Search results for "TRIF"

CCDC 1564495: Experimental Crystal Structure Determination

2017

Related Article: Fernando Rabasa-Alcañiz, Javier Torres, María Sánchez-Roselló, Tomás Tejero, Pedro Merino, Santos Fustero, Carlos del Pozo|2017|Adv.Synth.Catal.|359|3752|doi:10.1002/adsc.201700975

Chemical and Spectroscopical Properties of Medium Sizedtrans- andcis-Bicyclo[n.1.0]alk-2-ynes

1997

Chemical and spectroscopical properties of cis- and trans-cyclopropano-anellated cycloalkynes of medium ring size are investigated and compared with results from force field calculations. IR and 13C-NMR data of the alkynes reflect the deformation of the triple bond due to ring strain. Parallel to bending the reactivity increases with decreasing ring size, the trans fused alkynes prove to be nearly as reactive as the cis fused with a two carbon smaller ring size. The bicycloalkynes are complexed with silver triflate. 13C NMR reveals that the triple bond as well as the cyclopropane serve as ligands.

Synthesis of a New Disulfide Affinity Adsorbent for Purification of Human Uterine Progesterone Receptor

2005

For purification of the human uterine progesterone receptor, an affinity adsorbent was synthesized in which the specific ligand (16 alpha-ethyl-3-oxo-19nor-androst-4-ene 17 beta-carboxylic acid) was bound to derivatized celulose using a disulfide-group-containing spacer. The purified receptor protein, isolated by reductive cleavage of the disulfide bond, bound the synthetic gestagen R5020 with high affinity (Kd 12.2 nmol/l). The affinity gel was highly efficient. A 24000-fold purification of progesterone receptor with a recovery of 40% could be achieved in a single step within 6 h. By means of dodecyl sulphate/polyacrylamide gel electrophoresis two main polypeptides with molecular weights o…

Tecniche di biorisanamento delle acque di falda contaminate da nitrati

2014

Biological nitrogen removal from landfill leachate: a pilot-scale study

1999

A pilot-scale two-stage process including an anaerobic pretreatment (up-flow anaerobic sludge blanket, UASB) unit and a pre-denitrification activated sludge process was studied for treating leachates from a municipal landfill (waste aged 4 to 5 years, area 2.5 ha) and from a windrow composting area (1ha). A single-stage pilot process was used for nitrification studies. The leachate characteristics were as follows: COD 740 to 2400 mg 1-1 (chemical oxygen demand), BOD7 (biochemical oxygen demand) approximately 1000 mg 1 -1, Ntot 40 to 120 mg 1-1 and the temperature was between 2 and 21°C. The results show that 45 to 50% COD removal could be achieved in the UASB reactor even at temperatures b…

Determination of biogenic amines in meat by combined ion-exchange capillary gas chromatography

1984

Abstract A procedure is described for the determination of putrescine, cadaverine and histamine in meat. Crude perchloric acid extracts were pre-separated on a weakly acidic cation exchanger and the amines quantified by capillary gas chromatography. The diamines were analysed as trifluoroacetyl derivatives and histamine was converted into N α -trifluoroacetyl-N τ -ethoxycarbonylhistamine. The accuracy of the determination of diamines was examined by a precipitation pre-separation method and by mass fragmentometric quantification. The proposed procedure allows the sensitive, sufficiently precise and highly specific determination of putrescine and cadaverine in meat.

HONO Emissions from Soil Bacteria as a Major Source of Atmospheric Reactive Nitrogen

2013

From Soil to Sky Trace gases emitted either through the activity of microbial communities or from abiotic reactions in the soil influence atmospheric chemistry. In laboratory column experiments using several soil types, Oswald et al. (p. 1233 ) showed that soils from arid regions and farmlands can produce substantial quantities of nitric oxide (NO) and nitrous acid (HONO). Ammonia-oxidizing bacteria are the primary source of HONO at comparable levels to NO, thus serving as an important source of reactive nitrogen to the atmosphere.

Spatial distribution of N-cycling microbial communities showed complex patterns in constructed wetland sediments.

2013

International audience; Constructed wetlands are used for biological treatment of wastewater from agricultural lands carrying pollutants such as nitrates. Nitrogen removal in wetlands occurs from direct assimilation by plants and through microbial nitrification and denitrification. We investigated the spatial distribution of N-cycling microbial communities and genes involved in nitrification and denitrification in constructed wetland sediments receiving irrigation water. We used quantitative real-time PCR (qPCR) to characterize microbial communities. Geostatistical variance analysis was used to relate them with vegetation cover and biogeochemical sediment properties. The spatial distributio…

Batch Test Evaluation of Four Organic Substrates Suitable for Biological Groundwater Denitrification

2014

Nitrates pollution represents nowadays a serious issue related to the quality of groundwater; continuous growth of industrial-scale agricultures lead to an increase of nitrates content in groundwater in the last years. Several technologies have been validated as capable to promote in situ biological nitrates remediation, such as permeable reactive barriers (PRB), biotrench, biobarriers etc. These technologies are all characterised by the use of organic substrate that act as a slow release carbon source. In free dissolved oxygen absence, such organic carbon is further oxidised, by heterotrophic bacteria naturally present in soil, in compliance to anoxic metabolism by using nitrates bound oxy…

Implication of three isoforms of PLA2in human T-cell proliferation

2002

We observed that human (Jurkat) T-cells constitutively expressed the mRNA, encoding for the four isoforms of phospholipase A(2) (PLA(2)), i.e. two secretory (type IB and type V), and two cytosolic (type IV, Ca(2+)-dependent and type VI, Ca(2+)-independent). In order to assess whether these PLA(2) isoforms are active, we labeled Jurkat T-cells with [(3)H]arachidonic acid ([(3)H]AA) and determined its release into the extracellular medium in the presence of phorbol 12-myristate 13-acetate (PMA) and ionomycin. The three PLA(2) isoforms seem functional as aristolochic acid and bromoenol lactone (BEL), the respective inhibitors of type IB/type V and type VI PLA(2)s, significantly inhibited the r…