Search results for "Tetrad"

Shedding of interleukin-6 receptor and tumor necrosis factor alpha. Contribution of the stalk sequence to the cleavage pattern of transmembrane prote…

2000

A functionally and structurally diverse group of transmembrane proteins including transmembrane forms of mediators or receptors can be proteolytically cleaved to form soluble growth factors or receptors. Recently, the proteolytic activity responsible for pro-tumor necrosis factor alpha (proTNFalpha) processing has been identified and named TACE (TNFalpha converting enzyme). In experiments with TACE deficient (TACE-/-) fibroblasts we found that 4beta-phorbol 12-myristate 13-acetate (PMA)-induced shedding of the interleukin-6 receptor (IL-6R) is strongly reduced. A basal hydroxamate sensitive release of IL-6R, however, could still be detected. This result demonstrates that TACE plays a role i…

Wip1 inhibition leads to severe pro-inflammatory phenotype in skin in response to chemical irritation

2016

Signal transduction pathways of membrane expression of proteinase 3 (PR‐3) in human endothelial cells

1997

At present, the exact mechanism of the pathogenic effect of anti-PR-3 antibodies remains unknown. Interaction of anti-neutrophil cytoplasmic antibodies (ANCAs) with human umbilical vein endothelial cells (HUVECs) may play a key role. Recently we were able to show that ANCAs recognize their target antigen, PR-3, translocated into the membrane of HUVECs. The objective of this study was to investigate regulation, i.e. signal transduction pathways, of PR-3 expression in endothelial cells. HUVECs were isolated according to the method of Jaffe et al. and cultured under standard conditions. A cyto-enzyme-linked immunosorbent assay (ELISA) with unfixed cells was performed. Membrane-expressed PR-3 w…

An Aqueous Extract of Ilex paraguariensis Reduces Carrageenan-Induced Edema and Inhibits the Expression of Cyclooxygenase-2 and Inducible Nitric Oxid…

2014

Mate (Ilex paraguariensis) is a highly popular herbal beverage in South America due to its high content of caffeine. Its hypolipidemic and antioxidant properties are of increasing interest in the treatment of cardiovascular disorders and for weight control. In the present study, we show for the first time both the local and systemic anti-inflammatory effects of an aqueous extract of mate in three classic in vivo models, namely acute and chronic 12-O-tetradecanoylphorbol 13-acetate-induced mouse ear edema and acute carrageenan-induced mouse paw edema. Caffeine, rutin, chlorogenic acid, 3,5-dicafeoyl quinic acid, and 4,5-dicafeoyl quinic acid, accompanied by a complex mixture of other simple …

Morphological determination of the phototrophic community composition of biological soil crusts in coastal sand dunes in northern Germany

2022

This dataset comprises the microbial community composition of biological soil crusts in north-German sand dunes. For this we obtained enrichment cultures of phototrophic microorganisms, by placing fragments of biocrusts of the same Petri dishes as used for sequencing, in Petri dishes with Bold Basal (1N BBM) agarized medium (Bischoff and Bold 1963). Cultures were grown under standard laboratory conditions: with a 12-hour alteration of light and dark phases and irradiation of 25 μmol photons m-2 s-1 at a temperature 20 ± 5 ºС. Microscopic study of these raw cultures began in the third week of cultivation. Morphological examinations were performed using Olympus BX53 light microscope with Noma…

The transport activity of the human cationic amino acid transporter hCAT-1 is downregulated by activation of protein kinase C

2001

1 The human cationic amino acid transporter hCAT-1 contains several consensus sequences for phosphorylation by protein kinase C (PKC). This study investigates the eAect of PKC activation on hCAT-1-mediated transport. 2 When expressed in Xenopus laevis oocytes, hCAT-1-mediated L-arginine transport was reduced to 44+3% after a 30 min treatment of the oocytes with 100 nM phorbol-12-myristate-13-acetate (PMA). 4a-phorbol-12,13-didecanoate (4a-PDD, 100 nM) had no eAect. 3 In EA.hy926 endothelial cells, maximal inhibition of hCAT-1-mediated L-arginine transport (to 3‐11% of control) was observed after treatment of the cells with 100 nM PMA for 4 h. A 20‐30 h exposure of the cells to 100 nM PMA le…

In vivo activity of pseudoguaianolide sesquiterpene lactones in acute and chronic inflammation.

2000

The pseudoguaianolide sesquiterpene lactones 4-alpha-O-acetyl-pseudoguaian-6beta-olide (1), hymenin (2), ambrosanolide (3), tetraneurin A (4), parthenin (5), hysterin (6) and confertdiolide (7) were evaluated for their ability to affect the inflammation responses induced by different agents. All the compounds showed activity against the 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced mouse ear edema. The ethyl phenylpropiolate (EPP)-induced mouse ear edema was inhibited by compounds 3, 5 and 7. However, when sesquiterpene-lactones were assayed on the arachidonic acid (AA)-induced mouse ear edema, none of them were active. The only sesquiterpene lactone orally active against the paw mouse…

Synthesis of a New C3-Symmetric Tripodal P4-Tetradentate Ligand and Its Application to the Formation of Chiral Metal Complexes

2010

A novel C3-symmetric tetradentate tripodal ligand with phosphorus as coordinating atoms has been synthesized in good yields. Its coordination ability through the four phosphorus atoms, three at the...

The hot bands of silane between 2120 and 2270cm−1

2005

Abstract The infrared spectrum of the SiH 4 molecule has been recorded between 2040 and 2320 cm −1 using the high-resolution Fourier interferometer of the Laboratoire de Photophysique Moleculaire ( Orsay , France ). The resolution was 5.4 × 10 −3  cm −1 . In this region, many lines were previously analyzed and assigned to the ν 1 / ν 3 stretching dyad of 28 SiH 4 , 29 SiH 4 , and 30 SiH 4 molecules [J. Mol. Spectrosc. 143 (1990) 35]. However, several lines in the spectrum were not assigned. The results obtained in our previous study [J. Mol. Spectrosc. 197 (1999) 307] of the infrared spectrum of 28 SiH 4 , in the bending-stretching tetrad region at 3100 cm −1 , enabled us to assign 204 of t…

Analysis of the Infrared Fourier Transform Spectrum of the Spectra of Silane in the Range 2930-3300 cm(-1).

1999

The infrared spectrum of (28)SiH(4) between 2930 and 3300 cm(-1) was recorded using the Laboratoire de Physique Moleculaire et Applications (LPMA.) Fourier transform spectrometer. The instrumental response function width chosen makes it possible to obtain a Doppler-limited spectrum. The observed spectrum belongs to the tetrad built with one quantum of stretching mode (nu(1) or nu(3)) and one quantum of bending mode (nu(2) or nu(4)). The excited states are formed from eight vibrational sublevels: nu(1) + nu(2)(E), nu(1) + nu(4)(F(2)), nu(2) + nu(3)(F(1) + F(2)), and nu(3) + nu(4)(A(1) + E + F(1) + F(2)). The intricacy of the upper states was resolved using the variations of the line strength…