Search results for "Rhizophagus irregularis"
showing 7 items of 17 documents
Proteome adaptations under contrasting soil phosphate regimes of Rhizophagus irregularis engaged in a common mycorrhizal network.
2021
International audience; For many plants, their symbiosis with arbuscular mycorrhizal fungi plays a key role in the acquisition of mineral nutrients such as inorganic phosphate (Pi), in exchange for assimilated carbon. To study gene regulation and function in the symbiotic partners, we and others have used compartmented microcosms in which the extra-radical mycelium (ERM), responsible for mineral nutrient supply for the plants, was separated by fine nylon nets from the associated host roots and could be harvested and analysed in isolation. Here, we used such a model system to perform a quantitative comparative protein profiling of the ERM of Rhizophagus irregularis BEG75, forming a common my…
Influence of arbuscular mycorrhizal colonisation on cadmium induced Medicago truncatula root isoflavonoid accumulation.
2012
Cadmium is a serious environmental pollution threats to the planet. Its accumulation in plants affects many cellular functions, resulting in growth and development inhibition, whose mechanisms are not fully understood. However, some fungi forming arbuscular mycorrhizal symbiosis with the majority of plant species have the capacity to buffer the deleterious effect of this heavy metal. In the present work we investigated the capacity of Rhizophagus irregularis (syn. Glomus irregularis) to alleviate cadmium stress in Medicago truncatula. In spite of a reduction in all mycorrhizal parameters, plants colonized for 21 days by R. irregularis and treated by 2 mg kg(-1) cadmium displayed less growth…
Early screening of new accumulating versus non-accumulating tree species for the phytomanagement of marginal lands
2019
International audience; The use of fast-growing trees producing a high quantity of biomass can bring significant practical and economic benefits to the reclamation of marginal lands. The present study aims to identify new shrub/tree species to offer a wider range of shrubs/trees useful for phytomanagement practices. We implemented three experimental sites in France of 1 ha each (Thann, Carrières-sous-Poissy and Leforest) contaminated by different potentially toxic elements (PTE) with a total of 38 different tree species. After two years of growth, the element concentrations in stem and leaf biomasses, tree survival rate and growth of plants were assessed. Although the three sites had elevat…
Changements à court terme de la composition des communautés de champignons mycorhiziens à arbuscules et des populations de Rhizophagus irregularis en…
2014
La mycorhize à arbuscule a un important potentiel d’amélioration de la nutrition minérale des plantes, fort utile dans le contexte agricole. Cependant, certaines pratiques culturales sont connues pour avoir un effet néfaste sur la diversité des champignons mycorhiziens à arbuscules (Glomeromycètes). Il est donc nécessaire de mieux comprendre les dynamiques de leurs communautés et populations pour pouvoir préserver et gérer le potentiel symbiotique. Dans cette étude, nous avons étudié les effets séparés et conjoints du retournement du sol et des fertilisations phosphatée et azotée du sol sur la structure des communautés des Glomeromycètes et sur les populations de l’espèce Rhizophagus irregu…
The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis
2014
International audience; Arbuscular mycorrhizal (AM) symbiosis that associates roots of most land plants with soil-borne fungi (Glomeromycota), is characterized by reciprocal nutritional benefits. Fungal colonization of plant roots induces massive changes in cortical cells where the fungus differentiates an arbuscule, which drives proliferation of the plasma membrane. Despite the recognized importance of membrane proteins in sustaining AM symbiosis, the root microsomal proteome elicited upon mycorrhiza still remains to be explored. In this study, we first examined the qualitative composition of the root membrane proteome of Medicago truncatula after microsome enrichment and subsequent in dep…
Recherche de systèmes de transport de potassium impliqués dans le transfert de K+ de la mycorhize arbusculaire au riz lors d'interactions symbiotiques
2022
Les champignons mycorhiziens à arbuscules (CMA) développent des connexions interdépendantes avec les racines d'environ 90% des espèces végétales. Ces interactions augmentent la disponibilité ainsi que la translocation des nutriments (en particulier N et P), et améliorent ainsi la nutrition et la croissance des plantes. De plus, la résistance à une variété de stress, parmi lesquels le stress salin, s'est avérée améliorée par les interactions CMA-plante, par exemple chez le riz. Des recherches intenses pour expliquer les mécanismes moléculaires des interactions bénéfiques CMA-plante ont conduit à l'identification de transporteurs de phosphate et d'ammonium impliqués dans les échanges de nutri…
Label-free 1-DE-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins
2012
Deep changes in the shape and number of organelles, together with profound modifications in various membrane compartments, are induced within arbuscular mycorrhizal (AM) symbiosis. In this context, to investigate the membrane-associated proteins that are regulated in the model interaction Medicago truncatula – Rhizophagus irregularis, label-free 1DE-LC-MS/MS approach has been employed as alternative to two-dimensional gel electrophoresis. The existence of a correlation between protein abundance and peak areas or number of MS/MS spectra has widened the choice of label-free quantitative proteomics. The results highlighted microsomal protein candidates that could be involved in the symbiotic e…