Search results for "SSH"

The pattern of histone H4 acetylation on the X chromosome during spermatogenesis of the desert locustSchistocerca gregaria

1996

We have used antibodies directed against histone H4 acetylated at lysine residue 5, 8, 12, or 16 and indirect immunofluorescence microscopy to probe chromosomes from spermatogonia and spermatocytes of the desert locust, Schistocerca gregaria. The autosomes showed bright overall fluorescence, indicative of high levels of H4 acetylation. In contrast, the X chromosome, which is facultatively heterochromatic during spermatogenesis of the locust, remained completely unstained in spermatogonia and secondary spermatocytes and showed only a small terminal fluorescent band in primary spermatocytes. This band probably corresponds to centromere-associated constitutive heterochromatin. Thus, underacety…

Molecular Cloning and mRNA Expression of Heat Shock Protein Genes and Their Response to Cadmium Stress in the Grasshopper Oxya chinensis

2015

Heat shock proteins (Hsps) are highly conserved molecular chaperones that are synthesized in response to stress. In this study, we cloned the full-length sequences of the Grp78 (glucose-regulated protein 78), Hsp70, Hsp90, and Hsp40 genes from the Chinese rice grasshopper Oxya chinensis. The full-length cDNA sequences of OcGrp78, OcHsp70, OcHsp90, and OcHsp40 contain open reading frames of 1947, 1920, 2172, and 1042 bp that encode proteins of 649, 640, 724, and 347 amino acids, respectively. Fluorescent real-time quantitative PCR (RT-qPCR) was performed to quantify the relative transcript levels of these Hsp genes in different tissues and developmental stages. The mRNAs encoding these four …

Regulation of fructose 2,6-bisphosphate in perfused flight muscle of the locust, Locusta migratoria: The effect of octopamine

1991

An optimisation model for daily manpower allocation in transhipment container terminals

2011

This work is motivated by the manpower allocation problem arising at the Cagliari International Terminal Container. Generally speaking, human resources allocation plays a key role in terminals with high labor costs, in order to achieve high levels of productivity and quality services for customers. As a result, terminal containers exhibit a growing interest in advanced decision support systems, in which optimization methods play a crucial role. In this talk we present an optimization model for manpower allocation in transshipment container terminals. The objective is to determine the optimal daily allocation of crane operators and trailer drivers at an operational planning level, taking int…

Effect of Fiber-Reinforced Composite and Elastic Post on the Fracture Resistance of Premolars with Root Canal Treatment—An In Vitro Pilot Study

2020

(1) Background: To analyze the fracture resistance of endodontically upper premolar teeth restored with glass fiber reinforced posts, glass fiber elastic posts, conventional composite resin (CR) and glass fiber reinforced composite (FRC) resins as restorations. (2) Methods: Seventy premolars were submitted to root canal treatment and restored with the following restorative materials (n = 10): A. FRC posts restored with resin

The impact of restorative material and ceramic thickness on CAD\CAM endocrowns

2019

Made available in DSpace on 2020-12-12T02:39:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Background: Endocrown restorations as a conservative approach to restore endodontically treated teeth still need an in vitro investigation under fatigue at different materials. This study evaluated the effect of restorative material and restoration thickness on the maximum fracture load of endocrowns subjected to cyclic loading. Material and Methods: Sixty (60) third molar teeth received an endocrown preparation with three different heights of remaining dental tissue (1.5, 3.0 or 4.5 mm). A leucite-based ceramic (LEU) and a lithium disilicate (LD) based ceramic were selected to manuf…

Forecasting container transshipment in Germany

2009

International audience; In this paper, we examine container transshipment at German ports using the seasonal ARIMA (SARIMA) model and the Holt-Winters exponential smoothing approach. Our models are designed especially to take account of the seasonal behavior of the quarterly data used. We consider the dynamic development in this sector for the whole container throughput and also the destinations Asia, Europe and North America, which are the world's three main economic regions. Our data runs from the first quarter of 1989 to the fourth quarter of 2006. We provide detailed quarterly forecasts for the year 2007 and 2008. According to forecasting error measures such as Mean Square Error and The…

Genes differentially expressed by Aspergillus carbonarius strains under ochratoxin A producing conditions

2010

Aspergillus carbonarius is an important ochratoxin A (OTA)-producing fungus that is responsible for toxin contamination of grapes and wine, coffee and cocoa. A suppression subtractive hybridization (SSH) approach was performed with two strains of A. carbonarius, antagonistic in their OTA-production ability, to identify genes whose expression is linked with the ability to produce OTA. BlastX analysis identified 109 differentially-expressed sequences putatively involved in the production of OTA, with significant similarities (Evalue < 10− 5) to sequences deposited in the NCBI non-redundant protein database. Of the 109 ESTs, 26% were involved in regulation processes, 15% corresponded to hypoth…

Shear bond strength of ceramic bracket bonded to different surface-treated ceramic materials

2018

Background This study evaluated the effect of ceramic surface treatments on bond strength of ceramic brackets to machine-able ceramics and ceramic veneering metal. Material and Methods Machined ceramic specimens (10x10x1.5 mm) were prepared from Empress® CAD (EP), and e.max® CAD (EM). Ceramic veneering metal specimens (PF) were fabricated from sintered d.Sign® porcelain (1.27 mm thickness) over d.Sign®10 metal (0.23 mm thickness). Each ceramic was divided into 3-groups and treated surface by Er-YAG laser (LE) or etching with 9.6% HF acid for 5 seconds (A5) or 15 seconds (A15). Resin adhesive (Transbond™-XT) was used for attaching ceramic brackets for each group (n=15) and cured with LED (Bl…

Shear bond strength of ceramic fused to CAD-CAM milled alloys

2018

Background This study evaluated the metal ceramic bond strength of cast Ni-Cr, cast Co-Cr, sintered Co-Cr and milled Co-Cr alloys to ceramic through two application procedures including the ceramic layering technique and ceramic pressed-on technique. Material and Methods Ceramic materials (Ø 8 mm, 1.5 mm thickness) were veneered by either the layering or pressed-on technique to cast Ni-Cr, cast Co-Cr, sintered Co-Cr and hard milled Co-Cr alloy disc (12 × 12 × 0.5 mm) (n=15). All specimens were treated with a thermal cycle process for 500 cycles at the temperature between 5 °C and 55 °C with immerse time of 30 seconds and 5 seconds for specimen transfer. The shear bond strength was determine…