Search results for "SEPARATION"

Advances in blind source separation for spatial data

2021

Viele Datensaetze bestehen aus multivariaten Messungen, die an verschiedenen geographischen Orten durchgefuehrt wurden. Typischerweise besitzen solche Datensaetze die Eigenschaft, dass Messungen in unmittelbarer Naehe aehnlicher sind als Messungen, die eine hohe Entfernung aufweisen. In der statistischen Analyse solcher raeumlichen Daten sollte diese spezielle Eigenschaft beruecksichtigt werden. In letzter Zeit wurde in der statistischen Literatur die sogenannte Blind Source Separation Methode auf raeumliche Daten erweitert. In diesem Model wird angenommen, dass die Daten aus Linearkombinationen von unbeobachteten Variablen bestehen, und das Ziel ist diese latenten Variablen zu bestimmen. D…

IS IT IMPORTANT TO SEPARATE LEUCOCYTES AND PLATELETS BEFORE MEASURING THE FILTERABILITY OF RED BLOOD CELLS?

1985

Activation of cGMP-dependent Protein Kinase Iβ Inhibits Interleukin 2 Release and Proliferation of T Cell Receptor-stimulated Human Peripheral T Cells

2000

Several major functions of type I cGMP-dependent protein kinase (cGK I) have been established in smooth muscle cells, platelets, endothelial cells, and cardiac myocytes. Here we demonstrate that cGK Ibeta is endogenously expressed in freshly purified human peripheral blood T lymphocytes and inhibits their proliferation and interleukin 2 release. Incubation of human T cells with the NO donor, sodium nitroprusside, or the membrane-permeant cGMP analogs PET-cGMP and 8-pCPT-cGMP, activated cGK I and produced (i) a distinct pattern of phosphorylation of vasodilator-stimulated phosphoprotein, (ii) stimulation of the mitogen-activated protein kinases ERK1/2 and p38 kinase, and, upon anti-CD3 stimu…

Unsteady Separation and Navier-Stokes Solutions at High Reynolds Numbers

2010

We compute the numerical solutions for Navier-Stokes and Prandtl’s equations in the case of a uniform bidimensional flow past an impulsively started disk. The numerical approx- imation is based on a spectral methods imple- mented in a Grid environment. We investigate the relationship between the phenomena of unsteady separation of the flow and the exponential decay of the Fourier spectrum of the solutions. We show that Prandtl’s solution develops a separation singularity in a finite time. Navier-Stokes solutions are computed over a range of Reynolds numbers from 3000 to 50000. We show that the appearance of large gradients of the pressure in the stream- wise direction, reveals that the visc…

Three-Dimensional Calibration for Routine Analyses of Bromide and Nitrate Ions as Indicators of Groundwater Quality in Coastal Territories

2019

Nitrate and bromide ions are generally considered indicators of anthropogenic pollution and seawater intrusion, respectively, in the groundwater of coastal territories. The analysis of these species is generally carried out with routine chromatographic analyses which generally afford partially merged or poorly resolved peaks. In the present paper a simple method for the correct evaluation of their concentration in water is reported. This method does not imply utilization of other instruments or technologies, only the mathematical elaboration of the data obtained from routine analysis of standard solutions containing the two species. Standard binary solutions of nitrate and bromide ions at d…

Gelchromatographie. 6. Mitt. trennungen im präparativen maßstab mit hoher auflösung

1968

Zur gelchromatographischen Trennung im Grammengen-Bereich wurde ein Recycling-Verfahren mit kontinuierlicher Einengung des Eluates entwickelt. Die Trennung von Oligomeren des Styrols, des Butylmethacrylates und des Athylenoxids unter Anwendung dieser Methode wire beschrieben. Vorteile und Grenzen des Verfahrens werden diskutiert. A recycling technic with continuous concentration of the eluate has been developed for gel chromatographic separation in the gram-scale. The separation of oligomers of styrene, butyl methacrylate and ethylene oxide by use of this method is described. Advantages and limitation of this procedure are discussed.

Design methodology for submerged anaerobic membrane bioreactors (AnMBR): A case study

2015

[EN] The main objective of this study is to propose guidelines for designing submerged anaerobic MBR (AnMBR) technology for municipal wastewater treatment. The design methodology was devised on the basis of simulation and experimental results from an AnMBR plant featuring industrial-scale hollow-fibre membranes. The proposed methodology aims to minimise both capital expenditure and operating expenses, and the key parameters considered were: hydraulic retention time, solids retention time, mixed liquor suspended solids concentration in the membrane tank, 20 C-standardised critical flux, specificgas demand per square metre of membrane area, and flow of sludge being recycled from the membrane …

Immunelectronmicroscopic characterization of T4 and T8 lymphocytes and natural killer cells in neuronal ceroid-lipofuscinosis.

1995

CD4+, CD8+, and CD56+ cells were isolated with the immunomagnetic separation technique from peripheral blood mononuclear cells (PBMC) of 3 patients with neuronal ceroid-lipofuscinosis : one patient each with infantile (INCL), late infantile (LINCL), and juvenile (JNCL) neuronal ceroid-lipofuscinoses, all studied by light (LM) and electron (EM) microscopy. To compare the pathology of these cells with affected cells in other types of lysosomal diseases, the separation was also performed with PBMC of 1 patient with mucolipidosis (ML) type II, 2 patients with mucopolysaccharidosis (MPS) type I, and 4 patients with MPS type III. Disease-specific lysosomal inclusions were identified in CD4+, CD8+…

Isolation of CD4+ T cells from murine lungs: a method to analyze ongoing immune responses in the lung.

2007

The regulation of the cellular immune response in lung diseases is not yet fully understood. Isolating different subsets of immune cells directly from the lung is therefore an indispensable method of gaining detailed knowledge on the function of these cells in this organ. This protocol describes a method of isolating and magnetically labeling CD4+ lung T cells, which are then loaded and retained on the column while all other cells run through it (positive selection). The yield of this isolation is approximately 5 x 10(5) to 1.5 x 10(6) CD4+ cells from a murine lung. These cells can be further investigated by several methods such as flow cytometry, western blot analysis, RT-PCR, immunostaini…

In vitro model for the activation of CD4 and CD8 T cell receptors.

2008

Previously, most models that sought to explain the misregulation of immune cell function assumed molecular similarities between the disease-causing pathogens and the host's proteins. In recent time several different models have been proposed and in this study, these concepts are compared to a new hypothesis proposing another explanation for this immune dysregulation: the possibility that the mislocalization of proteins may be responsible for autoimmune activity. Based on this hypothesis, proteins are recognized as self or non-self depending on where they appear in sufficiently high concentrations. To examine this new idea, the intracellular human proteins beta-actin, GAPDH, and hemoglobin a…