Search results for "Tert"

La tribù degli antichisti. Un’etnografia ad opera di un suo membro

2006

vengono studiate, antropoologicamente ed epistemologicamente, le categorie e i concetti immpiegati nella scienza dell'antichistà oggi e nella sua storia passata

Philology within the framework of Semiotic Textology. János S. Petőfi’s scientific legacy II

2019

This paper is addressed to linguists and, ideally, to philologists and literary critics. János S. Petőfi formulated his Semiotic Textology within a context of Macro-Disciplines (MD) and within an interdisciplinary context (ID). Petőfi did not give much information about the relationship between Semiotic Textology and other disciplines, such as Philology and Literary Criticism. Textual Linguistics is chronologically the first among the disciplines participating in Natural Text Processing as sub-procedure of Natural Language Processing. On the other hand, Literary Criticism is the last discipline of the procedure, because it expresses artistic evaluation. The connections between the disciplin…

Dissection of the relative contribution of the Schizosaccharomyces pombe Ctr4 and Ctr5 proteins to the copper transport and cell surface delivery fun…

2011

The Ctr1 family of proteins mediates high-affinity copper (Cu) acquisition in eukaryotic organisms. In the fission yeastSchizosaccharomyces pombe, Cu uptake is carried out by a heteromeric complex formed by the Ctr4 and Ctr5 proteins. Unlike human andSaccharomyces cerevisiaeCtr1 proteins, Ctr4 and Ctr5 are unable to function independently in Cu acquisition. Instead, both proteins physically interact with each other to form a Ctr4–Ctr5 heteromeric complex, and are interdependent for secretion to the plasma membrane and Cu transport activity. In this study, we usedS. cerevisiaemutants that are defective in high-affinity Cu uptake to dissect the relative contribution of Ctr4 and Ctr5 to the Cu…

Thermodynamic versus Conformational Metastability in Fibril-Forming Lysozyme Solutions

2012

The role of intermolecular interaction in fibril-forming protein solutions and its relation with molecular conformation is a crucial aspect for the control and inhibition of amyloid structures. Here, we study the fibril formation and the protein-protein interactions of lysozyme at acidic pH and low ionic strength. The amyloid formation occurs after a long lag time and is preceded by the formation of oligomers, which seems to be off-pathway with respect to fibrillation. By measuring the osmotic isothermal compressibility and the collective diffusion coefficient of lysozyme in solution, we observe that the monomeric solution is kept in a thermodynamically metastable state by strong electrosta…

Inhibitory effects of N-acetylcysteine on superoxide anion generation in human polymorphonuclear leukocytes.

1997

Abstract It has been suggested that reactive oxygen species released by activated polymorphonuclear leukocytes (PMN) in man is one mechanism of tissue injury. Therapeutic action aimed at increasing antioxidant defence mechanisms is still a clinical challenge. This study examines the activity of N-acetylcysteine, a known antioxidant, in the protection of PMN exposed in-vitro to the chemoattractant peptide fMet-Leu-Phe (FMLP), the protein kinase C activator phorbol myristate acetate or the lipid peroxidation promoter t-butyl hydroperoxide. FMLP (3–300 nm) and phorbol myristate acetate (160 pm–160 nm) induced concentration-related superoxide anion generation. Pre-treatment with N-acetylcystein…

Glutathione metabolism in skeletal muscle derived cells of the L6 line

1993

Skeletal muscle derived L6 myoblasts possess a considerably high resting total glutathione (TGSH) pool. Exposure to L-buthionine-[S,R]-sulphoximine resulted in a 90% depletion of the intracellular TGSH pool. All the key enzymes of glutathione metabolism, especially glutathione S-transferase, were observed to be considerably active in the undifferentiated cells. Se-dependent glutathione peroxidase activity appeared to account for most of the total GSH peroxidase activity of the cells. A significant contribution of gamma-glutamyl transpeptidase-independent (5 mM acivicin insensitive) mechanism to the extracellular GSH uptake capacity of the muscle cells was evident. Efflux of oxidized glutath…

Mätänevän velhon taidejulistus : intertekstuaalisen ja -figuraalisen aineiston asema Apollinairen L'enchanteur pourrissant teoksen tematiikassa ja sy…

2008

Ranskalainen modernisti Guillaume Apollinaire (1880-1918) marssittaa esikoisteoksessaan L’Enchanteur pourrissant (1909) kuolemattoman velhon haudalle vanhentuneen Troijan Helenan. Rapistuneen kaunottaren ohella mädäntyvän tietäjän lihaa saapuvat haistelemaan mm. viehkeytensä menettäneet juoruileviksi naisiksi muuttuneet haltijattaret, höperöityneet antiikin filosofit ja Raamatun kuolemattomat pyhimykset, jotka ovat unohtaneet uskonsa merkityksen. Apollinairen luomassa groteskissa maailmassa vanha myyttinen perintö kuolee ja mätänee samalla kun maailma vajoaa dekadenttiseen rappioonsa. Riikka Mahlamäki-Kaistisen tutkima Apollinaire on yksi tärkeimmistä 1900-luvun alun ranskalaisista kirjaili…

Guillaume Apollinaire, hymyilevä varas : intertekstuaalisuuden muodot L'Enchanteur pourrissant -teoksessa

2001

Bax-derived membrane-active peptides act as potent and direct inducers of apoptosis in cancer cells.

2011

SUMMARYAlthough many cancer cells are primed for apoptosis, they usually develop resistance to cell death at multiple levels. Permeabilization of the outer mitochondrial membrane, which is mediated by proapoptotic Bcl-2 family members like Bax, is considered as a point-of-no-return for initiating apoptotic cell death. This crucial role has placed Bcl-2 family proteins as recurrent targets for anticancer drug development. Here, we propose and demonstrate a new concept based on using minimal active version of Bax to induce cell death independently of endogenous Bcl-2 proteins. We show that membrane-active segments of Bax can directly induce the release of mitochondria-residing apoptogenic fac…

Regio-specific synthesis of new 1-(tert-butyl)-1H-pyrazolecarboxamide derivatives

2017

Regio-specific and non-regiospecific condensation reactions on 1,3-dicarbonyl compounds rendered 1,3,5-trisubstituted pyrazoles. Herein, the control of regio-specificity was a significant improvement in pyrazole research. A high yield acylation of poorly nucleophilic aryl amines, which resulted in mono- or diacylated products depending on the reaction conditions, is described. As a result, a library of potentially bioactive compounds was obtained. Fil: Ruatta Merke, Santiago Matías. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Murguia, Marcelo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Co…