Search results for "Saccharomyces Cerevisiae"
showing 10 items of 738 documents
Raugu Saccharomyces cerevisiae žāvēšanas rezistences mehanismu pētījumi
2017
. Tika veikta divu rauga Saccharomyces cerevisiae celmu salīdzināšana - mēreni rezistenta S. cerevisiae 14 un izturīga pret žāvēšanu S. cerevisiae 77 celma, audzēšanai izmantojot dažādus barotņu sastāvus. Ir pētītas rauga šūnu to citoplazmatiskās membrānas funkcionālā stāvokļa izmaiņas pārejot anabiozes stāvoklī. Vēlāk par vispiemērotāko tika izvēlēta YPG barotne. Turpinot eksperimentus rauga biomasa pirms dehidratācijas tika 3 stundas inkubēta dažādos sķīdumos ar augstu osmotisko spiedienu, t.i. laktozes un ksilīta šķīdumi. Tas deva iespēju apskatīt šūnu izdzīvotības palielināšanas spējas un novērtēt izmaiņas šūnu lipīdu uzkrāšanās pēc dehidratācijas. Darbā tika izmantotas vairākas …
The influence of yeast glycosylated proteins on tannins aggregation in model solution
2004
<p style="text-align: justify;">The incidence of glycosylated yeast proteins on tannins aggregation in model solution was investigated using the spectrophotometric method (absorbance 700 nm). Glycosylated proteins released by two commercial <em>Saccharomyces cerevisiae</em> strains (RC212 and BM 45) during alcoholic fermentation in synthetic media, glycosylated proteins extracted by Peat’s method and industrial glycosylated proteins purified and separated by chromatography on Sepharose Concanavalin A were used to visualize effects on tannins aggregation. Results showed that tannins aggregation was limited by the glycosylated proteins according to their origin and their mod…
Growth Pattern of Saccharomyces cerevisiae in Cassava Mill Effluents
2018
Nigeria is the world leading producer of cassava. During processing of gari from cassava tuber large volume of effluents are discharged in the environment which is toxic to the environment and some of its associated biota. This study evaluated the growth pattern of Saccharomyces cerevisiae in cassava mill effluents. The Saccharomyces cerevisiae was isolated from palm wine following standard microbiological procedure. The Saccharomyces cerevisiae was inoculated into the sterile effluents and incubated for 15 days. At every 3days interval, 1ml of the effluents was obtained from the medium and the population density determined. Results of the growth showed that the population of Saccharomyces …
Changes in the Pi uptake and polyP accumulation in Saccharomyces cerevisiae strains deficient in the synthesis of trehalose and/or glycerol
2007
Abstract The intracellular level of free inorganic orthophosphate (P i ) in yeast cells generally depends on the P i uptake capacity, energy state of the cells in respect to the activity of the membrane-associated ATPases and on the activity of metabolic pathways involved in the production of glycerol and trehalose. Batch fermentation was performed to investigate the carbon substrate consumption, the P i uptake capacity and product formation by four Saccharomyces cerevisiae strains differing in their ability to produce glycerol and/or trehalose. The consumption of P i in mutant strains with a lack of the synthesis of the trehalose and/or glycerol exceeded the level for a wild type strain ab…
Chromatin structure of the 5′ flanking region of the yeastLEU2 gene
1989
The chromatin structure of theLEU2 gene and its flanks has been studied by means of nuclease digestion, both with micrococcal nuclease and DNase I. The gene is organized in an array of positioned nucleosomes. Within the promoter region, the nucleosome positioning places the regulatory sequences, putative TATA box and upstream activator sequence outside the nucleosomal cores. The tRNA3 Leu gene possesses a characteristic structure and is protected against nucleases. Most of the 5′ flank is sensitive to DNase I digestion, although no clear hypersensitive sites were found. The chromatin structure is independent of either the transcriptional state of the gene or the chromosomal or episomal loca…
In vivo assembly of chromatin on pBR322 sequences cloned into yeast plasmids
1989
Abstract In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there is any preference for nucleosome assembly along pBR322 sequences, we have used an indirect end labeling method. The results indicate that most nucleosomes are placed at random on pBR322, although the probability for histone octamers to interact with some short regions is somewhat reduced. These regions coincide with sequences in which the frequency distribution of nucl…
Immobilization of <i>Saccharomyces cerevisiae</i> Cells to Protein G-Sepharose by Cell Wall Engineering
2003
In this work, we explored the possibility of using the targeting of a heterologous protein to the cell wall of <i>Saccharomyces cerevisiae</i>, by fusing it to a cell wall protein, to construct yeast strains whose cells display on their surface proteins that bind to a matrix, so as to achieve the immobilization of the whole cells. With this aim, we created a gene fusion that comprises the region responsible for attachment of a cell wall protein to the cell wall, and the IgG binding region of staphylococcal protein A, and expressed it in the <i>mnn1mnn9</i> strain of <i>S. cerevisiae</i>. The surface display of the protein A-Icwp fusion protein was positiv…
Anhydrobiosis in yeast: FT-IR spectroscopic studies of yeast grown under conditions of severe oxygen limitation
2014
Anhydrobiosis is a unique state of living organisms when metabolism is temporarily and reversibly delayed in response to the extreme desiccation of cells. The production of dry active preparations of yeast grown under anaerobic conditions is not currently possible because preparations are extremely sensitive to the dehydration procedure, though they could be very helpful in different biotechnological processes, including bioethanol production. To characterize mechanisms responsible for such sensitivity to the dehydration procedure, Fourier transform infrared spectroscopy was used to study the composition of aerobically grown yeast Saccharomyces cerevisiae resistant to dehydration and grown …
A singleFKShomologue inYarrowia lipolyticais essential for viability
2002
The synthesis of β-1,3-glucan, the structural component of the yeast cell wall which gives shape to the cell, occurs at the plasma membrane and is the result of the activity of at least a two-component complex. Fks1p is the catalytic subunit directly responsible for the synthesis of β-1,3-glucan, whilst the second subunit, Rho1p, has a GTP-dependent regulatory role. FKS1 has been characterized in Saccharomyces cerevisiae, where its function is at least partially redundant with that of FKS2/GSC2. FKS homologues have also been identified in several other fungal species, including Candida albicans, Schizosaccharomyces pombe, Aspergillus nidulans, Cryptococcus neoformans and Paracoccidiodes bra…
Acetaldehyd als Indicator f�r die Regulation von Atmung und G�rung bei der aeroben Verg�rung von Glucose durch Saccharomyces cerevisiae
1971
Wahrend der aeroben Vergarung von Glucose wurde die Konzentration von Acetaldehyd im Garmedium uber den gesamten Garablauf bei mehreren Stammen von Saccharomyces cerevisiae verfolgt. Die Aldehydkonzentration weist bei Glucosekonzentrationen zwischen 5 und 20% zwei Maxima auf. Damit ist der Konzentrationsverlauf von Acetaldehyd aerob wesentlich anders als bei der anaeroben Garung, mit nur einem meist niedrigen Maximum. 10-3 M Azid hemmt die Bildung von Acetaldehyd ganz oder weitgehend. Das deutet auf die Funktion bzw. Synthese der Cytochrome, die in Gegenwart von Sauerstoff offensichtlich auch bei hohen Glucosekonzentrationen nicht vollstandig reprimiert werden. Der durch die Atmung bedingte…