Search results for "Rope"

showing 10 items of 10402 documents

Multilocus microsatellite analysis of European and African Candida glabrata isolates

2016

This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was …

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia ClinicaClonal complexEpidemiologyMultilocus microsatellite analysisCandida glabrataMolecular phylogenyGene locusCentral typeRelated genotypeGenotypeEnvironmental MicrobiologyHaplotypeDNA FungalPriority journalGeneticsAlleleCandidiasisGeneral MedicineClassificationEuropePhenotypeInfectious DiseasesCandida Glabrata; Adhesins; FluconazoleCandidiasiMicrosatelliteMicrosatellite RepeatMicrobiological examinationHumanMicrobiology (medical)GenotypeSettore MED/17 - Malattie InfettiveMicrosatellite DNA030106 microbiologyBiologyEuropeanMicrobiologyArticle03 medical and health sciencesGenetic variationMicrosatellite repeatsGeneticsHumansAlleleGenotypingAllelesScience & TechnologyCandida glabrataMicrosatellite markerHaplotypeAfricanGenetic Variationbiology.organism_classificationNonhuman030104 developmental biologyFungal DNAHaplotypesIsolation and purificationGenetic LociAfricaMultilocus sequence typingFungus isolationGenetic variabilityMicrosatellite genotypeMultilocus Sequence Typing
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Potential Uses of Olive Oil Secoiridoids for the Prevention and Treatment of Cancer: A Narrative Review of Preclinical Studies

2021

The Mediterranean diet (MD) is a combination of foods mainly rich in antioxidants and anti-inflammatory nutrients that have been shown to have many health-enhancing effects. Extra-virgin olive oil (EVOO) is an important component of the MD. The importance of EVOO can be attributed to phenolic compounds, represented by phenolic alcohols, hydroxytyrosol, and tyrosol, and to secoiridoids, which include oleocanthal, oleacein, oleuropein, and ligstroside (along with the aglycone and glycosidic derivatives of the latter two). Each secoiridoid has been studied and characterized, and their effects on human health have been documented by several studies. Secoiridoids have antioxidant, anti-inflammat…

0301 basic medicineSettore MED/09 - Medicina InternaSettore CHIM/10 - Chimica Degli AlimentiMediterranean dietAnti-Inflammatory AgentsReviewDiet MediterraneanAntioxidantsCyclopentane Monoterpeneslcsh:Chemistrychemistry.chemical_compound0302 clinical medicineGlucosidesNeoplasmsIridoidslcsh:QH301-705.5SpectroscopyTraditional medicineGeneral MedicinePhenylethyl AlcoholComputer Science Applications030220 oncology & carcinogenesissecoiridoidsIridoid GlucosidesAntineoplastic AgentsoleocanthalCatalysisInorganic Chemistry03 medical and health sciencesPhenolsOleuropeinOleocanthalmedicineAnimalsHumanscanceroleaceinPhysical and Theoretical ChemistryOlive OilMolecular BiologyPyransAldehydesOrganic ChemistryCancermedicine.diseaseTyrosol030104 developmental biologyAglyconelcsh:Biology (General)lcsh:QD1-999chemistryoleuropeinligstrosideHydroxytyrosolOlive oilInternational Journal of Molecular Sciences
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Retrieving infinite numbers of patterns in a spin-glass model of immune networks

2013

The similarity between neural and immune networks has been known for decades, but so far we did not understand the mechanism that allows the immune system, unlike associative neural networks, to recall and execute a large number of memorized defense strategies {\em in parallel}. The explanation turns out to lie in the network topology. Neurons interact typically with a large number of other neurons, whereas interactions among lymphocytes in immune networks are very specific, and described by graphs with finite connectivity. In this paper we use replica techniques to solve a statistical mechanical immune network model with `coordinator branches' (T-cells) and `effector branches' (B-cells), a…

0301 basic medicineSimilarity (geometry)Spin glassComputer sciencestatistical mechanicFOS: Physical sciencesGeneral Physics and AstronomyNetwork topologyTopology01 natural sciencesQuantitative Biology::Cell Behavior03 medical and health sciencesCell Behavior (q-bio.CB)0103 physical sciencesattractor neural-networks; statistical mechanics; brain networks; Physics and Astronomy (all)Physics - Biological Physics010306 general physicsAssociative propertybrain networkArtificial neural networkMechanism (biology)ErgodicityDisordered Systems and Neural Networks (cond-mat.dis-nn)Condensed Matter - Disordered Systems and Neural NetworksAcquired immune system030104 developmental biologyBiological Physics (physics.bio-ph)FOS: Biological sciencesattractor neural-networkQuantitative Biology - Cell Behavior
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Corrigendum to “European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS…

