Search results for "TLA"
showing 10 items of 1764 documents
C57BL/6-specific conditions for efficient in utero electroporation of the central nervous system.
2014
Abstract Background In utero electroporation is a fast an efficient tool to specifically address gene expression in the murine central nervous system. This technique was originally established in ICR/CD-1 outbred mice. Neuroanatomical differences between the different mouse strains and variations in gestation length require the optimization of the conditions for each strain to avoid severe complications. Furthermore the relevant position information is currently only scarcely standardized and not always easy to transfer to C57BL/6 mice. New method In this study we present an improved method for in utero electroporation of C57BL/6 including a detailed atlas that allows for specific and effic…
CD4(+) and CD8(+) anergic T cells induced by interleukin-10-treated human dendritic cells display antigen-specific suppressor activity.
2002
Interleukin-10 (IL-10)–treated dendritic cells (DCs) induce an alloantigen- or peptide-specific anergy in various CD4+ and CD8+ T-cell populations. In the present study, we analyzed whether these anergic T cells are able to regulate antigen-specific immunity. Coculture experiments revealed that alloantigen-specific anergic CD4+ and CD8+ T cells suppressed proliferation of syngeneic T cells in a dose-dependent manner. The same effect was observed when the hemagglutinin-specific CD4+T-cell clone HA1.7 or tyrosinase-specific CD8+ T cells were cocultured with anergic T cells of the same specificity. Anergic T cells did not induce an antigen-independent bystander inhibition. Suppression was depe…
Cellular and humoral immune responses against autoreactive T cells in multiple sclerosis patients after T cell vaccination.
1999
Myelin basic protein (MBP)-reactive T cells may play an important role in the autoimmune pathogenesis of multiple sclerosis (MS). MBP-reactive T cells can be specifically targeted by T cell vaccination, a procedure whereby MS patients are immunized with attenuated autologous MBP reactive T cells. T cell vaccination induces immune responses to the vaccine cells together with a depletion of MBP reactive T cells. Forty-nine MS patients were treated with T cell vaccination in an extended phase I trial to study the safety, immune responses and clinical effects of T cell vaccination. In the present paper the immune responses towards the vaccine cells were characterized. Substantial long-term in v…
Induction of CD4+/CD25+ regulatory T cells by targeting of antigens to immature dendritic cells
2003
AbstractCoupling of ovalbumin (OVA) to anti–DEC-205 monoclonal antibody (mAb) (αDEC) induced the proliferation of OVA-specific T cells in vivo. Expansion was short-lived, caused by dendritic cells (DCs), and rendered T cells anergic thereafter. Phenotypic analysis revealed the induction of CD25+/CTLA-4+ T cells suppressing proliferation and interleukin-2 (IL-2) production of effector CD4+ T cells. The findings were supported by 2 disease models: (1) CD4+ T-cell–mediated hypersensitivity reactions were suppressed by the injection of αDEC-OVA and (2) the application of hapten-coupled αDEC-205 reduced CD8+ T-cell–mediated allergic reactions. Thus, targeting of antigens to immature DCs through …
ICOS and CD28 reversely regulate IL-10 on re-activation of human effector T cells with mature dendritic cells
2002
With newly generated ICOS-ligand (ICOS-L)-specific monoclonal antibodies we determined that human Langerhans cells in situ express similar levels of ICOS-L, CD80, and CD86, compared to immature dendritic cells (DC) derived from monocytes in vitro. Maturation of DC strongly up-regulated CD80 and CD86 but did not significantly change ICOS-L levels. On coculture of "naive"CD4(+) T cells with mature DC in the presence of superantigen, ICOS was highly up-regulated on T cells, but played only a secondary role in the CD28-dominated release of TNF-alpha and IFN-gamma, and did not participate in the induction of IL-2. Cocultures of "effector" CD4(+) T cells with mature DC revealed CD28 as the drivin…
Alignment of the ATLAS Inner Detector and Single Top Studies
2016
Alineamiento del detector interno de ATLAS y estudios del single top Esta tesis esta compuesta por dos partes bien diferenciadas. La primera trata de los estudios de alineamiento del detector interno del experimento ATLAS en el CERN. La segunda se centra en los estudios sobre el quark top cuando es producido como partícula aislada. ATLAS es uno de los grandes experimentos del LHC (Gran colisionador de hadrones) situado en el CERN. ATLAS es un detector de propósito general. Se diseñó para aprovechar la alta energía disponible en el LHC y observar fenómenos de física a muy altas energías. ATLAS está formado por varios sub-sistemas, como el detector interno de trazas, los calorímetros electrom…
Update on the TowerJazz CMOS DMAPS development for the ATLAS ITk
2019
The upgrade of the ATLAS tracking detector for the High-Luminosity Large Hadron Collider at CERN requires the development of novel radiation hard silicon sensor technologies. For the de- velopment of depleted CMOS sensors for ATLAS we combined small electrodes with minimal capacitance and advanced processing for fully depleted active sensor volume to achieve radiation hard CMOS sensors in line with ATLAS ITk specifications. Based on initial studies on the prototype sensor “TowerJazz Investigator” we have now developed, produced and tested a first full-size depleted CMOS sensor based on the 180nm TowerJazz imag- ing process, the so-called “MALTA” sensor. The sensor combines special low-noise…
Dual compensation control-system for offshore logistic equipment
2012
In offshore applications and moreover in transports from ship-to-ship, the needs of highly sophisticated motion compensation solution are increasing. They consist of sensors, actuators and control units. To design the best mechatronic solution, it is necessary modeling the mechanical system in order to identify the transfer function and the dynamic behaviour of the system. Currently Active Heave Compensation system are widely used in order to compensate for heave vessel motion. between a vessel moving under sea state and a fixed base or viceversa. Several research project are ongoing in order to develop solution able to compensate undesired movements also in ship to ship offloading operatio…
Dataset and Data analysis for "Infrared based saliva screening test for COVID-19"
2021
External Modelling 1 is the Quasar .ows model to process the Source data 1 to generate the PCA plot shown in Figure 4B and loadings plots in Figure 4C. External Modelling 2 is the MCDCV-PLS-DA Matlab model that can be used in combination with PLS-Tool Box to generate the Receiver Operating Curves (ROC) and the prediction model shown in Figures 4D and 4E, respectively. Source Data 1 is for the PCA modelling. Source Data 2 for the MCDCV-PLS-DA.
Atlas Lingüístico de la Península Ibérica (ALPI): Estudi i edició del País Valencià
2001
La tesi doctoral “Atlas Lingüístico de la Península Ibérica (ALPI): Estudi i edició del País Valencià” pretén recuperar un material lingüístic (fonètic, morfosintàctic i lèxic) inèdit recollit al País Valencià entre 1934 (pobles castellanoparlants) i 1935 (pobles valencianoparlants) per investigadors de la talla de M. Sanchis Guarner i F. de B. Moll. La recuperació d’aquest material està feta de forma digital, escrita (edició de les enquestes) i il·lustrada per mapes lingüístics (cartografia selectiva). Es tracta d’unes enquestes lingüístiques projectades pel Centre d’Estudis Històrics de Madrid (precedent de l’actual CSIC), presidit per R. Menéndez Pidal, qui va encarregar el projecte al m…