Search results for "TRIACYLGLYCEROL"

showing 10 items of 29 documents

Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum.

2017

Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use in biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the sole energy source. Some diatoms, however, can also grow in a mixotrophic mode, wherein both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum , using glycerol as the source of reduced carbon. Transcriptomics, metabolomics, metabolic modelling and physiological data combine to indicate that glycerol affect…

0301 basic medicineGlycerol[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]LightMetabolic fluxBiologySettore BIO/19 - Microbiologia GeneralePhotosynthesisPhaeodactylum tricornutumGeneral Biochemistry Genetics and Molecular BiologyGlycerolipid03 medical and health sciencesNutrientmixotrophyBotanyMicroalgaeSettore BIO/04 - Fisiologia VegetaleMetabolomics[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biologyphotosynthèse14. Life underwaterPhaeodactylum tricornutumBiomassTranscriptomicsmétabolismemicro-algueDiatomsphotosynthesisPhototrophmarine diatomsfungiCarbon metabolismLipid metabolismArticlesapproche omiquebiology.organism_classificationCarbonTriacylglycerol biosynthesis030104 developmental biologyDiatomBiomass productionLipid metabolismBiochemistryGeneral Agricultural and Biological SciencesEnergy sourcemetabolismMixotrophomics analyses
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Association of liver steatosis with lipid oversecretion and hypotriglyceridaemia in C57BL/6j mice fed trans-10, cis-12-linoleic acid

2003

AbstractConjugated linoleic acids (CLA) have recently been recognized to reduce body fat and plasma lipids in some animals. This study demonstrated that the steatosis accompanying the fat loss induced by trans-10,cis-12-C18:2 (CLA2) and not cis-9,trans-11-C18:2 (CLA1) isomer in C57BL/6j mice was not due to an alteration of the liver lipoprotein production that was even increased. The 3-fold decrease in plasma triacylglycerol contents and the induction of mRNA expression of low-density lipoprotein receptors concomitantly observed in CLA2-fed mice suggested an increase in the lipoprotein clearance at the level of the liver itself. CLA1 feeding produced similar but attenuated effects on trigly…

030309 nutrition & dieteticsConjugated linoleic acidLiver steatosisLipoproteins VLDLBiochemistrychemistry.chemical_compoundMiceStructural BiologyLipoproteinReceptorComputingMilieux_MISCELLANEOUSchemistry.chemical_classification0303 health sciencesFatty AcidsLiverlipids (amino acids peptides and proteins)Conjugated linoleic acidmedicine.medical_specialtyLinoleic acidBiophysics[SDV.BC]Life Sciences [q-bio]/Cellular BiologyTriacylglycerolLinoleic Acid03 medical and health sciencesInternal medicineGeneticsmedicineAnimalsLow-density lipoprotein receptorRNA MessengerMolecular BiologyTriglycerides030304 developmental biologyDNA PrimersBase SequenceEsterificationMyocardiumBody WeightRNAFatty acidCell BiologyFatty acidmedicine.diseaseFatty LiverMice Inbred C57BLEndocrinologychemistryLDL receptorSteatosisLipoprotein
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Metabolic signatures across the full spectrum of non-alcoholic fatty liver disease.

