Search results for "Subculture"
showing 10 items of 25 documents
Foreed flushing of branch segments as a method for obtaining reactive explants of mature Quercus robur trees for micropropagation
1994
The aim of this study was to micropropagate mature Quercus robur L. trees when material retaining physiologically juvenile characteristics (stump sprouts, epicormic shoots) is not available. Branch segments from 70–300 year-old trees were force-flushed and the flushed, partially rejuvenated or reinvigorated shoots were used as a source of explants for establishment of cultures. In vitro establishment and multiplication was achieved with seven of the eight selected trees. The proliferation capacity of cultures of vertically placed explants declined after several subcultures, but efficient shoot multiplication was achieved by culturing decapitated shoots placed horizontally on GD medium suppl…
Delinquent Boys: una critica
2020
Il saggio è basato su una relazione che presenta insieme a David Dietrick al Meeting annuale della Pacific Sociological Society nel 1958. I due autori, infatti, sostengono che la teoria della delinquenza elaborata da Cohen sia in grado di spiegare soltanto la formazione di una subcultura delinquente ma non il suo mantenimento che non può essere spiegato esclusivamente attraverso l’aspirazione verso i valori di classe media e la frustrazione derivante dal loro mancato conseguimento. Da una parte, infatti i due autori sostengono che il mantenimento di una subcultura deviante dipende da reazioni sociali negative ripetute e che aumentano di intensità, ragion per cui i giovani delinquenti si uni…
Elimination of in vitro bacterial contaminants in shoot cultures of ‘MRS 2/5’ plum hybrid by the use of Melia azedarach extracts
2008
The antimicrobial activity of leaf and callus extracts of Melia azedarach was tested on in vitro shoot cultures of the peach rootstoch 'MRS 2/5' (Prunus cerasifera × Prunus spinosa) that were heavily contaminated with Sphingomonas paucimobilis (Sp) and Bacillus circulans (Bc). The extracts were filter-sterilised and added at 0%, 1%, 5%, 10% and 20% to a modified Murashige and Skoog proliferation medium previously autoclave-sterilised. Up to about 17% shoots died with 10-20% extract, except for Sp-contaminated shoots, whose survival was reduced to 50% after treatment with 20% extract. No shoots died with 1% to 5% supplement. The undiluted leaf extract showed bactericidal activity on plated S…
Nörttikulttuurin identiteettikriisi
2020
Nörttikulttuuri on identiteettikriisissä. Perinteisesti nörttimäisinä pidetyt käytänteet ja kulttuurituotteet ovat siirtyneet lähemmäs valtavirtaa, mikä on häivyttänyt rajaa nörttien ja muiden median ja teknologian käyttäjien välillä. Nörttikulttuurin sisälle tämä on heijastunut kiistaksi siitä, kuka saa osallistua nörttikulttuurin kuluttamiseen, tuottamiseen ja määrittelyyn, ja millä tavoin. Stereotypia nörttikulttuurista valkoisten heteromiesten maskuliinisena kenttänä ei koskaan ole pitänyt täysin paikkansa, mutta 2000-luvulla väitteen totuudellisuus on murentunut entisestään.
 Tässä artikkelissa tarkastelemme nörttiyden kriisiä kolmen tapaustutkimuksen kautta ja analysoimme, mitä G…
Morphological and anatomical observations of abnormal somatic embryos from anther cultures of Citrus reticulata
2010
A morphological and anatomical study of regenerants obtained from mandarin anther culture was carried out. Beside morphologically normal somatic embryos, abnormal structures were originated in the course of somatic embryogenesis. Anatomical anomalies can be found at several growth stages, causing the formation of slender stems, stubby structures, non-functional leaves. When too long, some structures are subject to shedding, with the formation of various abscission zones. Most of them are subject to degeneration, although many are capable of further, localized, morphogenesis. A thorough knowledge of morphology and anatomy of normal and abnormal regenerants could make possible to select and s…
Micropropagation of adultLavandula dentataplants
1998
SummaryA protocol for in vitro propagation of adult Lavandula dentata plants has been achieved. Cultures were established by placing nodal segments on Murashige and Skoog medium containing BA, KIN, and NAA. Highest shoot multiplication rates were obtained when explants grown in the presence of 5.0 μM BA or 20 (JLM KIN were transferred to medium with 8.8 μM BA and 15% coconut milk. Multiplication efficiency through subcultures was significantly affected by the cytokinin concentration in the initial culture medium. Subculture reduced drastically the final number of shoots produced on nodal segments isolated from shoots grown in the presence of 2.0 μM BA or 40.0 μM KIN. Shoots were easily root…
Influence of culture conditions on embryo formation and maturation in auxin-induced embryogenic cultures of Digitalis obscura.
1994
Somatic embryogenesis was induced in hypocotyls of Digitalis obscura using indoleacetic acid or 2,4-dichlorophenoxyacetic acid with different culture and subculture conditions. Indoleacetic acid-induced embryogenic cultures were used to investigate the effects of amino acids, polyamines and growth regulators on embryo differentiation and maturation. Supplementation of the media with amino acids, polyamines or abscisic acid did not influence or had an adverse effect on embryogenic response. Gibberellic acid at 1.4 μM in either culture (30 days) or subculture medium was effective in promoting both differentiation and normal embryo development. The efficiency of somatic embryogenesis was great…
Morphogenic potential of mechanically isolated single cells from Digitalis obscura L. callus
1989
Calli from hypocotyl and root explants of Digitalis obscura L. showed regeneration of adventitious shoots, roots and embryos when transferred to Murashige & Skoog medium supplemented with cytokinins alone or in combination with auxins. Optimum shoot-bud formation was achieved in the presence of IAA and BA, while roots mainly appeared either in absence of growth regulators or with IAA and Kn. Embryo formation took place only in those combinations that included Kn. Embryo development was influenced by the type of auxin, and precocious germination occurred in media with NAA. Mechanically isolated cells from hypocotyl- and root-derived calli were plated in MS medium supplemented with several IA…
Organizational Culture Beyond Consensus and Clarity: Narratives From Elite Sport
2020
In sport psychology, organizational culture is usually depicted as shared, consistent, and clear—the glue that holds people together so they can achieve success. There is, however, growing discontent in sport psychology with this idea of culture and extensive critiques in other academic domains that suggest this perspective is limited. Accordingly, the authors draw on narrative interviews with participants (n = 7) from different areas of sport and use Martin and Meyerson’s three perspective (integration, differentiation, and fragmentation) approach to culture alongside thematic analysis to reconstruct three “ideal cases” that exemplify each perspective. The findings emphasize a different pa…