Search results for "SSR"
showing 10 items of 378 documents
Identification and characterization of five polymorphic microsatellite loci in the freshwater copepod Hemidiaptomus gurneyi (Copepoda: Calanoida: Dia…
2016
Hemidiaptomus diaptomid copepods are known to be excellent biological indicators for the highly biodiverse crustacean communities inhabiting Mediterranean temporary ponds (MTPs), an endangered inland water habitat whose conservation is considered a priority according to the “Habitat Directive” of the European Union. This study reports on the characterization of five polymorphic microsatellite loci in Hemidiaptomus gurneyi, to be used as markers for fine-scale studies on the population genetic structure and metapopulation dynamics of a typical and obligate MTP dweller. The five selected loci proved to be polymorphic in the species, with three to five polymorphic loci per studied population. …
Transcriptome analysis and codominant markers development in caper, a drought tolerant orphan crop with medicinal value.
2019
AbstractCaper (Capparis spinosa L.) is a xerophytic shrub cultivated for its flower buds and fruits, used as food and for their medicinal properties. Breeding programs and even proper taxonomic classification of the genus Capparis has been hampered so far by the lack of reliable genetic information and molecular markers. Here, we present the first genomic resource for C. spinosa, generated by transcriptomic approach and de novo assembly. The sequencing effort produced nearly 80 million clean reads assembled into 124,723 unitranscripts. Careful annotation and comparison with public databases revealed homologs to genes with a key role in important metabolic pathways linked to abiotic stress t…
Characterisation of microsatellite and SNP markers from Miseq and genotyping-by-sequencing data among parapatric Urophora cardui (Tephritidae) popula…
2017
Phylogeographic analyses of the gall flyUrophora carduihave in earlier studies based on allozymes and mtDNA identified small-scale, parapatrically diverged populations within an expanding Western Palearctic population. However, the low polymorphism of these markers prohibited an accurate delimitation of the evolutionary origin of the parapatric divergence.Urophora carduifrom the Western Palearctic have been introduced into Canada as biological control agents of the host plantCirsium arvense. Here, we characterise 12 microsatellite loci with hexa-, penta- and tetra-nucleotide repeat motifs and report a genotyping-by-sequencing SNP protocol. We test the markers for genetic variation among thr…
Identification of novel compounds against three targets of SARS CoV-2 coronavirus by combined virtual screening and supervised machine learning.
2021
Coronavirus disease 2019 (COVID-19) is a major threat worldwide due to its fast spreading. As yet, there are no established drugs available. Speeding up drug discovery is urgently required. We applied a workflow of combined in silico methods (virtual drug screening, molecular docking and supervised machine learning algorithms) to identify novel drug candidates against COVID-19. We constructed chemical libraries consisting of FDA-approved drugs for drug repositioning and of natural compound datasets from literature mining and the ZINC database to select compounds interacting with SARS-CoV-2 target proteins (spike protein, nucleocapsid protein, and 2′-o-ribose methyltransferase). Supported by…
The interpreter as a citizen diplomat
2019
Abstract The article presents a case of interpretation as a political activity during the Cold War. In the 1980s and 1990s, a grassroots citizen diplomacy movement was initiated by the Californian Esalen Institute, the center of the American Human Potential Movement. In and around its Soviet-American exchange program, numerous individuals, NGOs and organizations established personal relationships and professional exchange with citizens of the two super powers and travelled in both directions. Interpreters had a complex and crucial role in this exchange which was different from both the professional experience of conference and of communal interpreting.
Transforming student contributions into subject-specific expression
2021
Drawing on a corpus of pre-service teacher training classroom interactions in an English-medium instruction university in Turkey, we examine teacher follow-up turns that introduce specialized terms, showing how a teacher transforms student’s responses into pedagogically relevant points using academic language. We argue that teacher third-turns following student contributions accomplish several interrelated actions, not only introducing new terminology to these teachers-in-training, but also familiarizing them with ways of thinking specific to their discipline, i.e., these turns model “doing being a teacher.” These teacher actions are used to bridge student contributions to more scientific t…
Exploring translanguaging in CLIL
2016
After reviewing the concepts of Content and Language Integrated Learning (CLIL) and Translanguaging, this article presents an exploratory study of translanguaging in CLIL contexts. Employing illustrative extracts from a collection of CLIL classroom recordings in Austria, Finland and Spain, we argue that both pedagogic and interpersonal motivations can influence language choices. We suggest that the L1 should be appreciated as a potentially valuable tool in bilingual learning situations and that there is a need for increased awareness-raising around this question. peerReviewed
Flipped classroom in COVID-19 times: a cross-talking perspective
2020
La COVID-19 ha generado una serie de cambios en las diferentes esferas de nuestras vidas. Estos cambios han sido especialmente visibles en el ámbito educativo, donde ha sido necesario repensar los procesos formativos de forma urgente en términos de no presencialidad. En base a esta situación, el presente trabajo presenta una experiencia de Flipped learning llevada a cabo con los estudiantes del primer curso del Grado en Pedagogía de la Universitat de València. Asimismo, se propone un análisis sobre la concepción previa de los estudiantes sobre esta metodología y su percepción tras finalizar su implementación, ofreciendo una visión trasversal. Para ello, se ha recogido la información de los …
Transforming a class from the Psychology degree into a <em>flipped classroom</em>
2016
La flipped classroom es una metodologia docente que trata de modificar la dinamica de la clase tradicional, en la que el alumnado estudia la materia antes de asistir a clase (fundamentalmente mediante Tecnologias de la Informacion y Comunicacion), y el tiempo en el aula se emplea para clarificar contenidos y realizar actividades significativas, con el apoyo continuado del profesorado. Estudios recientes muestran que se han obtenido resultados positivos respecto a la utilizacion de esta metodologia. Sin embargo, la implantacion de esta metodologia en el grado en Psicologia es escasa. Por ello, el objetivo de este trabajo fue aplicar la metodologia flipped classroom en un tema de la asignatur…
‘What’s the Moment Thingy?’– On the Emergence of Subject-Specific Knowledge in CLIL Classroom Interaction
2017
Situated in the European CLIL context where mainstream schools may opt for teaching content subjects through the medium of a foreign or second language, this paper explores secondary school physics classrooms, taught through English in Finland. The focus is on the role of classroom interaction in the emergence of subject-specific knowledge during six consecutive lessons, with particular attention to how one key concept in physics, ‘moment’, is handled. This micro-longitudinal approach shows that while the students are struggling between the everyday and the academic meanings of the word ‘moment’ throughout, there are also clear signs of progression. These signs show, for example, in student…