondrial DNA in 11 chromophobe renal cell carcinomas, which have a unique morphology among kidney cancers. We found a loss of the constitutional heterozygosity at chromosomal regions 3p, 5q, 17p, and 17q, a combination of allelic losses that has not been found in other types of renal cell tumours. Three of the…
An innovative way to highlight the power of each polymorphism on elite athletes phenotype expression
2017
The purpose of this study was to determine the probability of soccer players having the best genetic background that could increase performance, evaluating the polymorphism that are considered Performance Enhancing Polymorphism (PEPs) distributed on five genes: PPAR alpha, PPARGC1A, NRF2, ACE e CKMM. Particularly, we investigated how each polymorphism works directly or through another polymorphism to distinguish elite athletes from non-athletic population. Sixty professional soccer players (age 22.5 +/- 2.2) and sixty healthy volunteers (age 21.2 +/- 2.3) were enrolled. Samples of venous blood was used to prepare genomic DNA. The polymorphic sites were scanned using PCR-RFLP protocols with …
Could starvation minimize chemotherapy-induced toxicities?
2008
Background: In their recent paper Raffaghello, et al. examined the use of short-term starvation (STS) to induce differential stress resistance (DSR), that is increased protection of normal over cancer cells against chemotherapy-induced oxidative stress, using a range of model organisms. Objective/methods: We examine the results of this study and their significance. Results/conclusions: Raffaghello, et al. obtained evidence that STS induced DSR in yeast, mammalian cell cultures, and mice. It is possible that calorie restriction extends lifespan and prevents chronic diseases like tumors, by braking proliferation. We think that molecular mechanisms determining STS-induced DFS in mammals should…
Polyphasic taxonomy of a novel yeast isolated from antarctic environment; description of Cryptococcus victoriae sp. nov.
1999
In 1992 some samples of mosses, lichens and soils were collected from Botany Bay, Southern Victoria Land (77 degrees 01' S 162 degrees 32' E) and, as a result of a routine screening programme some yeasts were isolated. One of them, designated as strain G5, showed marked differences when compared to other antarctic yeasts. According to morphological and physiological characteristics, we were able to identify the strain G5 as a yeast belonging to the genus Cryptococcus. Some characteristics of this genus are the growth response to myo-inositol, celobiose, raffinose and D-glucuronate, no-fermentation, the absence of mycelium and pseudomycelium, asexual reproduction, Diazolium blue B test (DBB)…
Chloroplast DNA evidence for introgression and long distance dispersal in the desert annualSenecio flavus (Asteraceae)
1995
Phylogenetic analysis of chloroplast DNA (cpDNA) restriction site variation supports a close genetic relationship between the Southwest AsianSenecio flavus subsp.breviflorus and the North AmericanS. mohavensis. The intercontinental disjunct distribution of these two desert annuals may have originated via long distance dispersal. The chloroplast genomes of the Southern and North AfricanS. flavus subsp.flavus and subsp.breviflorus differ by at least ten restriction sites, while at most two restriction sites differentiate the cpDNA genomes of subsp.breviflorus and the outgroupS. squalidus. This suggests that the cpDNA genome ofS. flavus subsp.breviflorus may have resulted from introgression an…
Non-isotopic DNA fingerprint analyses with the minisatellite probe MZ1.3
1990
RFLP analyses with minisatellite probes yield highly informative individual specific banding patterns (genetic fingerprints) (Jeffreys et al.). Because of the complexity of the pattern and marked differences in band intensities a good band resolution and high sensitivity of the probe are essential. By reason of the latter requirement to date most of the RFLP-studies are performed with radioactive probes. Although non-isotopic labels have been introduced into fingerprint analyses (Schafer et al.; Medeiros et al.) 32P-labeled probes are still superior with respect to sensitivity. Our approach to increase the specific signal intensity makes use of an amplification of the number of probe molecu…
Detection of a Single Gene Encoding Glutamine Synthetase in Sinapis alba (L.)
1991
Summary Ion-exchange chromatography of glutamine synthetase polypeptides (GS; EC 6.3.1.2) from green leaves and the roots of Sinapis alba yielded identical elution patterns. This is likewise true for GS from etiolated cotyledons. As we have previously demonstrated the identity of GS-enzymes from etiolated and green leaf tissues, the obviously very similar charge properties of GS-proteins indicate the eventual existence of only one type of GS in all mustard plant organs. To further prove this possibility, Southern blot analysis of mustard DNA was carried out using hybridization probes specific to different GS-isoforms. Concluding from the relative strength of the hybridization signals, the G…
Prenatal Clinical Assessment of sFlt-1 (Soluble fms-like Tyrosine Kinase-1)/PlGF (Placental Growth Factor) Ratio as a Diagnostic Tool for Preeclampsi…
2013
Background: Aim of the study was a critical assessment of the clinical validity of the prenatal determination of sFlt-1/PlGF for preeclampsia (PE), pregnancy-induced hypertension (PIH), and proteinuria. Our analysis was based on a specificity of 95 % and a sensitivity of 82 % for the prediction of preeclampsia, as described by Elecsys (Roche). Methods: In this retrospective study the ratio of the prenatal antiangiogenic factor sFlt-1 (soluble fms-like tyrosine kinase-1) to the proangiogenic factor PIGF (placental growth factor) was analyzed using the electrochemiluminescence immunoassay of Elecsys (Roche Diagnostics, Mannheim, Germany) in 173 pregnant women. Sixty-three women with PE, 34 wo…
Fetal growth restriction: A growth pattern with fetal, neonatal and long-term consequences
2019
Fetal growth restriction (FGR) or intrauterine growth restriction (IUGR) are the terms used for a fetus which has not attained its full growth potential for gestational age. FGR is a multifactorial syndrome responsible for increased fetal and neonatal morbidity and mortality as well as long term adverse outcomes involving auxological, metabolic, organic and functional domains. Clinicians distinguish early and late onset FGR, in relation to specific fetal anthropometric parameters related to the possible primary etiology and to different patterns of placental and maternal cardiovascular pathologies. Delivery of an early onset FGR or growth impaired newborn with congenital pathology should be…
Postnatal programming cardiometabolic risk : effects of moderate caloric restriction in adulthood
2016
Postnatal overfeeding (PNOF) in rodents induces early programming of cardio-metabolic risk. Our aim was to determine if a moderate diet restriction could restore cardio-metabolic alterations induced by PNOF.Immediately after birth, litters of C57BL/6 mice were either maintained at 9 (normal litter, NL), or reduced to 3 (small litter, SL) to induce PNOF. At weaning, all mice received a standard diet ad libitum (AL). At 6 month of age, half of the NL and SL mice were assigned to a moderate 20% calorie restriction (CR: NLCR, SLCR) for one month, while the other mice continued to eat AL (AL: NLAL, SLAL). Glucose and insulin tolerance tests, cardiac function (echocardiography), body composition …