Search results for "SEPARATION"
showing 10 items of 865 documents
The Relevance of Flow Cytometry for Biochemical Analysis
2001
Flow cytometry (FCM) allows the simultaneous measurement of multiple fluorescences and light scatter induced by illumination of single cells or microscopic particles in suspension, as they flow rapidly through a sensing area. In some systems, individual cells or particles may be sorted according to the properties exhibited. By using appropriate fluorescent markers, FCM is unique in that multiple structural and functional parameters can be quantified simultaneously on a single-particle basis, whereas up to thousands of biological particles per second may be examined. FCM is increasingly used for basic, clinical, biotechnological, and environmental studies of biochemical relevance. In this cr…
Bioelectric model of atrial fibrillation: Applicability of blind source separation techniques for atrial activity estimation in atrial fibrillation e…
2003
In this contribution, we present the theoretical justification that give support to the suitability of blind signal separation (BSS) techniques for the estimation of the atrial activity (AA) present in ECGs of persistent atrial fibrillation (AF). The application of BSS methods to this problem needs the fulfillment of several conditions regarding AA, ventricular activity (VA) and the fashion in which both activities arise on the body surface, that will be justified along the paper. To empirically validate the model, an ICA method is applied to 10 real 12-lead recordings of AF. The identification of AA is put forward based on kurtosis and spectral analysis. The kurtosis value of the estimated…
Analysis and review of the perioperative management of COVID-19 patients in thoracic surgery.
2021
Resumen Debido a la grave situacion de pandemia que vivimos causada por el COVID-19, la Seccion de Anestesia Cardiaca, Vascular y Toracica de la SEDAR pretende dar informacion de los aspectos mas relevantes a considerar en pacientes con sospecha o infeccion confirmada por coronavirus que van a ser sometidos a cirugia toracica. Creemos que es esencial mantener una optima transmision de informacion para llevar a cabo maniobras seguras, tanto para los pacientes como para el personal sanitario. La estrategia de manejo debe basarse en una organizacion exquisita del trabajo, definiendo quien, como y cuando va a realizar cada tarea y procedimiento. Las recomendaciones que se proponen, tras valorar…
Thoracic Anesthesia during the 2019 Novel Coronavirus Infection Pandemic: 2021 Updated Recommendations for Airway Management by the EACTAIC Thoracic …
2021
Contains fulltext : 244115.pdf (Publisher’s version ) (Closed access) The novel coronavirus pandemic has radically changed the landscape of normal surgical practice. Lifesaving cancer surgery, however, remains a clinical priority, and there is an increasing need to fully define the optimal oncologic management of patients with varying stages of lung cancer, allowing prioritization of which thoracic procedures should be performed in the current era. Healthcare providers and managers should not ignore the risk of a bimodal peak of mortality in patients with lung cancer; an imminent spike due to mortality from acute coronavirus disease 2019 (COVID-19) infection, and a secondary peak reflecting…
Small for gestational age (SGA) neonates show reduced suppressive activity of their regulatory T cells
2009
Little information exists concerning the role of fetal regulatory T cells (Tregs) during intrauterine development. We examined whether complications such as reduced birth weight or the occurrence of preterm labor were associated with deficiencies in the number or in the immunosuppressive activity of Tregs in the fetal circulation. Their total number did not change during normal or complicated pregnancy. In contrast, their level of FoxP3 expression decreased continuously with gestational age and was significantly reduced in the presence of spontaneous term, but not preterm labor. In small for gestational age (SGA) neonates, FoxP3 expression was constantly decreased when compared to age match…
Attachment styles in children affected by migraine without aura
2013
Maria Esposito,1 Lucia Parisi,2 Beatrice Gallai,3 Rosa Marotta,4 Anna Di Dona,1 Serena Marianna Lavano,2 Michele Roccella,4 Marco Carotenuto11Center for Childhood Headache, Clinic of Child and Adolescent Neuropsychiatry, Department of Mental Health, Physical and Preventive Medicine, Second University of Naples, Naples, Italy; 2Child Neuropsychiatry, Department of Psychology, University of Palermo, Palermo, Italy; 3Unit of Child and Adolescent Neuropsychiatry, University of Perugia, Perugia, Italy; 4Department of Psychiatry, The Magna Graecia University of Catanzaro, Catanzaro, ItalyBackground: In recent years, great attention has been given to the presence of psychological problems and psyc…
SUBFRACTIONS AND SUBPOPULATIONS OF HDL: AN UPDATE
2014
High-density lipoproteins (HDL) are classified as atheroprotective because they are involved in transport of cholesterol to the liver, known as "reverse cholesterol transport (RCT)" exerting antioxidant and anti-inflammatory activities. There is also evidence for cytoprotective, vasodilatory, antithrombotic, and anti-infectious activities for these lipoproteins. HDLs are known by structural, metabolic and biologic heterogeneity. Thus, different methods are able to distinguish several subclasses of HDL. Different separation techniques appear to support different HDL fractions as being atheroprotective or related with lower cardiovascular (CV) risk. However, HDL particles are not always prote…
Human antiphospholipid antibodies induce TNFα in monocytes via Toll-like receptor 8
2009
The antiphospholipid syndrome (APS) is characterized by recurrent arterial and/or venous thromboses, pregnancy loss and the presence of antiphospholipid antibodies (aPL). One of the discussed mechanisms of this thrombotic activity in APS patients is attributed to TNFalpha secretion in monocytes after aPL stimulation. To investigate this mechanism in detail, we employed a monoclonal aPL and IgG fractions of APS patients for stimulation of human peripheral monocytes. Stimulation with this monoclonal aPL resulted in an increased expression and secretion of TNFalpha, caused by specific upregulation of TLR8 mRNA and protein expression levels. To confirm the specificity of this finding we could d…
Occurrence of Microplastics in Waste Sludge of Wastewater Treatment Plants: Comparison between Membrane Bioreactor (MBR) and Conventional Activated S…
2022
In this study, the presence of microplastics in the sludge of three wastewater treatment plants (WWTPs) was examined. The investigated WWTPs operated based on a conventional activated sludge (CAS) process, with (W1) or without (W2) primary clarification, and a membrane bioreactor process (MBR) (W3). The microplastics (MPs) concentration in the samples of W3 was approximately 81.1 ± 4.2 × 103 particles/kg dry sludge, whereas MPs concentrations in W1 and W2 were 46.0 ± 14.8 × 103 particles/kg dry sludge and 36.0 ± 5.2 × 103 particles/kg dry sludge, respectively. Moreover, MPs mainly consisted of fragments (66–68%) in the CAS plants, whereas the fra…
Isolation and Culture of Oligodendrocytes
2019
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.