Search results for "SoMe"
showing 10 items of 5114 documents
Surfing transcriptomic landscapes. A step beyond the annotation of chromosome 16 proteome
2013
All participating laboratories are members of ProteoRed-ISCIII.-- et al.
Mitochondrial proteomics profile points oxidative phosphorylation as main target for beauvericin and enniatin B mixture
2020
Abstract Beauvericin (BEA) and enniatin B (EN B) are non-legislated Fusarium mycotoxins usually found in cereal and cereal-based products all around the world. By the proteomic analysis of mitochondria enriched extracts from Jurkat cells exposed for 24 h to three concentrations of BEA:EN B (0.01–0.1–0.5 μM), a number of 1821 proteins (202 mitochondrial) were identified and relatively quantified. 340 proteins (59 mitochondrial) were statistically significant altered in our samples (Anova p-value ≤ 0.05 and fold change (FC) ≥1.5). The protein mitochondrial translational release factor 1 like (MTRF1L) was the most abundant protein in the three mycotoxin exposures studied. The mycotoxins mixtur…
BAG3 Proteomic Signature under Proteostasis Stress
2020
The multifunctional HSP70 co-chaperone BAG3 (BCL-2-associated athanogene 3) represents a key player in the quality control of the cellular proteostasis network. In response to stress, BAG3 specifically targets aggregation-prone proteins to the perinuclear aggresome and promotes their degradation via BAG3-mediated selective macroautophagy. To adapt cellular homeostasis to stress, BAG3 modulates and functions in various cellular processes and signaling pathways. Noteworthy, dysfunction and deregulation of BAG3 and its pathway are pathophysiologically linked to myopathies, cancer, and neurodegenerative disorders. Here, we report a BAG3 proteomic signature under proteostasis stress. To elucidat…
Surface analysis of Dicrocoelium dendriticum. The molecular characterization of exosomes reveals the presence of miRNAs
2013
Abstract With the aim of characterizing the molecules involved in the interaction of Dicrocoelium dendriticum adults and the host, we have performed proteomic analyses of the external surface of the parasite using the currently available datasets including the transcriptome of the related species Echinostoma caproni. We have identified 182 parasite proteins on the outermost surface of D. dendriticum. The presence of exosome-like vesicles in the ESP of D. dendriticum and their components has also been characterized. Using proteomic approaches, we have characterized 84 proteins in these vesicles. Interestingly, we have detected miRNA in D. dendriticum exosomes, thus representing the first rep…
Theileria parasites secrete a prolyl isomerase to maintain host leukocyte transformation
2015
Infectious agents develop intricate mechanisms to interact with host cell pathways and hijack their genetic and epigenetic machinery to change host cell phenotypic states. Among the Apicomplexa phylum of obligate intracellular parasites, which cause veterinary and human diseases, Theileria is the only genus that transforms its mammalian host cells. Theileria infection of bovine leukocytes induces proliferative and invasive phenotypes associated with activated signalling pathways, notably JNK and AP-1 (ref. 2). The transformed phenotypes are reversed by treatment with the theilericidal drug buparvaquone. We used comparative genomics to identify a homologue of the peptidyl-prolyl isomerase PI…
Current density maps, magnetizability, and nuclear magnetic shielding tensors of bis-heteropentalenes. II. Furo-furan Isomers
2004
Magnetic susceptibility and nuclear magnetic shielding at the nuclei of bis-heteropentalenes formed by two furan units ([2,3-b], [3,2-b], [3,4-b], and [3,4-c] isomers) have been computed by several approximated techniques and a large Gaussian basis set to achieve near Hartree–Fock estimates. Ab initio models of the ring currents induced by a magnetic field normal to the molecular plane were obtained for the three isomeric systems of higher symmetry, showing that the π electrons give rise to intense diamagnetic circulation. The π currents are responsible for enhanced magnetic anisotropy and strong out-of-plane proton deshielding. The theoretical findings are used to build up a “diatropicity …
Solution and thermal behaviour of novel dicationic imidazolium ionic liquids
2013
A new class of functionalised dicationic ionic liquids, containing a central cationic unit capped by a basic functionality (imidazole), has been synthesised. These salts have been characterised in isotropic solution using proton and 2D-NMR spectroscopy, and their thermal stability has been studied by DSC and TGA. All these novel salts contain the 1-(1-imidazolylmethyl)-3,5-di{1-(3'-octylimidazolylmethyl)}-benzene cation as a defining structural motif. Salts of both singly and doubly charged anions were prepared and, in particular, the selected monoanions (Br(-), [BF4](-), or [NTf2](-)) differ in size, shape and hydrogen-bonding ability, whereas the dianions differ in the nature of the space…
Molecular orbital studies on the mechanism of catalytic isomerization of xylenes III. Protonation channels
1981
Abstract The isoenergetic lines of the interaction energy between a bare proton and ortho-, meta- , and para -xylene have been calculated in order to detect both the best approaching channel and the preferred protonation positions. The channels found direct the protonation to the following ring positions: 4 and 5 for o -xylene, 4 and 6 for m -xylene, and 2 equivalents for p -xylene. The overall interaction energy determines the protonation positions on each isomer and the most important contributions to it are the electrostatic term in o -xylene, the polarization term in p -xylene, and both terms equally in m -xylene. These results are in agreement with the experimental findings and appear …
Chemically induced chromosome damage in early-developing embryos ofAnilocra physodes L. (Crustacea, Isopoda) following exposure to bis[Dimethyltin(IV…
1993
In order to obtain chromosome preparations from early-developing embryos of Anilocra physodes, a squash technique has been successfully employed. Results gathered after exposure of this material to bis[dimethyltin(IV)chloro]protoporphyrin IX {[(CH3)2SnCl]2 - Protoporphyrin IX} solutions at different exposure times suggest that this chemical complex is capable of producing abnormal metaphase and anaphase figures in proportion to its concentration and not to exposure length. Essentially, all of the chromosome abnormalities are classifiable as chromosome fragments mainly observed at the metaphase stage; chromosome bridges; and large decondensed chromosome regions.
ChemInform Abstract: Chemically Induced Chromosome Damage in Early-Developing Embryos of Anilocra Physodes L. (Crustacea, Isopoda) Following Exposure…
2010
In order to obtain chromosome preparations from early-developing embryos of Anilocra physodes, a squash technique has been successfully employed. Results gathered after exposure of this material to bis[dimethyltin(IV)chloro]protoporphyrin IX {[(CH3)2SnCl]2 - Protoporphyrin IX} solutions at different exposure times suggest that this chemical complex is capable of producing abnormal metaphase and anaphase figures in proportion to its concentration and not to exposure length. Essentially, all of the chromosome abnormalities are classifiable as chromosome fragments mainly observed at the metaphase stage; chromosome bridges; and large decondensed chromosome regions.