Search results for "TATA"

Distribution and Dynamics of Transcription-Associated Proteins during Parvovirus Infection

2012

ABSTRACT Canine parvovirus (CPV) infection leads to reorganization of nuclear proteinaceous subcompartments. Our studies showed that virus infection causes a time-dependent increase in the amount of viral nonstructural protein NS1 mRNA. Fluorescence recovery after photobleaching showed that the recovery kinetics of nuclear transcription-associated proteins, TATA binding protein (TBP), transcription factor IIB (TFIIB), and poly(A) binding protein nuclear 1 (PABPN1) were different in infected and noninfected cells, pointing to virus-induced alterations in binding dynamics of these proteins.

Reactive neurogenesis during regeneration of the lesioned medial cerebral cortex of lizards

1995

Abstract This study reports that lesion of the adult lizard medial cortex (lizard hipocampal fascia dentata) induces a short period of intensive neurogenesis which we have termed reactive neurogenesis; a cell proliferation event that occurs in the subjacent ependyma. Specific lesion of the medial cortex was achieved by intraperitoneal injection of the neurotoxin 3-acetylpyridine and proliferating cells were detected using tritiated thymidine or 5-bromodeoxiuridine pulse labelling. After lesion, granule neurons in the lizard medial cortex cell layer appeared pyknotic and died; they were then removed and progressively replaced by a set of new neurons. These neurons were mostly generated from …

Transitory disappearance of microglia during the regeneration of the lizard medial cortex

1994

In normal lizards, microglial cells populate the medial cortex (a zone homologous to the hippocampal fascia dentata), with a preferential distribution along the border between the granular cell layer and the plexiform layers. Intraperitoneal injection of the neurotoxin 3-acetylpyridine (3AP) induces a selective lesion in the medial cortex with a rapid degeneration of the granular layer and its zinc-enriched axonal projection. Within 6-8 weeks, the granular layer is, however, re- populated by a new set of neurons generated in the subjacent ependyma and the cell debris is removed. The aim of this study was to determine to what extent microglia were involved in the scavenging processes during …

Triterpene Saponins from the Roots of Achyranthes bidentata

2001

Three saponins, oleanolic acid-28- O -s-D-glucopyranoside (1), chikusetsusaponin V (2), and 3- O -s-D-glucopyranosyl-oleanolic acid-28- O -s-D-glucopyranoside (3) were isolated from the roots of Achyranthes bidentata Blume (Amaranthaceae). No activity was shown in the granulocyte phagocytosis test nor in the test of the potentiation of the cytotoxicity of cisplatin in human colon cancer cells. This is the first report of compounds 1, 2 and 3 isolated from Achyranthes species. Furthermore, the NMR data of 2 completed the partially published data.

Biosilicification of loricate choanoflagellate: organic composition of the nanotubular siliceous costal strips of Stephanoeca diplocostata.

2010

SUMMARY Loricate choanoflagellates (unicellular, eukaryotic flagellates; phylum Choanozoa) synthesize a basket-like siliceous lorica reinforced by costal strips (diameter of approximately 100 nm and length of 3 μm). In the present study, the composition of these siliceous costal strips is described, using Stephanoeca diplocostata as a model. Analyses by energy-dispersive X-ray spectroscopy (EDX), coupled with transmission electron microscopy (TEM), indicate that the costal strips comprise inorganic and organic components. The organic, proteinaceous scaffold contained one major polypeptide of mass 14 kDa that reacted with wheat germ agglutinin. Polyclonal antibodies were raised that allowed …

Extensive sampling sheds light on species-level diversity in Palearctic Placobdella (Annelida: Clitellata: Glossiphoniiformes)

2022

The bloodfeeding leech genus Placobdella is dominated by North American diversity, with only a single nominal species known from Central America and one from the Palearctic region. This is likely due to considerable underestimation of Palearctic biodiversity, but investigations into potential hidden diversity are lacking. To shed light on this, the present study introduces new data for specimens initially identified as Placobdella costata from Ukraine (close to the type locality), Italy, Germany, Latvia, Montenegro, Bulgaria, Slovenia, Turkey, Azerbaijan, Tunisia, and Algeria, and uses both nuclear (Internal Transcribed Spacer [ITS] region) and mitochondrial (cytochrome c oxidase subunit I …

Establishing the relative importance of sympatric definitive hosts in the transmission of the sealworm, Pseudoterranova decipiens: a host-community a…

2001

The importance of a given host to a particular parasite can be determined according to three different criteria: host preference, host physiological suitability and host contribution to transmission. Most studies on the sealworm Pseudoterranova decipiens have focussed on the latter factor, but few attempts have been made to develop a quantitative transmission model evaluating the relative importance of each host. The purpose of this study was to propose a flow-chart model to study sealworm transmission within a seal community. The model was applied to hypothetical data of four seal species acting as definitive hosts of P. decipiens sensu stricto in eastern Canada: harp seal Phoca groenlandi…

Poronia punctata, a rare ascomycetes from Italy

2007

Poronia punctata is reported from Sicily as a rare species. Ecological observations in comparison with literature data from Italy and other European countries demonstrated that the rarity of P. punctata should be attributed to environmental factors different from the presence or absence of suitable substrata.

L'hsp60 e l'hsp10 sono iperespresse durante la cancerogenesi prostatica

2003

RUOLO DEL COEFFICIENTE APPARENTE DI DIFFUSIONE (ADC) NELLO STUDIO DELLA PROSTATA IN RISONANZA MAGNETICA (RM)

2014

Materiali e Metodi: La nostra popolazione è costituita da 25 di età compresa tra 50 e 81 anni (media 63.95 +/- 9.54) sottoposti ad esame RM della prostata con apparecchiatura da 1.5 T mediante l'ausilio di bobina endorettale. Oltre al protocollo morfologico (T1w-T2w) , la valutazione spettroscopica e dinamica post-contrastografica, sono state acquisite sequenze DWI sul piano assiale con valori di b pari a 800/mm2. E' stato calcolato il valore di ADC in regioni di interesse poste in corrispondenza di aree di zona periferica (ZP) con aspetto normale ed aree che presentavano segnale ipointenso enne sequenze T2w e/o che mostravano restrizione della diffusione dell'acqua nelle sequenze DWI. All'…