2018

The European Cooperation in Science and Technology (COST) provides an ideal framework to establish multi-disciplinary research networks. COST Action BM1203 (EU-ROS) represents a consortium of researchers from different disciplines who are dedicated to providing new insights and tools for better understanding redox biology and medicine and, in the long run, to finding new therapeutic strategies to target dysregulated redox processes in various diseases. This report highlights the major achievements of EU-ROS as well as research updates and new perspectives arising from its members. The EU-ROS consortium comprised more than 140 active members who worked together for four years on the topics b…

0301 basic medicineSocieties ScientificRedox signalingInternational CooperationClinical BiochemistryNanotechnologyReview ArticleBiologyPublic administrationBiochemistryAntioxidantsArticle03 medical and health sciencesmedia_common.cataloged_instanceAnimalsHumansCost actionEuropean UnionEuropean unionMolecular Biologylcsh:QH301-705.5media_commonFunding AgencyRedox therapeuticslcsh:R5-920Organic ChemistryReactive nitrogen species030104 developmental biologyWork (electrical)lcsh:Biology (General)Oxidative stressReactive Oxygen Specieslcsh:Medicine (General)Oxidation-ReductionSignal TransductionRedox Biology
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Sorafenib plus topotecan versus placebo plus topotecan for platinum-resistant ovarian cancer (TRIAS): a multicentre, randomised, double-blind, placeb…

2018

Summary Background Antiangiogenic therapy has known activity in ovarian cancer. The investigator-initiated randomised phase 2 TRIAS trial assessed the multi-kinase inhibitor sorafenib combined with topotecan and continued as maintenance therapy for platinum-resistant or platinum-refractory ovarian cancer. Methods We did a multicentre, double-blind, placebo-controlled, randomised, phase 2 trial at 20 sites in Germany. Patients (≥18 years) with platinum-resistant ovarian cancer previously treated with two or fewer chemotherapy lines for recurrent disease were stratified (first vs later relapse) in block sizes of four and randomly assigned (1:1) using a web-generated response system to topotec…

0301 basic medicineSorafenibAdultmedicine.medical_specialtyTime FactorsPerforation (oil well)Angiogenesis InhibitorsPlatinum CompoundsNeutropeniaPlaceboGastroenterologyDrug Administration Schedule03 medical and health sciences0302 clinical medicineMaintenance therapyDouble-Blind MethodInternal medicineGermanyAntineoplastic Combined Chemotherapy ProtocolsmedicineClinical endpointHumansProgression-free survivalProtein Kinase InhibitorsAgedOvarian Neoplasmsbusiness.industryMiddle AgedSorafenibmedicine.diseaseProgression-Free Survival030104 developmental biologyOncologyDrug Resistance Neoplasm030220 oncology & carcinogenesisDisease ProgressionTopotecanFemaleTopoisomerase I InhibitorsbusinessTopotecanmedicine.drugThe Lancet. Oncology
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Assessing statistical significance in multivariable genome wide association analysis

2016

Motivation: Although Genome Wide Association Studies (GWAS) genotype a very large number of single nucleotide polymorphisms (SNPs), the data are often analyzed one SNP at a time. The low predictive power of single SNPs, coupled with the high significance threshold needed to correct for multiple testing, greatly decreases the power of GWAS. Results: We propose a procedure in which all the SNPs are analyzed in a multiple generalized linear model, and we show its use for extremely high-dimensional datasets. Our method yields P-values for assessing significance of single SNPs or groups of SNPs while controlling for all other SNPs and the family wise error rate (FWER). Thus, our method tests whe…

0301 basic medicineStatistics and Probability1303 BiochemistryGenotypeOperations researchLibrary sciencePolymorphism Single NucleotideBiochemistryGerman03 medical and health sciences10007 Department of EconomicsPolitical scienceGenome-Wide Association Analysis1312 Molecular Biology1706 Computer Science ApplicationsCluster AnalysisHumansComputer Simulation2613 Statistics and ProbabilityMolecular BiologyEuropean researchGenetics and Population AnalysisComputational BiologyReproducibility of ResultsOriginal Paperslanguage.human_languageComputer Science Applications330 EconomicsComputational MathematicsPhenotype030104 developmental biologyComputational Theory and MathematicsLinear Modelslanguage2605 Computational MathematicsGenome-Wide Association Study1703 Computational Theory and Mathematics
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FLYCOP: metabolic modeling-based analysis and engineering microbial communities

2018

10 p.-5 fig.-2 tab.

0301 basic medicineStatistics and ProbabilityComputer scienceMetaboliteAuxotrophy030106 microbiologyMicrobial ConsortiaEccb 2018: European Conference on Computational Biology ProceedingsEvolutionary engineeringmedicine.disease_causeBiochemistry03 medical and health scienceschemistry.chemical_compoundmedicineEscherichia coliMetabolic modelingMolecular BiologyEscherichia coli2. Zero hungerbiologyMicrobiotaSystemsBiological evolutionSynechococcusbiology.organism_classificationComputer Science ApplicationsComputational MathematicsMulticellular organism030104 developmental biologyComputational Theory and MathematicschemistryMetabolic EngineeringBiochemical engineeringSoftwareBioinformatics
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Importance of stem cell culture conditions for their derived extracellular vesicles therapeutic effect.

