Search results for "Stalk"
showing 10 items of 116 documents
Maternal-fetal cross-talk: elucidating the role of mir-30d in endometrial receptivity and pregnancy outcome
2018
Introducción En el proceso de implantación embrionaria se produce una relación sincrónica entre el endometrio y el embrión que resulta fundamental para su correcta consecución; dicha sincronía se mantiene durante la gestación gracias a la existencia de una comunicación bidireccional entre la madre y el feto basada en la secreción de señales específicas que permiten regular un desarrollo cooperativo. Efectivamente, se ha observado como el embrión libera moléculas específicas del estado gestacional (ej.: gonadotropina coriónica humana o interferón tau en rumiantes), para prevenir la luteólisis inducida por la prostaglandina F2alpha, posibilitando la secreción continua de progesterona durante …
3 ′-5 ′ crosstalk contributes to transcriptional bursting
2019
Abstract Background Transcription in mammalian cells is a complex stochastic process involving shuttling of polymerase between genes and phase-separated liquid condensates. It occurs in bursts, which results in vastly different numbers of an mRNA species in isogenic cell populations. Several factors contributing to transcriptional bursting have been identified, usually classified as intrinsic, in other words local to single genes, or extrinsic, relating to the macroscopic state of the cell. However, some possible contributors have not been explored yet. Here, we focus on processes at the 3 ′ and 5 ′ ends of a gene that enable reinitiation of transcription upon termination. Results Using Bay…
Xrn1 influence on gene transcription results from the combination of general effects on elongating RNA pol II and gene-specific chromatin configurati…
2020
mRNA homoeostasis is favoured by crosstalk between transcription and degradation machineries. Both the Ccr4-Not and the Xrn1-decaysome complexes have been described to influence transcription. While Ccr4-Not has been shown to directly stimulate transcription elongation, the information available on how Xrn1 influences transcription is scarce and contradictory. In this study we have addressed this issue by mapping RNA polymerase II (RNA pol II) at high resolution, using CRAC and BioGRO-seq techniques in Saccharomyces cerevisiae. We found significant effects of Xrn1 perturbation on RNA pol II profiles across the genome. RNA pol II profiles at 5ʹ exhibited significant alterations that were com…
Extracellular vesicles in the human maternal-embryo crosstalk during the preconception period
2020
ANTECEDENTES: nuestro grupo demostró que el endometrio humano secreta exosomas (EXOs) al líquido endometrial (LE) con un contenido en miRNAs específico que puede ser internalizado por el embrión para favorecer la adhesión embrionaria. OBJETIVOS: definir y caracterizar los diferentes tipos de vesículas extracelulares (VEs) secretados por el endometrio humano al LE y estudiar su contenido en DNA, determinar si es internalizado por el embrión y si afecta a su regulación energética. MATERIALES Y MÉTODOS: El aislamiento de las VEs del LE se realizó mediante centrifugación diferencial. Se estudió su morfología, marcadores moleculares, tamaño y concentración a lo largo del ciclo menstrual. Su cont…
Ciò che dicono (e non dicono) le Sezioni Unite sul rapporto tra stalking, omicidio aggravato e lesioni aggravate
2022
Le Sezioni Unite hanno chiarito che il reato di omicidio commesso nei confronti della stessa persona offesa del reato di atti persecutori integra un reato complesso. Il presente contributo esamina le conseguenze che questo principio ha sul tema parallelo del rapporto tra il delitto di atti persecutori e il delitto di lesioni personali aggravate perché commesso dall'autore dello stalking nei confronti della stessa vittima. The United Sections have clarified that the crime of homicide committed against the same offended person as the crime of persecutory acts integrates a complex crime. This article examines the consequences that this principle has on the parallel theme of the relationship be…
Potential Roles of Muscle-Derived Extracellular Vesicles in Remodeling Cellular Microenvironment: Proposed Implications of the Exercise-Induced Myoki…
2021
Extracellular vesicles (EVs) have emerged as key players of intercellular communication and mediate crosstalk between tissues. Metastatic tumors release tumorigenic EVs, capable of pre-conditioning distal sites for organotropic metastasis. Growing evidence identifies muscle cell-derived EVs and myokines as potent mediators of cellular differentiation, proliferation, and metabolism. Muscle-derived EVs cargo myokines and other biological modulators like microRNAs, cytokines, chemokines, and prostaglandins hence, are likely to modulate the remodeling of niches in vital sites, such as liver and adipose tissues. Despite the scarcity of evidence to support a direct relationship between muscle-EVs…
Narrowed Family Spaces: Interlocking Couples in Intimate Partner Violence
2012
Starting from a presentation of the phenomenon of intimate partner violence and of its various possible forms, this paper presents an overview of the most recent theoretical contributions on the topic, the dynamics of abuse in the couple and the main psychodynamic aspects that explain the possible manifestations and peculiarities of the effects of violence, both from the point of view of the abuser and the victim. Through the analysis of a life story, using two different measures, the authors focus their attention on the particular interlocking relationship of the couple that makes it difficult for the victim to pull himself or herself out of the perverse relationship and on the mechanisms …
Victims' experiences concerning the actions of the authorities and the coping skills used for surviving partner stalking
2017
Tutkimuksemme on osa suomalaista VARJO-hanketta, jonka tarkoitus on kehittää palveluita eron jälkeisen vainon uhrien tueksi sekä ehkäisevään työhön ja kuntoutukseen liittyviä työmuotoja. Tutkimuksemme aineisto koostui naispuolisten vainon uhrien internetpohjaisen vertaisryhmän aikana käydyistä keskusteluista, joita tarkastelimme sisällönanalyysin avulla. Tutkimuksemme tarkoitus oli lisätä tietoisuutta vainon uhrien kokemuksista liittyen viranomaisten toimintaan ja tutkintaprosessiin sekä selviytymiskeinoista, jotka he kokivat hyödyllisiksi yrittäessään selviytyä eron jälkeisestä vainosta. Löysimme neljä pääluokkaa kuvaamaan uhrien kokemuksia viranomaistoiminnasta ja tutkintaprosessista. Näm…
Osservatorio della Corte costituzionale (n. 8/2014)
2014
Corte cost. n. 172 del 2014 (in tema di delitto di stalking e sufficiente determinatezza della fattispecie)
Gripas vīrusa hemaglutinīna fragmenta strukturāla izpēte un eksponēšana uz vīrusiem līdzīgo daļiņu virsmas
2015
Iekļaujot vakcīnā konservatīvus vīrusa proteīnu domēnus, būtu iespējams nodrošināt plaša spektra aizsardzību pret dažādiem gripas vīrusu apakštipiem. Viens no šādiem konservatīviem rajoniem ir hemaglutinīna (HA) stalka peptīds HA2.3. Bakalaura darba mērķis bija noteikt HA2.3 struktūru, tā eksponēšanas iespējas uz dažādu vīrusiem līdzīgo daļiņu (VLP) virsmas, un pētīt šo daļiņu imunoloģiskās īpašības. Pētījumā tika veikta HA2.3 ekspresija, attīrīšana, kristalizācija. Izmantojot rentgenstaru difrakciju, tika noteikts, ka HA2.3 struktūra vides pH 5,5 atbilst natīvajai HA stalka trimēra post fusion konformācijai. HA2.3 tika ķīmiski konjugēts pie dažādu VLP virsmas, kuras pēc tam izmantoja peļu …