Search results for "SOM"
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Cilvēka 14. hromosomas proteasomu gēnu polimorfismu asociāciju ar multiplo sklerozi izpēte Latvijas populācijā
2018
Proteasomālo gēnu polimorfismi ir autoimūnu slimību riska faktori. Lai novērtētu potenciālus multiplās sklerozes (MS) biomarķierus Latvijas populācijā, veicām proteasomālo gēnu PSMA6 un PSMC6 polimorfismu genotipēšanu 280 pacientiem ar MS diagnozi, ko salīdzinājām ar iepriekš ievāktiem datiem no 305 kontrolēm ar mērķi izpētīt iespējamo asociāciju starp šo lokusu polimorfismiem un MS. Tālākā analīzē kombinācijā ar iepriekšējiem PSMA3 polimorfismu genotipēšanas datiem atradām divus klīniski nozīmīgus multi-lokusu genotipus un trīs klīniski nozīmīgus haplotipus. Daļai pētīto polimorfismu novērojām no alēlēm atkarīgas alternatīvas DNS sekundārās struktūras. PSMA6 rs1048990 polimorfisma minorajā…
Kurzemes iedzīvotāju un skandināvu kontakti vēlajā dzelzs laikmetā
2017
Maģistra darbā risināts jautājums par kuršu un Ziemeļkurzemes Baltijas somu sakariem ar skandināviem aizvēstures beigās. 1. nodaļa veltīta Kurzemes ģeogrāfijai un apdzīvotībai dzelzs laikmetā. 2. nodaļā apkopoti līdzšinējie sakaru pētījumi bronzas un vidējā dzelzs laikmeta kontekstā. 3. nodaļā analizēti rakstītie avoti par skandināvu aktivitātēm Kurzemē un pretēji – kuršu uzbrukumiem skandināvu zemēm. 4. nodaļā Kurzemes arheoloģiskais materiāls izmantots aktīvāko tirdzniecisko reģionu, importa priekšmetu izcelsmes vietu un hronoloģijas noteikšanai. Pētījuma gaitā secināts, ka rakstītie avoti koncentrējas uz zviedru un dāņu militārajām aktivitātēm Kurzemē, turpretīm arheoloģiskais materiāls …
The activation of aggresomal pathway in Coxsackievirus B3 infection
2016
Aggresomin muodostuminen on yksi solun puolustusmekanismeista, joka pyrkii estämään proteiinien haitallisen aggregoitumisen solulimassa. Aikaisemmat kokeet ovat näyttäneet, että ihmisen enterovirus-infektio aiheuttaa muutoksia vimentiinissä, tyypin III välikokoisessa filamentissa (Turkki et al., julkaisematon data). Nämä muutokset ovat hyvin samankaltaisia aggresomin muodostumisen aikana tapahtuvien muutoksien kanssa. Tämän tutkimuksen tarkoituksena oli selvittää mahdollisen aggresomin muodostumisreitin aktivoituminen Coxsackievirus B3 (CVB3) -infektiossa, ja hypoteesimme oli, että häkkimäisten vimentiinirakenteiden muodostumisen lisäksi myös muita aggresomin muodostumisreitin aktivoitumise…
Transfection of lipoma cells with papilloma bovine virus subgenomic fragment.
1991
Abstract Lipoma cells with consistent chromosomal aberration have been transfected with plasmids carrying papilloma bovine virus subgenomic fragment (PBV 69). The succesful transformation of the cells was ascerted on the changed growth pattern of the cells in liquid medium, colony formation in soft agar and modified cell appearrance in electron microscopy; transfection with PBV 69 has not been, however, sufficient to immortalize lipoma cells.
Internalization of novel non-viral vector TAT-streptavidin into human cells
2007
BMC Biotechnology, 7 (1)
Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane
2021
Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although …
Binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells
1995
Infection of cells by human papillomaviruses (HPVs) associated with malignant genital lesions has not been studied because of the lack of an in vitro system and the unavailability of virions. We have now used virus-like particles (VLPs) of HPV type 33 to analyze the initial events in the interaction of the HPV capsid with cell lines. Binding of VLPs to HeLa cells was observed in biochemical assays and by immunofluorescence. VLP binding was inhibited by antisera raised against VLPs but not by monoclonal antibodies recognizing either L1 or L2 epitopes accessible on VLPs. Under saturating conditions, approximately 2 x 10(4) VLPs were bound per cell, with a dissociation constant of about 100 pM…
Assembly and Translocation of Papillomavirus Capsid Proteins
2002
ABSTRACT The major and minor capsid proteins of polyomavirus are preassembled in the cytoplasm and translocated to the nucleus only as a VP1-VP2/VP3 complex. In this study, we describe independent nuclear translocation of the L1 major protein and the L2 minor capsid protein of human papillomavirus type 33 by several approaches. First, we observed that expression and nuclear translocation of L2 in natural lesions precede expression of L1. Second, using a cell culture system for coexpression, we found that accumulation of L2 in nuclear domain 10 (ND10) subnuclear structures precedes L1 by several hours. In contrast, complexes of L2 and mutants of L1 forced to assemble in the cytoplasm are tra…
Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations.
2001
ABSTRACT Studies of the Hepatitis C virus (HCV) replication cycle have been made possible with the development of subgenomic selectable RNAs that replicate autonomously in cultured cells. In these replicons the region encoding the HCV structural proteins was replaced by the neomycin phosphotransferase gene, allowing the selection of transfected cells that support high-level replication of these RNAs. Subsequent analyses revealed that, within selected cells, HCV RNAs had acquired adaptive mutations that increased the efficiency of colony formation by an unknown mechanism. Using a panel of replicons that differed in their degrees of cell culture adaptation, in this study we show that adaptive…
Viral and cellular determinants of hepatitis C virus RNA replication in cell culture.
2003
Studies on the replication of hepatitis C virus (HCV) have been facilitated by the development of selectable subgenomic replicons replicating in the human hepatoma cell line Huh-7 at a surprisingly high level. Analysis of the replicon population in selected cells revealed the occurrence of cell culture-adaptive mutations that enhance RNA replication substantially. To gain a better understanding of HCV cell culture adaptation, we characterized conserved mutations identified by sequence analysis of 26 independent replicon cell clones for their effect on RNA replication. Mutations enhancing replication were found in nearly every nonstructural (NS) protein, and they could be subdivided into at …