Search results for "TRIF"
showing 10 items of 1419 documents
In Vitro Release of Lectins From Phallusia mamillata Hemocytes After Their Fractionation on a Density Gradient
1993
Hemocytes were fractionated by centrifugation on a discontinuous Percoll density gradient from the hemolymph of Phallusia mamillata. Results obtained from microcultures of the fractionated hemocytes, sugar-inhibition experiments, SDS-PAGE, and immunoblotting indicate that “compartment cells” release cellular-type (CL) lectins that are specific for α-lactose and lactulose. The released lectins have the same properties as the CL lectins that were previously isolated from sonicated unfractionated hemocytes, but they differ in terms of some molecular and immunological properties from the lectins (SL) purified from the serum. SLs were never found in the supernatants from microcultures of the fra…
Determination of phenobarbital in plasma by micellar liquid chromatography
2000
A new liquid chromatographic procedure for the determination of phenobarbital in plasma samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C(18) analytical column, a guard column of similar characteristics, and a 0.03 M CTAB-3% 1-propanol at pH 7 mobile phase. The UV detector was set at 250 nm. Butabarbital was used as internal standard. Sample preparation only required the addition to the plasma samples of a 0.1 M SDS solution at pH 3 and centrifugation before injection into the chromatographic system. The limit of detection was 0.83 microg/mL of phenobarbital in plasma samples. The coefficients of variation were lower than 7. 5%.
Gastric histamine methyltransferase: Different methylation rates for enantiomers of side-chain methylated histamine analogues using a highly purified…
1984
The inhibitor/activator and substrate properties of enantiomers of two methylated histamines (MH) were investigated using a histamine methyltransferase preparation which was purified 1207-fold from pig fundic mucosa by ultracentrifugation, ion-exchange chromatography on DEAE-cellulose and preparative electrofocusing. In 1-100 microM concentrations, S-alpha-MH and R-alpha-MH were acceptor substrates as good as histamine itself. When substrate concentrations were increased to 1 mM these substances were methylated to an even greater extent than histamine, since they did not exert substrate inhibition on HMT. Introduction of a further methyl-group into the N alpha-position reduced acceptor subs…
Pectin biopolymer mechanics and microstructure associated with polysaccharide phase transitions.
2019
Polysaccharide polymers like pectin can demonstrate striking and reversible changes in their physical properties depending upon relatively small changes in water content. Recent interest in using pectin polysaccharides as mesothelial sealants suggests that water content, rather than nonphysiologic changes in temperature, may be a practical approach to optimize the physical properties of the pectin biopolymers. Here, we used humidified environments to manipulate the water content of dispersed solution of pectins with a high degree of methyl esterification (high-methoxyl pectin; HMP). The gel phase transition was identified by a nonlinear increase in compression resistance at a water content …
Selective Preparation of Diamondoid Phosphonates
2014
We present an effective sequence for the preparation of phosphonic acid derivatives of the diamondoids diamantane, triamantane, [121]tetramantane, and [1(2,3)4]pentamantane. The reactions of the corresponding diamondoid hydroxy derivatives with PCl3 in sulfuric or trifluoroacetic acid give mono- as well as didichlorophosphorylated diamondoids in high preparative yields.
Green phosphorescence and electroluminescence of sulfur pentafluoride-functionalized cationic iridium(III) complexes
2015
EZ-C acknowledges the University of St Andrews for financial support. We report four cationic iridium(III) complexes [Ir(C^N)2(dtBubpy)](PF6) that have sulfurpentafluoride-modified 1-phenylpyrazole and 2-phenylpyridine cyclometalating (C^N) ligands (dtBubpy = 4,4'-di-tert-butyl-2,2'-bipyridyl). Three of the complexes were characterized by single-crystal X-ray structure analysis. In cyclic voltammetry, the complexes undergo reversible oxidation of iridium(III) and irreversible reduction of the SF5 group. They emit bright green phosphorescence in acetonitrile solution and in thin films at room temperature, with emission maxima between 482–519 nm and photoluminescence quantum yields of up to 7…
Correlation between dynamical and structural heterogeneities in colloidal hard-sphere suspensions
2016
Experiments combining dynamic and static light scattering have probed a colloidal hard-sphere system for the formation of dynamical and structural heterogeneities, which play a role in both forms of solidification: crystallization and vitrification.
One pendulum to run them all
2013
The analytical solution for the three-dimensional linear pendulum in a rotating frame of reference is obtained, including Coriolis and centrifugal accelerations, and expressed in terms of initial conditions. This result offers the possibility of treating Foucault and Bravais pendula as trajectories of the same system of equations, each of them with particular initial conditions. We compare them with the common two-dimensional approximations in textbooks. A previously unnoticed pattern in the three-dimensional Foucault pendulum attractor is presented.
N-Alkyl Ammonium Resorcinarene Salts as High-Affinity Tetravalent Chloride Receptors.
2016
N-Alkyl ammonium resorcinarene salts (NARYs, Y=triflate, picrate, nitrate, trifluoroacetates and NARBr) as tetravalent receptors, are shown to have a strong affinity for chlorides. The high affinity for chlorides was confirmed from a multitude of exchange experiments in solution (NMR and UV/Vis), gas phase (mass spectrometry), and solid-state (X-ray crystallography). A new tetra-iodide resorcinarene salt (NARI) was isolated and fully characterized from exchange experiments in the solid-state. Competition experiments with a known monovalent bis-urea receptor (5) with strong affinity for chloride, reveals these receptors to have a much higher affinity for the first two chlorides, a similar af…
Shifting Imageries: Gentrification and the New Touristic Images of the Inner City of Palermo
2021
After the bombings of 1943, the historic centre of Palermo was left with several crumbling buildings and a social situation of some concern. Therefore, over the last sixty years, all kinds of representations of Palermo have revolved around the presence of criminal organizations and the risks of living in the most dangerous areas of the city (including the city centre). These representations started to change around the beginning of the new century when the inner city of Palermo underwent an intense process of redefinition of its territorial identity. Several key factors may be used to explain this process: from traditional economic reasons concerning gentrification and touristification, to …