2022

Funder: European Commission

ALTtype 2 diabetes mellitusROC receiving operator characteristicaspartate aminotransferaseHSDLDL low-density lipoproteinUHPLC ultrahigh-performance liquid chromatographyROCHCCNon-alcoholic steatohepatitisGCPCANASHGastroenterology2-HB 2-hydroxybutanoic acid; 3-HB 3-hydroxybutanoic acid; ALT alanine aminotransferase; AST aspartate aminotransferase; CE cholesterol ester; Cer ceramide; FFA free fatty acid; FLIP Fatty Liver Inhibition of Progression; Fibrosis; GC gas chromatography; HCC hepatocellular carcinoma; HSD honest significant difference; LC lipid cluster; LDL low-density lipoprotein; LM lipid and metabolite; LMC lipid metabolite and clinical variable; LPC lysophosphatidylcholine; Lipidomics; Mass spectrometry; Metabolomics; NAFL non-alcoholic fatty liver; NAFLD non-alcoholic fatty liver disease; NAS NASH activity score; NASH non-alcoholic steatohepatitis; NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network; NRR non-rejection rate; Non-alcoholic steatohepatitis; PC(O) ether PC; PC phosphatidylcholine; PCA principal component analysis; PE phosphatidylethanolamine; QTOFMS quadrupole-time-of-flight mass spectrometry; ROC receiving operator characteristic; SAF steatosis activity and fibrosis; SM sphingomyelin; T2DM type 2 diabetes mellitus; TG triacylglycerol; UHPLC ultrahigh-performance liquid chromatographySAFSAF steatosis activity and fibrosisLM lipid and metabolitehonest significant differenceHSD honest significant differenceTG triacylglycerolnon-rejection ratecholesterol esterPCPEGC gas chromatographyfree fatty acidFLIPNASH non-alcoholic steatohepatitisNIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkBIOMARKERST2DMPE phosphatidylethanolamineLDLlipidNAFLDFFA free fatty acid2-HBMetabolomicsNAFL non-alcoholic fatty liverLMCphosphatidylcholineScience & TechnologySM sphingomyelinGastroenterology & HepatologyMass spectrometryactivitynutritional and metabolic diseasesT2DM type 2 diabetes mellitusACIDSreceiving operator characteristicdigestive system diseasesquadrupole-time-of-flight mass spectrometryLC lipid clusterlow-density lipoproteinNAS2-HB 2-hydroxybutanoic acidNAS NASH activity scoreQTOFMSether PCNRRSCORING SYSTEMprincipal component analysisgas chromatographyLC2-hydroxybutanoic acidPROGRESSIONAST aspartate aminotransferaseLMPC phosphatidylcholinePC(O)MARKERSUHPLCsteatosisTOOLImmunology and AllergyINSULIN-RESISTANCECerSMFatty Liver Inhibition of Progressionhepatocellular carcinoma2-HB 2-hydroxybutanoic acid NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network NRR non-rejection rate Non-alcoholic steatohepatitis PC(O) ether PC PC phosphatidylcholine PCA principal component analysis PE phosphatidylethanolamine QTOFMS quadrupole-time-of-flight mass spectrometry ROC receiving operator characteristic SAF steatosis activity and fibrosis SM T2DM type 2 diabetes mellitus TG triacylglycerol UHPLC ultrahigh-performance liquid chromatographyultrahigh-performance liquid chromatographyCELPC3-HBNAFLnon-alcoholic fatty liverTGtriacylglycerolNRR non-rejection rateLife Sciences & BiomedicineNAFLD non-alcoholic fatty liver diseaseFLIP Fatty Liver Inhibition of Progressionalanine aminotransferasemetaboliteCer ceramideCE cholesterol estersphingomyelinlysophosphatidylcholineand fibrosisALT alanine aminotransferaseInternal MedicineceramideNational Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkAST3-HB 3-hydroxybutanoic acidQTOFMS quadrupole-time-of-flight mass spectrometryPCA principal component analysisLPC lysophosphatidylcholineHepatologynon-alcoholic fatty liver diseaseand clinical variablePC(O) ether PC3-hydroxybutanoic acidFibrosisNASH activity scoreNIDDK NASH-CRNlipid clusterlipid and metabolitephosphatidylethanolamineLipidomicsLMC lipid metabolite and clinical variableFFAHCC hepatocellular carcinomaJHEP reports : innovation in hepatology
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One-Pot Analysis: a New Integrated Methodology for Determination of TAG and FA Determination through LC/MS and in-silico Saponification

2017

Vegetable oils differ significantly for their lipid profile and are mainly constituted by triacylglycerols (TAGs). These are esters formed bonding glycerol to three fatty acids (FAs). To the best of our knowledge, FA and TAG profiles in oils and fats are obtained using at least two different experimental techniques. In particular, FA determination requires elaborated procedures for sample preparation and analyte derivatization. In this work, we propose a one-pot analysis able to determine both TAG and FA contents of edible oils, using experimental data obtained from a simple liquid chromatography/mass spectrometry determination of TAGs. This experimental procedure is followed by an in silic…

AnalyteMass spectrometryTriacylglycerol01 natural sciencesApplied Microbiology and BiotechnologyAnalytical Chemistrychemistry.chemical_compound0404 agricultural biotechnologyLiquid chromatography–mass spectrometryGlycerolSample preparationFood scienceSafety Risk Reliability and QualityDerivatizationChromatography010401 analytical chemistry04 agricultural and veterinary sciencesFatty acid040401 food scienceFAME0104 chemical sciencesLipid profileVegetable oilBlood chemistrychemistryIn silico saponificationSafety ResearchSaponificationFood ScienceFood Analytical Methods
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Direct HPLC Monitoring of Lipase Activity in Reverse Micellar Media

1995

Given the profusion of biotechnological applications of the nonaqueous use of lipases, we have evaluated the possibilities of exploiting the inherent advantages of high performance liquid chromatography (HPLC) for a simple, rapid assay of lipase activity in reverse micellar media, as a convenient alternative to previously reported spectroscopic methods, using both a model system and esterification reaction, and different commercial lipases. The results obtained after a screening for optimized chromatographic conditions in the reverse-phase mode indicate that a satisfactory resolution of the reaction components can be obtained following a straightforward protocol, which permits an accurate, …

Carboxylic Ester HydrolasesChromatographybiologyResolution (mass spectrometry)ChemistryRapid assaybiology.proteinTriacylglycerol lipaseMolecular MedicineModel systemEsterification reactionLipaseHigh-performance liquid chromatographyJournal of Liquid Chromatography
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PRELIMINARY CHEMICAL CHARACTERIZATION OF TUNISIN MONOVARIETAL VIRGIN OLIVE OILS AND COMPARISON WITH SICILIAN ONES.

