Search results for "STRAIN"

Site-specific DBCO modification of DEC205 antibody for polymer conjugation

2018

The design of multifunctional polymer-based vectors, forming pDNA vaccines, offers great potential in cancer immune therapy. The transfection of dendritic immune cells (DCs) with tumour antigen-encoding pDNA leads to an activation of the immune system to combat tumour cells. In this work, we investigated the chemical attachment of DEC205 antibodies (aDEC205) as DC-targeting structures to polyplexes of P(Lys)-b-P(HPMA). The conjugation of a synthetic block copolymer and a biomacromolecule with various functionalities (aDEC205) requires bioorthogonal techniques to avoid side reactions. Click chemistry and in particular the strain-promoted alkyne-azide cycloaddition (SPAAC) can provide the req…

Formulations and exact algorithms for the distance-constrained generalized directed rural postman problem

2017

[EN] The generalized directed rural postman problem is an arc routing problem with many interesting real-life applications, such as routing for meter reading. In this application, a vehicle with a receiver travels through a series of neighborhoods. If the vehicle gets closer than a certain distance to a meter, the receiver is able to record the gas, water, or electricity consumption. Therefore, the vehicle does not need to traverse every street, but only a few, to get close enough to each meter. We study an extension of this problem in which a fleet of vehicles is available. Given the characteristics of the mentioned application, the vehicles have no capacities but there is a maximum distan…

Stress-strain analysis of specimens subjected to tensile loading during moisture uptake

2015

Abstract: A robust and efficient numerical method for the calculation of the internal stress state that develops within structures subjected to mechanical and steady state or transient hygroscopic loading conditions, has been developed. The method encompasses a layer by layer approach whereby the structure is discretised into plies with different material properties corresponding to the different ply moisture content. The proposed method has been validated against finite element solutions, and results from its application on a fully characterised EDT (technical name) polymer binder are presented. The impact of the moisture induced viscoelastic behaviour on the structural response of the cas…

Search for a Dark Leptophilic Scalar in e(+) e(-) Collisions

2020

Many scenarios of physics beyond the standard model predict the existence of new gauge singlets, which might be substantially lighter than the weak scale. The experimental constraints on additional scalars with masses in the MeV to GeV range could be significantly weakened if they interact predominantly with leptons rather than quarks. At an e+e- collider, such a leptophilic scalar (φL) would be produced predominantly through radiation from a τ lepton. We report herein a search for e+e-→τ+τ-φL, φL→ℓ+ℓ- (ℓ=e, μ) using data collected by the BABAR experiment at SLAC. No significant signal is observed, and we set limits on the φL coupling to leptons in the range 0.04<mφL<7.0 GeV. These bounds s…

Tensió laboral, síndrome d’esgotament professional (burn-out) i característiques sociodemogràfiques i laborals en treballadors de botigues de conveni…

2019

espanolLas tendencias de mercado actuales y las nuevas necesidades de los consumidores han situado a las tiendas de conveniencia como un formato comercial solido en nuestro pais. Las caracteristicas de estos establecimientos llevan a sus trabajadores a experimentar condiciones laborales diferentes, que pueden desembocar en la experimentacion por parte de los empleados de consecuencias negativas, tanto fisicas como psicosociales. El proposito del presente estudio ha sido analizar las posibles relaciones existentes entre el sindrome de bur-nout y la tension laboral con diversos factores sociodemograficos –sexo, edad, estado civil y nivel educativo– y laborales –tipo de contrato, numero de hor…

Acid sensitivity of neomycin-resistant mutants ofOenococcus oeni: a relationship between reduction of ATPase activity and lack of malolactic activity

1999

Mutants of Oenococcus oeni were isolated as spontaneous neomycin-resistant mutants. Three of these mutants harbored a significantly reduced ATPase activity that represented 50% of that of the wild-type strain. Their growth rates were also impaired at pH 5.3 (46-86% of the wild-type level). However, the profiles of sugar consumption appeared identical to those of the parental strain. At pH 3.2, all the mutant strains failed to grow and a drastic decrease in viability was observed after an acid shock. Surprisingly, all the isolated mutants were devoid of malolactic activity. These results suggest that the ATPase and malolactic activities of O. oeni are linked to each other and play a crucial …

Partially competitive inhibition of intestinal baclofen absorption by beta-alanine, a nonessential dietary aminoacid.

1991

In situ intestinal absorption of baclofen in the rat in the presence of beta-alanine has been investigated. Through the perfusion of 0.50 mM baclofen solutions containing variable concentrations of the aminoacid (from 5 to 100 mM), a partially competitive inhibition of baclofen absorption was characterized: absorption rate pseudoconstants of the spasmolytic drug decrease as beta-alanine concentration increases, until a limiting value is obtained (36.8 per cent of that found for baclofen alone). A computer method was developed in order to calculate parameters governing baclofen absorption in the presence of beta-aminoacid, with the following results: Vm = 11.22 mM h-1; Km = 7.42 mM; Ki = 2.4…

Kinetics of the intestinal uptake of zinc acexamate in normal and zinc-depleted rats.

1990

Abstract The uptake of zinc as acexamic acid salt in the small intestine of the anaesthetized rat was shown to be a two-phase process in normal animals. The first phase is rapid mucosal binding which satisfies the Freundlich isotherm equation and which involves about 30 per cent of the initially perfused zinc. The second phase was characterized as an apparent absorption step which obeys Michaelis-Menten and first-order combined kinetics, with the following parameters: Vm = 6.51 mg h−1; Km = 2.96 mg; ka = 0.306 h−1. In largely non-saturated conditions, an apparent global rate constant of about 2.50 h−1 was calculated. No significant interference due to endogenous zinc excretion into the smal…

2015

This paper provides a commentary on the academic literature on accounting conservatism with a view to highlighting the insights of that literature that are potentially useful for accounting standar...

Effects of electrical stimulation of the superior cervical ganglia on the number of ?synaptic? ribbons and the activity of melatonin-forming enzymes …

1989

Melatonin metabolism in the mammalian pineal gland is under the clear influence of sympathetic fibers originating in the superior cervical ganglia (SCG). Previous studies suggested that pineal "synaptic" ribbons (SR) as well are regulated by the gland's sympathetic innervation. To gain more insight into the mechanisms involved, we examined the effects of sympathetic stimulation on SR number and on the activity of melatonin forming enzymes, serotonin N-acetyltransferase (NAT) and hydroxyindole-O-methyltransferase (HIOMT). The SCG in adult male rats were stimulated electrically during daytime for either 15 or 120 min. Immediately following stimulation, the glands were removed and processed fo…