Search results for "SERT"
showing 10 items of 826 documents
Assembly of Spinach Chloroplast ATP Synthase Rotor Ring Protein-Lipid Complex
2019
Rotor ATPases are large multisubunit membrane protein complexes found in all kingdoms of life. The membrane parts of these ATPases include a ring-like assembly, so-called c-ring, consisting of several subunits c, plugged by a patch of phospholipids. In this report, we use a nature-inspired approach to model the assembly of the spinach (Spinacia oleracea) c14 ring protein-lipid complex, where partially assembled oligomers are pulled toward each other using a biasing potential. The resulting assemblies contain 23 to 26 encapsulated plug lipids, general position of which corresponds well to experimental maps. However, best fit to experimental data is achieved with 15 to 17 lipids inside the c-…
panISa: ab initio detection of insertion sequences in bacterial genomes from short read sequence data.
2018
Abstract Motivation The advent of next-generation sequencing has boosted the analysis of bacterial genome evolution. Insertion sequence (IS) elements play a key role in prokaryotic genome organization and evolution, but their repetitions in genomes complicate their detection from short-read data. Results PanISa is a software pipeline that identifies IS insertions ab initio in bacterial genomes from short-read data. It is a highly sensitive and precise tool based on the detection of read-mapping patterns at the insertion site. PanISa performs better than existing IS detection systems as it is based on a database-free approach. We applied it to a high-risk clone lineage of the pathogenic spec…
VISMapper: ultra-fast exhaustive cartography of viral insertion sites for gene therapy
2017
The possibility of integrating viral vectors to become a persistent part of the host genome makes them a crucial element of clinical gene therapy. However, viral integration has associated risks, such as the unintentional activation of oncogenes that can result in cancer. Therefore, the analysis of integration sites of retroviral vectors is a crucial step in developing safer vectors for therapeutic use. Here we present VISMapper, a vector integration site analysis web server, to analyze next-generation sequencing data for retroviral vector integration sites. VISMapper can be found at: http://vismapper.babelomics.org . Because it uses novel mapping algorithms VISMapper is remarkably faster t…
Primjena ekstrakta proteina sirutke u proizvodnji smrznutog mliječnog deserta
2018
The aim of this work was to substantiate the use of whey protein extract (WPE), a by-product of dairy industry, combined with different berry purees in the formulation of a whipped frozen dairy dessert (WFDD). After freezing, the swelling ability of WPE decreased significantly while at higher temperatures of the dispersion medium, the degree of swelling increased. A mixture containing 55±2 % WPE and 45±2 % milk presented a homogeneous consistency while the further processing did not improve the structure of the mixture. Final formulations of WFDD containing different berry purees (i.e. 10 % black¬currant puree, 25 % cherry puree, or 30 % strawberry puree) were proposed for which the nutriti…
CHK1-targeted therapy to deplete DNA replication-stressed, p53-deficient, hyperdiploid colorectal cancer stem cells.
2017
ObjectiveCancer stem cells (CSCs) are responsible for tumour formation and spreading, and their targeting is required for tumour eradication. There are limited therapeutic options for advanced colorectal cancer (CRC), particularly for tumours carrying RAS-activating mutations. The aim of this study was to identify novel CSC-targeting strategies.DesignTo discover potential therapeutics to be clinically investigated as single agent, we performed a screening with a panel of FDA-approved or investigational drugs on primary CRC cells enriched for CSCs (CRC-SCs) isolated from 27 patients. Candidate predictive biomarkers of efficacy were identified by integrating genomic, reverse-phase protein mic…
Études scientifiques et marché du travail : éléments de réflexion sur la crise des formations en sciences
2007
Student disaffection towards sciences, most evident in pure science undergraduates, is causing increasing concern for the future of scientific training in France. If access to employment deteriorates more for science graduates than for graduates in other disciplines, science graduates will have a distinct advantage in terms of earnings and employment status, when the economic situation becomes less favourable. Within sciences, graduates of pure sciences have a more difficult transition from education to employment than those from applied sciences. Yet graduates from both disciplines compete for the same job in the private sector with pure science graduates receiving lower wages. The “crisis…
Le sentiment de discrimination des descendants d'immigrés : reflet d'une orientation contrariée et d'un chômage persistant
2012
National audience; La discrimination est analysée ici en croisant les perceptions des jeunes avec la réalité de leur insertion sur le marché du travail et de leur parcours d'éducation. Une orientation contrariée en fin de troisième a un impact sur les trajectoires ultérieures, comme l'obtention d'un emploi. Certes, le chômage est lié au niveau d'études, mais les descendants d'immigrés originaires du Maghreb ou d'Afrique subsaharienne y sont surexposés, mettant en relief une discrimination à l'embauche, discrimination vivement ressentie par les jeunes eux-mêmes.
A Tool to Assess Architectural Education from the Sustainable Development Perspective and the Students' Viewpoint
2021
[EN] Architectural education plays a fundamental role in achieving sustainable development by training future professionals who can contribute to sustainability through their practice. Nevertheless, to ensure the introduction of sustainable development in the education of future architects, it is fundamental to understand what is being done and what could be improved. Despite this, a big gap has been detected in the assessment of architectural education in Spain and worldwide. Thus, a close-ended questionnaire for students has been designed based on the outcomes from a literature review, exploratory interviews with specialists, and the qualitative analysis of two schools of architecture. Ad…
Retrieving Monitoring and Accounting Information from Constrained Devices in Internet-of-Things Applications
2013
Part 6: Monitoring Mechanisms; International audience; Internet-of-Things (IoT) is envisioned to provide connectivity to a vast number of sensing or actuating devices with limited computational and communication capabilities. For the organizations that manage these constrained devices, the monitoring of each device’s operational status and performance level as well as the accounting of their resource usage are of great importance. However, monitoring and accounting support is lacking in today’s IoT platforms. Hence, this paper studies the applicability of the Constrained Application Protocol (CoAP), a lightweight transfer protocol under development by IETF, for efficiently retrieving monito…