2007

Work was carried out on the characterization of monovarietal virgin olive oils (VOO) from Tunisia and Sicily (Italy). The two main Tunisian VOO (cvv. Chétoui of the North and cv. Chemlali grown in the Center and some regions of the South) and three principal Sicilian VOO (cvv. Nocellara del Belice, Biancolilla and Cerasuola) were studied. Moreover, the Chétoui oils were tested in a rain-fed control and an irrigation regime. All olive samples were picked at three different stages of ripeness. Analyses of major components (fatty acids and triacylglycerols) and minor ones (squalene, tocopherols and phenolic compounds) were carried out. Chétoui oils had a higher level of phenolic compounds foll…

ChemistryCOMPOSITIONVIRGIN OLIVE OILGeneral ChemistryRipenessIndustrial and Manufacturing Engineeringlanguage.human_languageTRIACYLGLYCEROLSHorticultureVegetable oilANTIOXIDANTSBotanyEdible oillanguageSicilianPHENOLSFood ScienceBiotechnologyOlive oil
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Triacylglycerols in edible oils: Determination, characterization, quantitation, chemometric approach and evaluation of adulterations

2017

Vegetable oils are a dietary source of lipids that constitute an essential component of a healthy diet. The commonly used vegetable oils differ significantly for their triacylglycerol (TAG) profile. TAGs represent the principal components of oils and may contain different fatty acids (FA) esterified with glycerol leading to several positional isomers. To differentiate individual TAGs species in edible oils, advanced analysis systems and innovative methods are therefore required. TAGs can be considered as good fingerprints for quality control and many studies have been performed to develop rapid and low cost analytical methods to determinate the authenticity, origin and eventually evidence f…

Food AnalysiChemometricFood Contamination01 natural sciencesTriacylglycerolPlant OilTriglycerideBiochemistryQuantitationAnalytical ChemistryChemometricsEdibleDietary Fats UnsaturatedPlant OilsTriglyceridesChromatography010405 organic chemistryChemistryFatty Acids010401 analytical chemistryOrganic ChemistryGeneral MedicineHealthy dietOilFood Analysis0104 chemical sciencesAdulterationFood AnalysisFatty Acid
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Lipolytic activity of the yeast species associated with the fermentation/storage phase of ripe olive processing.

2010

9 páginas, 4 figuras, 5 tablas.-- El Pdf del artículo es la copia de autor.

Food HandlingSaccharomyces cerevisiaePopulationMolecular Sequence DataTriacylglycerol lipaseMicrobiologyFungal ProteinsOleaYeastsDNA Ribosomal SpacerFood scienceLipaseLipase activityeducationDNA FungalTable oliveseducation.field_of_studybiologyPichia membranifaciensfood and beveragesLipasebiology.organism_classificationYeastBiochemistryFruitFermentationbiology.proteinFermentationMolecular identificationBacteriaFood ScienceFood microbiology
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Pérdida aparente y cuantitativa de ácidos grasos y especies de triglicéridos a temperatura de fritura

2012

[EN]: Olive and sunflower oils were heated at 180 °C for 5, 10 and 15 hours with the aim of defining the changes in the contents of fatty acid methyl esters (FAME) and triacylglycerols (TAG) under frying conditions. Differences between apparent and real loss of FAME or TAG are defined for an adequate interpretation of the changes taking place at high temperature. Such differences depend on the expression of the results and frequently result in erroneous conclusions. Results showed that from the normalized composition it could be deduced that only the most unsaturated FAME (C18:2) or TAG containing it was significantly altered. However, quantitative data indicated that all the unsaturated FA…

FryingPolar compoundsFrituraTriacylglycerolsFatty acidsÉsteres metílicos de ácidos grasos polaresPolar FAMEÁcidos grasosCompuestos polaresTriglicéridos
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Lipase-catalysed preparation of acyl derivatives of the germacranolide cnicin

2009

Several acyl derivatives of cnicin were obtained through lipase-catalysed acylation and alcoholysis reactions. In most reactions lipases showed a regioselective behaviour affording only one product. Longer chain acyl derivatives were prepared at lower temperature than the used in lipase-catalysed reactions, to preclude side products formation. The enzymatic approach let to prepare a family of novel acetyl and fatty acid derivatives of cnicin which are not obtainable following traditional organic synthetic procedures. Fil: Monsalve, Leandro Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis…

GermacranolideTriacylglycerol lipaseBioengineeringBiochemistryCnicinCatalysisEnzyme catalysisAcylationchemistry.chemical_compoundOrganic chemistryLipasechemistry.chemical_classificationbiologyENZYME CATALYSISChemistryProcess Chemistry and TechnologyCiencias QuímicasRegioselectivityALCOHOLYSISCNICINACYLATIONSESQUITERPENOIDSEnzymeQuímica Orgánicabiology.proteinCIENCIAS NATURALES Y EXACTAS
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