2021

Stem cell-derived extracellular vesicles (EVs) could be enhanced by modifying specific in vitro parameters when culturing their originating stem cells. Controlling stem cell growth conditions with physical properties, oxygen tension and media preconditioning with soluble factors may influence EVs biogenesis and EVs biological function as well. Unfortunately, many misconceptions and methodological issues have hampered the progress in understanding the biological properties of EVs. In this review we will first discuss the major concerns involved in a suitable EVs production from stem cell culture. Then, we will describe the current techniques for EV isolation, focusing on their advantages and…

0301 basic medicineStem CellsCell Culture TechniquesReproducibility of ResultsMesenchymal Stem CellsBiologyStem cell cultureBiochemistryExtracellular vesiclesOxygen tensionCell biology03 medical and health sciencesExtracellular Vesicles030104 developmental biology0302 clinical medicinePhysiology (medical)Biological propertyStem cell030217 neurology & neurosurgeryFree radical biologymedicine
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Ancient pigs reveal a near-complete genomic turnover following their introduction to Europe

2019

International audience; Archaeological evidence indicates that pig domestication had begun by ∼10,500 y before the present (BP) in the Near East, and mitochondrial DNA (mtDNA) suggests that pigs arrived in Europe alongside farmers ∼8,500 y BP. A few thousand years after the introduction of Near Eastern pigs into Europe, however, their characteristic mtDNA signature disappeared and was replaced by haplotypes associated with European wild boars. This turnover could be accounted for by substantial gene flow from local Euro-pean wild boars, although it is also possible that European wild boars were domesticated independently without any genetic contribution from the Near East. To test these hyp…

0301 basic medicineSwine[SHS.ANTHRO-BIO]Humanities and Social Sciences/Biological anthropologySkin Pigmentation[SHS]Humanities and Social SciencesGene flowDomesticationddc:590BREEDSDOMESTIC PIGS/dk/atira/pure/subjectarea/asjc/1000HISTORY0601 history and archaeologyNeolithicHistory AncientPhylogenyMultidisciplinary060102 archaeologyINTROGRESSIONEurope ; pigs ; domestication ; genomesWILD06 humanities and the artsArchaeological evidenceGene flowEuropeSPREADCoatMitochondrial DNAEvolutionZoology930Locus (genetics)BiologyAnimal Breeding and GenomicsDNA MitochondrialMiddle East03 medical and health sciencesAnimalsFokkerij en GenomicaDNA AncientGeneralDomesticationddc:930HaplotypeDNA900 Geschichte und Geografie::930 Geschichte des Altertums (bis ca. 499) Archäologie::930 Geschichte des Altertums bis ca. 499 ArchäologieLONGSIZE030104 developmental biologydomestication evolution gene flow NeolithicWIAS
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Phenolic Compounds in Extra Virgin Olive Oil Stimulate Human Osteoblastic Cell Proliferation.

2016

In this study, we aimed to clarify the effects of phenolic compounds and extracts from different extra virgin olive oil (EVOO) varieties obtained from fruits of different ripening stages on osteoblast cells (MG-63) proliferation. Cell proliferation was increased by hydroxytyrosol, luteolin, apigenin, p-coumaric, caffeic, and ferulic acids by approximately 11-16%, as compared with controls that were treated with one vehicle alone, while (+)-pinoresinol, oleuropein, sinapic, vanillic acid and derivative (vanillin) did not affect cell proliferation. All phenolic extracts stimulated MG-63 cell growth, and they induced higher cell proliferation rates than individual compounds. The most effective…

0301 basic medicineTime Factorslcsh:MedicineBiochemistryMass SpectrometryTreeschemistry.chemical_compoundAnimal CellsPlant ProductsMedicine and Health SciencesCaffeic acidApigeninlcsh:ScienceLuteolinChromatography High Pressure LiquidConnective Tissue CellsCultured Tumor CellsPrincipal Component AnalysisMultidisciplinaryAgricultureCell DifferentiationRipeningPlantsPhenylethyl AlcoholLipidsOsteoblast DifferentiationChemistryBiochemistryCell ProcessesConnective TissuePhysical SciencesApigeninBiological CulturesCellular TypesAnatomyResearch ArticleOlive TreesCoumaric AcidsResearch and Analysis MethodsVegetable Oils03 medical and health sciencesCaffeic AcidsPhenolsOleuropeinCell Line TumorOleaVanillic acidHumansPhenolsOlive OilCell ProliferationAnalysis of Variance030109 nutrition & dieteticsOsteoblastsDose-Response Relationship Druglcsh:RChemical CompoundsOrganismsBiology and Life SciencesCell BiologyCell CulturesOsteosarcoma CellsAgronomyOlive treesBiological Tissue030104 developmental biologychemistryFruitHydroxytyrosollcsh:QOilsCrop ScienceDevelopmental Biology
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