Search results for "Specificity."
showing 10 items of 2232 documents
New Insight into Immunity and Immunopathology of Rickettsial Diseases
2011
Human rickettsial diseases comprise a variety of clinical entities caused by microorganisms belonging to the generaRickettsia,Orientia,Ehrlichia, andAnaplasma. These microorganisms are characterized by a strictly intracellular location which has, for long, impaired their detailed study. In this paper, the critical steps taken by these microorganisms to play their pathogenic roles are discussed in detail on the basis of recent advances in our understanding of molecularRickettsia-host interactions, preferential target cells, virulence mechanisms, three-dimensional structures of bacteria effector proteins, upstream signalling pathways and signal transduction systems, and modulation of gene exp…
A reappraisal of the purported gastric pellet with Pterosaurian bones from the Upper Triassic of Italy
2015
A small accumulation of bones from the Norian (Upper Triassic) of the Seazza Brook Valley (Carnic Prealps, Northern Italy) was originally (1989) identified as a gastric pellet made of pterosaur skeletal elements. The specimen has been reported in literature as one of the very few cases of gastric ejecta containing pterosaur bones since then. However, the detailed analysis of the bones preserved in the pellet, their study by X-ray microCT, and the comparison with those of basal pterosaurs do not support a referral to the Pterosauria. Comparison with the osteology of a large sample of Middle-Late Triassic reptiles shows some affinity with the protorosaurians, mainly with Langobardisaurus pand…
Expression and characterization of the recombinant juvenile hormone epoxide hydrolase (JHEH) from Manduca sexta.
1998
The cDNA of the microsomal Juvenile Hormone Epoxide Hydrolase (JHEH) from Manduca sexta was expressed in vitro in the baculovirus system. In insect cell culture, the recombinant enzyme (Ms-JHEH) was produced at a high level (100 fold over background EH catalytic activity). As expected, Ms-JHEH was localized in the microsomal fraction with a molecular mass of approximately 50 kDa. Ms-JHEH showed a substrate and inhibitor spectrum similar to the wild type JHEH isolated from eggs of M. sexta. Its enzymatic activity was the highest for Juvenile Hormone III. Ms-JHEH hydrolyzed several trans-epoxides faster than cis-epoxides. A putative hydroxyl-acyl enzyme intermediate was isolated suggesting a …
De l’avoir lieu à l’être-lieu : parcours dans l’œuvre de Dorothy Cross
2019
Les installations et performances de Dorothy Cross, artiste contemporaine irlandaise, reposent sur un profond respect de la nature et visent à remettre en cause, de manière poétique, l’anthropocentrisme des sociétés occidentales. En mettant en scène le surgissement inattendu d’une bestialité archaïque symbolisée par le requin, la méduse ou les serpents, animaux sauvages mais culturellement marqués, et en s’immergeant dans des milieux et biotopes sauvages, l’artiste opère des renversements spatiaux : l’espace culturel devient un espace ensauvagé. Les procédés d’inscription, d’incrustation ou de projection de motifs évoquant une nature non domestiquée opèrent une symbiose entre l’homme et l’é…
Intraguild interactions between egg parasitoids: window of opportunity and fitness costs for a facultative hyperparasitoid.
2013
We investigated intraguild interactions between two egg parasitoids of Nezara viridula (L.) (Heteroptera: Pentatomidae), Ooencyrtus telenomicida (Vassiliev) (Hymenoptera: Encyrtidae) and Trissolcus basalis (Wollaston) (Hymenoptera: Platygastridae), as the former has the potential to be a facultative hyperparasitoid of the latter. We assessed the suitability of N. viridula eggs for the development of O. telenomicida as a function of egg age when they were unparasitized, or had been attacked by T. basalis at different times prior to exposure to O. telenomicida females. Ooencyrtus telenomicida can exploit healthy N. viridula host eggs up to 5 days of age, just prior to the emergence of N. viri…
Intraguild interactions between two egg parasitoids of a true bug in semi-field and field conditions.
2014
International audience; Research on interspecific competitive interactions among insect parasitoids has often been characterized by laboratory studies in which host insects are exposed to female parasitoids of different species in various sequences and combinations. In the last years, an increasing number of studies have investigated interspecific interactions under field and semi-field conditions although just a few number of works focused on egg parasitoids. In this work, we undertook a two-year study to investigate interspecific interactions between Trissolcus basalis (Wollaston) (Hymenoptera: Platygastridae) and Ooencyrtus telenomicida (Vassiliev) (Hymenoptera: Encyrtidae), two egg para…
Teachers’ Ability to Identify Children at Early Risk for Reading Difficulties in Grade 1
2017
The aim of the study was to investigate what kinds of assessment practices classroom teachers and special educational needs (SEN) teachers use in assessing first grade students' pre-reading skills (letter knowledge and phonological skills). Further, we investigated to what extent teachers were able to identify difficulties in pre-reading skills of the children with the lowest achievement scores. The accuracy of teacher ratings of students' pre-reading skills was studied by comparing teacher ratings to actual test scores. The data from two Finnish longitudinal studies were used: JLD sample (class teachers, n = 91; SEN teachers, n = 51; 200 students) and First Steps sample (class teachers, n …
Special Education Teachers’ Identification of Students’ Reading Difficulties in Grade 6
2020
his study investigated special educational needs (SEN) teachers’ (n = 29) assessment practices and the accuracy of their ratings of the students’ (M age = 12.75 years, n = 55) skill levels in reading fluency and reading comprehension. Teachers rated their sixth grade students’ fluency and comprehension on a three-point scale, and the students were also tested in group tests. Results showed that SEN teachers used several assessment practices simultaneously but mostly relied on observations. The correlations between the teacher ratings and the test scores were significant but moderate in fluency and weak in comprehension. Only two thirds of low-performing students having difficulties in fluen…
Monoclonal antibody 3H8: A useful tool in the diagnosis of candidiasis
1999
In a previous series of experiments six mAbs were obtained against cell wall extracts of Candida albicans ATCC 26555. After several studies only one of them, designated 3H8, has been used to produce a commercial kit for the rapid diagnosis of candidiasis, Bichro-latex albicans (Fomouze Diagnostics). The present study involved the generation and characterization of this mAb as an immunoglobulin G1 which recognizes mannoproteins of high molecular mass present in the C. albicans cell wall. ELISA assays showed that the presence of the epitope recognized by mAb 3H8 was similar in both yeast and mycelial cell walls of C. albicans, in contrast to the epitope for mAb 1B12, which is mainly expressed…
Monoclonal antibody TeM 106 reacts with a tonoplast intrinsic protein of 106 kDa from Brassica oleracea L
1995
A monoclonal antibody, designated TeM 106, that recognizes an intrinsic protein from the vacuole membrane (tonoplast) of cauliflower (Brassica oleracea L. var. botrytis) is described. Mice were immunized with a tonoplast fraction that had been purified from differentiating meristematic cells from the cauliflower head. Hybridomas were generated and screened by means of Enzyme Linked Immuno Sorbent Assays for differential reactivity to tonoplast over non-related proteins (bovine serum albumin). One out of 14 reactive murine clones was selected on the basis of its stability, secretory efficiency, and high affinity of the secreted antibodies. TeM 106 is an IgM which was shown by indirect immuno…