Search results for "STAI"

“BEP” RNAs and Proteins Are Situated in the Animal Side of Sea Urchin Unfertilized Egg, Which Can Be Recognized by Female Pronuclear Localization

1996

Microsurgery experiments demonstrate that the animal side of the unfertilized sea urchin Paracentrotus lividus egg coincides with the side of the egg pronucleus location. It is demonstrated by means of in situ hybridization and immunostaining of whole mounts of animal or vegetal halves that the previously identified bep 1 and bep4 RNAs and their proteins are located in the animal part of the unfertilized egg and much less in the vegetal part. The addition of Fabs against BEP1 and BEP4 causes exogastrulation.

Specific binding  of Bacillus thuringiensis Cry2A insecticidal proteins to a common site in the midgut of Helicoverpa species

2008

ABSTRACT For a long time, it has been assumed that the mode of action of Cry2A toxins was unique and different from that of other three-domain Cry toxins due to their apparent nonspecific and unsaturable binding to an unlimited number of receptors. However, based on the homology of the tertiary structure among three-domain Cry toxins, similar modes of action for all of them are expected. To confirm this hypothesis, binding assays were carried out with 125 I-labeled Cry2Ab. Saturation assays showed that Cry2Ab binds in a specific and saturable manner to brush border membrane vesicles (BBMVs) of Helicoverpa armigera . Homologous-competition assays with 125 I-Cry2Ab demonstrated that this toxi…

Bioresource technology for bioenergy, bioproductsenvironmental sustainability.

2022

The 3rd International Conference for Bioresource Technology for Bioenergy, Bioproducts & Environmental Sustainability (BIORESTEC) was held online from 17 to 19 May 2021. More than 120 participants from all over the world participated in this conference. The conference was studded with nine plenary lectures, ten invited lectures as well as more than 50 oral presentations along with several poster presentations. The purpose of the conference was to foster the exchange of knowledge and ideas among researchers in academia and industry as well as policymakers working on various aspects of biorefinery to discuss the progress made during the intervening three years and the latest trends in bio…

BIOFORTIFICATION AND INNOVATIVE SUSTAINABLE AGRONOMIC PRACTICES TO ENHANCE YIELD AND QUALITY OF LEAFY AND FRUITING VEGETABLES

2022

Retrograde transport of sodium selenite and intracellular injection of micro-ruby: a combined method to describe the morphology of zinc-rich neurones.

2003

Abstract Zinc is found in synaptic vesicles in a large number of glutamatergic systems. Its involvement in neurotransmission and neurological disorders has been suggested. There are methods for tracing these circuits, but they do not fill the dendritic tree. In this study, extracellular selenite injections in vivo were combined with intracellular injection of fluorochromes in fixed tissue to reveal the morphology of these zinc-rich neurones. Intraperitoneal and intracerebral injections of sodium selenite alone or intracerebral injections of selenite combined with bisbenzimide were made in the visual cortex of the rat in order to locate the somata of zinc-rich neurones. After 24 h of retrogr…

A comparative life-cycle assessment of asphalt mixtures for railway sub-ballast containing alternative materials

2018

Abstract Bituminous sub-ballast in railway track-bed can mitigate the variation of the moisture content in the subgrade and reduce vertical stiffness variations of the track leading to a more durable infrastructure. Nevertheless, durability is only one of the aspects that affects the sustainability of an infrastructure. Other relevant aspects are related to the environmental and economic issues. This research work joins the worldwide effort towards a paradigm shift in civil engineering devoted to assess the sustainability of infrastructures at the design stage. With this in mind, in this study different alternative bituminous sub-ballast mixtures containing recycled materials, namely crumb …

A novel flash glucose monitoring system in patients with diabetes mellitus type 1 and 2: which are the pieces of evidence for payer decision-makers?

2018

A rigorous health technology assessment is necessary to evaluate a new technology. However, healthcare regulatory agencies have less restrictive rules about medical devices, despite some recent warnings about this relevant matter. The evaluation should have at least two key issues, which require attention. The first one is its effectiveness, and the second one is its economic sustainability. In this paper, we deal with a novel glucose-sensing technology as a case study to examine the matter in depth. An evidence-based point of view is used to highlight this important issue.

Amyloid precursor protein in platelets: A peripheral marker for the diagnosis of sporadic AD

2001

BACKGROUND: An altered pattern of amyloid precursor protein (APP) forms consisting in a reduced ratio between the upper (130 kDa) and the lower (106 to 110 kDa) immunoreactivity bands has been described in platelets of patients with AD. OBJECTIVE: To evaluate the sensitivity and the specificity of platelet APP forms' ratio (APPr) as a marker for AD. METHODS: Eighty-five patients with probable AD and 95 control subjects (CON), including healthy individuals and neurologic patients, entered the study. Platelet APPr was evaluated by means of Western Blot analysis and immunostaining in the whole platelet homogenate, and calculated by the ratio between the optical density (OD) of the upper (130 k…

Enhancement of immunohistochemical staining

1982

The present paper describes two simple procedures which enhance immunohistochemical staining. One is to cover the sections with a plastic film to keep the serum uniformly distributed and minimize its evaporation. Secondly, rocking of the slides has been introduced, causing the serum to flow back and forth under the plastic film. Using this system, it has been possible to test for the effect of mixing on an immunohistochemical reaction (the demonstration of calcitonin in thyroid C cells). It has been found that mixing definitely enhances the reaction during the first 8 h. No effect of serum volume was observed.

Ecotourism in Bolivia : an ethnographical study of ecotourism impacts in Madidi National Park

2016

Master thesis development management - University of Agder 2016 The concepts of ecotourism are widely defined but it is often used as a marketing instrument in order to promote tourism businesses related to nature. Ecotourism experience a fast growth in world tourism industry and many developing countries are trying to use ecotourism as a tool to achieve sustainable development. Ecotourism provides many beneficiaries to local environments, economies and socio-cultural lives. However, ecotourism may as well have harmful effects on those involved. Still, ecotourism conserves and protects local areas and its people. Madidi National Park in Bolivia has been estimated to be one of the world’s mo…