Search results for "Silos"

Lack of Functional Trehalase Activity in Candida parapsilosis Increases Susceptibility to Itraconazole

2022

Central metabolic pathways may play a major role in the virulence of pathogenic fungi. Here, we have investigated the susceptibility of a Candida parapsilosis mutant deficient in trehalase activity (atc1Δ/ntc1Δ strain) to the azolic compounds Fluconazole and Itraconazole. A time-course exposure to Itraconazole but not Fluconazole induced a significant degree of cell-killing in mutant cells compared to the parental strain. Flow cytometry determinations indicated that Itraconazole was able to induce a marked production of endogenous ROS together with a simultaneous increase in membrane potential, these effects being irrelevant after Fluconazole addition. Furthermore, only …

Characterization of the Differential Pathogenicity of Candida auris in a Galleria mellonella Infection Model

2021

ABSTRACT Candida auris is an emergent multidrug-resistant fungal pathogen considered a severe global threat due to its capacity to cause nosocomial outbreaks and deep-seated infections with high transmissibility and mortality. However, evidence on its pathogenicity and the complex host-pathogen interactions is still limited. This study used the in vivo invertebrate model in Galleria mellonella to assess its virulence, exploring the mortality kinetics, melanization response, and morphological changes after fungal infection compared to Candida albicans and Candida parapsilosis, with known high and low pathogenicity, respectively. All C. auris isolates presented less virulence than C. albicans…

Molecular characterization of Italian Candida parapsilosis isolates reveals the cryptic presence of the newly described species Candida orthopsilosis…

2012

The authors report the molecular characterization of Candida parapsilosis isolates recovered from the blood and venous central catheter tips of patients admitted to different care units of the Polyclinic Hospital, University of Messina, Italy. Among 97 presumed C. parapsilosis isolates examined, 94 were identified as C. parapsilosis sensu stricto and the remaining 3 isolates were found to belong to the cryptic species Candida orthopsilosis which was recovered only from blood cultures of neonates (<30 days old) born prematurely. No C. metapsilosis was found in this study. This study emphasizes the role of C. parapsilosis as an important nosocomial pathogen, and it also describes, for the fir…

Microsatellite-based genotyping of Candida parapsilosis sensu stricto isolates reveals dominance and persistence of a particular epidemiological clon…

2012

In this study, using multilocus microsatellite analysis, we report the genetic characterization of 27 Candida parapsilosis isolates recovered in two different periods of time (2007-2009 and 2011-2012) from infants hospitalized in the neonatal intensive care unit of a hospital in Messina, Italy. The results revealed the persistence and dominance of a particular infectious genotype among NICU patients and highlight the power of the used microsatellite markers in clarifying epidemiologic associations, detect micro-evolutionary variations and facilitating the recognition of outbreaks. © 2012 Elsevier B.V.

CANDIDA SEPTIC THROMBOSIS OF THE LEFT ATRIUM IN TWINS: REPORT OF TWO CASES

2009

Background: Preterms are often exposed to nosocomial infections in NICU. Candida infections are particularly common and can result in progressive organization of intracardiac thrombosis, usually in the right atrium. Design and population: GB (24.4 wg, 460 g, bigeminal pregnancy): he was affected by RDS, PDA, jaundice, anaemia and had been submitted to TPN with CVC, also because of anus imperforate. On the 50th day, haemoculture resulted positive for Candida parapsilosis and, by echocardiography, hyperecogen peduncolate formation in the appendix of left auricola. Despite antifungal therapy, exitus occurred. CM (32.4 wg, 1390 g, bigeminal pregnancy): on 2nd day, she was operated for “apple-pe…

La qualità tecnologica utile di prodotti edilizi innovativi

2014

Study of green Sicilian table olive fermentations through microbiological, chemical and sensory analyses.

2010

The production of five different green table olive cultivars was studied by a combined strategy consisting of chemical, microbiological and sensory analyses. Cultivable microflora of samples collected during processing was monitored by plate counts on seven synthetic culture media. In all samples Enterobacteriaceae, Pseudomonaceae, staphylococci, lactic acid bacteria and spore-forming bacteria were undetectable. Yeasts and moulds were countable from the day 42 (2 log CFU/ml) till the end of fermentation (6 log CFU/ml). The use of three different approaches for microorganism detection, including a culture-independent methodology, revealed the presence of barely three yeast species during the…

Silage of Prickly Pears (Opuntia spp.) Juice By-Products

2020

Simple Summary The cactus pear plant is a Cactaceae which originates from the Americas and is highly resistant to arid and hot environments. The plant is used as animal feedstuff in regions characterized by a lack of nutrient resources. In Italy, the fruits are known as prickly pears are usually eaten fresh by humans, and more recently have also started to be transformed into fruit juice. Various by-products (i.e., peel, pulp, and seeds) derived from the extraction of fruit juice are used for livestock feed due to their high amounts of fermentable structural carbohydrates. However, some of these by-products are difficult to conserve due to their high fermentability. The aim of the present s…

Nuevas aportaciones al horizonte del bronce final de La Vital (Gandia, València)

2013

Durante las tareas de seguimiento arqueológico de las obras de construcción del Acceso Sur a Gandia se localizaron diferentes estructuras negativas datadas en el Bronce final ubicadas en el perímetro de protección del yacimiento de La Vital. Las estructuras documentadas corresponden a un lugar de hábitat fechado en estos momentos. En este trabajo presentamos los resultados de dicha actuación, valorándolos en el marco del desarrollo histórico de este periodo en el propio yacimiento y en la costa mediterránea peninsular.

Settlement, radiocarbon dates and craft productions during Late Neolithic and Bell Beaker periods in the ‘corredor de Montesa’ (Valencia, Spain). The…

2014

[EN] In this paper we present the results related to the excavation works conducted in Late Neolithic and Bell Beaker sites of Quintaret (Montesa, Valencia) and Corcot (l’Alcúdia de Crespins, Valencia), both in the middle valley of Canyoles river. The sites, neighboring each other, offer a quite different volume of information (Quintaret: 51 structures; Corcot: 4 structures). 14C dates allow us to define the occupation of this area of the valley along the first half of the III millennium cal BC. The presence of bell beaker ceramic in archaeological record of Quintaret is limited to just one structure (Q138), where at least 7 different vessels have been identified. Other prominent point of t…