Search results for "SPD"

In Vitro Bioactivity of Astaxanthin and Peptides from Hydrolisates of Shrimp (Parapenaeus longirostris) By-Products: From the Extraction Process to B…

2021

Non-edible parts of crustaceans could be a rich source of valuable bioactive compounds such as the carotenoid astaxanthin and peptides, which have well-recognized beneficial effects. These compounds are widely used in nutraceuticals and pharmaceuticals, and their market is rapidly growing, suggesting the need to find alternative sources. The aim of this work was to set up a pilot-scale protocol for the reutilization of by-products of processed shrimp, in order to address the utilization of this valuable biomass for nutraceutical and pharmaceuticals application, through the extraction of astaxanthin-enriched oil and antioxidant-rich protein hydrolysates. Astaxanthin (AST) was obtained using …

Effects Of Variation Of The Color Characteristics Of Lighting Sources By The Environmental Context

2012

Over recent years the importance of using daylight in indoor environments to achieve reductions of energy consumption was largely discussed, but the use of daylight is even more important because of its effects on human beings. Daylight has a more uniform spectral distribution than the artificial sources (especially discharge lamps) and consequently it allows a better color perception; moreover it changes during the day both in intensity and spectral power distribution (SPD). For all these reasons, daylight ensures a high comfort in carrying out all the human activities and is also important to the circadian rhythm regulation; therefore it results necessary to evaluate the entrance of natur…

Hsp60 Inhibitors and Modulators

2019

In this chapter, we focus on the 60 KDa Heat Shock Protein (Hsp60) and discuss some of its biological, molecular and pathological features. The structural and mechanistic aspect of the Hsp60 folding cycle will be also presented. We further illustrate how Hsp60 may be involved in many diseases and therefore considered as an effective therapeutic or theranostic target. Finally, the state-of-the-art on the development of Hsp60 and bacterial GroEL inhibitors and modulators of their expression will be illustrated. This is discussed in the light of a negative chaperonotherapy, and the consequent development of inhibitors, as well as positive chaperonotherapy, in the event its excessive activity i…

Occurrence of fourteen mycotoxins in tiger-nuts

2012

Abstract A previous developed matrix solid-phase dispersion (MSPD) extraction method was applied for the routine analysis of aflatoxins (AFs), ochratoxin A (OTA), fumonisins (FB 1 and FB 2 ), beauvericin (BEA), nivalenol (NIV), deoxynivalenol (DON), the toxin T-2 (T-2), toxin HT-2 (HT-2), diacetoxyscirpenol (DAS) and zearalenone (ZEN) in tiger-nuts by liquid chromatography–triple-quadrupole linear ion trap (HPLC–QTRAP ® ). The extraction solid support used was C 18 , while the elution solvent was acetonitrile/methanol (50/50, v/v) 1 mM ammonium formate. Using matrix-matched calibration, recoveries and repeatabilities were in the range 67–89% and 2–11% relative standard deviation (RSD), resp…

Applicability of hybrid linear ion trap-high resolution mass spectrometry and quadrupole-linear ion trap-mass spectrometry for mycotoxin analysis in …

2012

Abstract Recent developments in mass spectrometers have created a paradoxical situation; different mass spectrometers are available, each of them with their specific strengths and drawbacks. Hybrid instruments try to unify several advantages in one instrument. In this study two of wide-used hybrid instruments were compared: hybrid quadrupole-linear ion trap-mass spectrometry (QTRAP ® ) and the hybrid linear ion trap-high resolution mass spectrometry (LTQ-Orbitrap ® ). Both instruments were applied to detect the presence of 18 selected mycotoxins in baby food. Analytical parameters were validated according to 2002/657/CE. Limits of quantification (LOQs) obtained by QTRAP ® instrument ranged …

Application of an HPLC-MS/MS method for mycotoxin analysis in commercial baby foods

2012

Abstract This article describes the validation of an analytical method for the detection of 21 mycotoxins in baby food. The analytical method is based on the simultaneous extraction of selected mycotoxins by matrix solid-phase dispersion (MSPD) followed by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) using a hybrid triple quadrupole-linear ion trap mass spectrometer (QTRAP®). Information Dependent Acquisition (IDA), an extra confirmation tool for samples that contain the selected mycotoxins, was used. The matrix effects were evaluated, and the corrections for the matrix effects were performed using two calibration approaches: external matrix-matched calibration and…

Chaperonopathies and chaperonotherapy. Hsp60 as therapeutic target in cancer: potential benefits and risks.

2013

In this minireview we focus on Hsp60 as a target for anticancer therapy. We discuss the new concepts of chaperonopathies and chaperonotherapy and present information on Hsp60 localization in the cell membrane of human tumor cells. We describe novel mechanisms for Hsp60 reaching the extracellular environment that involve membrane-associated stages, as well as data on anti-Hsp60 antibodies found in human sera, both in normal subjects and patients affected by autoimmune diseases. Finally, we discuss possible therapeutic applications of anti-Hsp60 antibodies in cancer treatment, evaluating also side effects on non-tumor cells. In conclusion, the way for investigating Hsp60-targeted anti-tumor t…

La derecha europea y el nacional-integrismo

2002

Prediction and Surveillance Sampling Assessment in Plant Nurseries and Fields

2022

In this paper, we propose a structured additive regression (STAR) model for modeling the occurrence of a disease in fields or nurseries. The methodological approach involves a Gaussian field (GF) affected by a spatial process represented by an approximation to a Gaussian Markov random field (GMRF). This modeling allows the building of maps with prediction probabilities regarding the presence of a disease in plants using Bayesian kriging. The advantage of this modeling is its computational benefit when compared with known spatial hierarchical models and with the Bayesian inference based on Markov chain Monte Carlo (MCMC) methods. Inference through the use of the integrated nested Laplace app…

Indoor artificial lighting: Prediction of the circadian effects of different spectral power distributions.

2014

The study presented in this paper aims to evaluate if and how the environment affects the circadian impact of a light source by predicting circadian stimulus (CS) resulting from the exposure to different light scenes, using equations reported in studies by Rea et al. The main findings are: (1) Above 600 lx at eye level the increase of CS is little; (2) Between 50 lx and 200 lx at the eye level an increase of only 50 lx determines a significant increase of CS; (3) Light sources with correlated colour temperatures between 2900 K and 4200 K have similar impacts on the circadian system according to the calculation method used; (4) Even an almost neutral environment affects the circadian impact…