Search results for "Tipa"
showing 10 items of 596 documents
Potential transbuccal delivery of l-DOPA methylester prodrug: stability in the environment of the oral cavity and ability to cross the mucosal tissue
2016
Levodopa (l-DOPA) is the most effective pharmacologic agent in Parkinson's disease and remains the "gold standard". Nevertheless, in long-term treatments, dyskinesias and motor complications can emerge. In this work, the combined use of l-DOPA methylester hydrochloride prodrug (LDME) with transbuccal drug delivery was supposed as a good alternative method to optimize the bioavailability of l-DOPA, to maintain constant plasma levels and to decrease the drug unwanted effects. The effects of environmental pH on buccal delivery of LDME were evaluated ex vivo. The increase of pH value from 5.8 to 6.2 implies an improvement of drug permeation. Since the pH increase causes the raising of hydrolyti…
Development of a Multiparametric Cell-based Protocol to Screen and Classify the Hepatotoxicity Potential of Drugs
2012
Hepatotoxicity is a major reason for drug nonapprovals and withdrawals. The multiparametric analysis of xenobiotic toxicity at the single cells level using flow cytometry and cellular imaging-based approaches, such as high-content screening (HCS) technology, could play a key role in the detection of toxicity and the classification of compounds based on patterns of cellular injury. This study aimed to develop and validate a practical, reproducible, in vitro multiparametric cell-based protocol to assess those drugs that are potentially hepatotoxic to humans and to suggest their mechanisms of action. The assay was applied to HepG2 human cell line cultured in 96-well plates and exposed to 78 di…
Cytometric analysis for drug-induced steatosis in HepG2 cells
2009
Drugs are capable of inducing hepatic lipid accumulation. When fat accumulates, lipids are primarily stored as triglycerides which results in steatosis and provides substrates for lipid peroxidation. An in vitro multiparametric flow cytometry assay was performed in HepG2 cells by using fluorescent probes to analyze cell viability (propidium iodide, PI), lipid accumulation (BODIPY493/503), mitochondrial membrane potential (tetramethyl rhodamine methyl ester, TMRM) and reactive oxygen species generation (ROS) (2',7'-dihydrochlorofluorescein diacetate, DHCF-DA) as functional markers. All the measurements were restricted to live cells by gating the cells that excluded PI or those that exhibited…
Stability Analysis of Routing Strategies for the Maximum Lifetime Problem in One-Dimensional Ad-Hoc Wireless Networks
2017
In the paper we discuss solutions of the maximum network lifetime problem in one-dimensional, regular ad-hoc wireless networks. For the problem, nodes of the network generate given amount of data and send it possibly via other nodes to the data collector. To extend the network lifetime the data should be transmitted in such a way, that the energy utilized by the most overloaded node is minimized. We assume, that the nodes use the point-to-point data transmission scheme and the cost of transmission of one unit of data is arbitrary superadditive function of a distance between transmitter and receiver. We show that for the one-dimensional network in which the nodes are evenly distributed on th…
A Branch-and-Cut method for the Capacitated Location-Routing Problem
2011
International audience; Recent researches in the design of logistic networks have shown that the overall distribution cost may be excessive if routing decisions are ignored when locating depots. The Location-Routing Problem (LRP) overcomes this drawback by simultaneously tackling location and routing decisions. The aim of this paper is to propose an exact approach based on a Branch-and-Cut algorithm for solving the LRP with capacity constraints on depots and vehicles. The proposed method is based on a zero-one linear model strengthened by new families of valid inequalities. The computational evaluation on three sets of instances (34 instances in total), with 5–10 potential depots and 20–88 …
TBRA: A scalable routing algorithm in highly mobile large scale pure ad hoc wireless mesh networks
2009
In highly mobile pure ad-hoc wireless mesh networks, fast rerouting within low routing discovery delay is a mandatory requirement for routing algorithm to support interactive applications such as VoIP. Also, the communication overhead should be thwarted when networks grow to a large scale. We propose a Tree-Based Routing Algorithm - TBRA to facilitate such two goals. On one hand, TBRA has very low routing discovery delay due to its proactive property. On the other hand, TBRA performs more efficiently than other proactive protocols with respect to low communication overhead, which thanks to its short routing packet length, less numbers and smaller routing table size. Our modeling analysis an…
A flexible and tunable route discovery mechanism for on-demand protocols
2004
Mobile ad-hoc networks are characterized by multihop wireless links and absence of cellular infrastructure. For the last years, routing has been the area under most intensive research. In particular, on-demand routing protocols proposed are extremely attractive due to their low-overhead and efficiency. Currently, wireless ad-hoc networks lack multipath routing protocols. Multipath routing enables increased QoS support, load balancing and enhanced route stability. In this paper we present a new route discovery solution that increases the number of routes found, while reducing the routing overhead when compared to previous proposals. Simulation results show that the additional routing overhea…
Logic-Based Distributed Routing for NoCs
2008
The design of scalable and reliable interconnection networks for multicore chips (NoCs) introduces new design constraints like power consumption, area, and ultra low latencies. Although 2D meshes are usually proposed for NoCs, heterogeneous cores, manufacturing defects, hard failures, and chip virtualization may lead to irregular topologies. In this context, efficient routing becomes a challenge. Although switches can be easily configured to support most routing algorithms and topologies by using routing tables, this solution does not scale in terms of latency and area. We propose a new circuit that removes the need for using routing tables. The new mechanism, referred to as logic-based dis…
Both Short- and Long-Acting D-1/D-2 Dopamine Agonists Induce Less Dyskinesia than l-DOPA in the MPTP-Lesioned Common Marmoset (Callithrix jacchus)
2002
Abstract The current concept of dyskinesia is that pulsatile stimulation of D-1 or D-2 receptors by l -DOPA or short-acting dopamine agonists is more likely to induce dyskinesia compared to long-acting drugs producing more continuous receptor stimulation. We now investigate the ability of two mixed D-1/D-2 agonists, namely pergolide (long-acting) and apomorphine (short-acting), to induce dyskinesia in drug-naive MPTP-lesioned primates, compared to l -DOPA. Adult common marmosets ( Callithrix jacchus ) were lesioned with MPTP (2 mg/kg/day sc for 5 days) and subsequently treated with equieffective antiparkinsonian doses of l -DOPA, apomorphine, or pergolide for 28 days. l -DOPA, apomorphine, …
Intrinsic competition between two European egg parasitoids of the brown marmorated stink bug
2020
Following the accidental introduction and spread of the invasive polyphagous agricultural pest Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), the two European egg parasitoids Anastatus bifasciatus (Geoffroy) (Hymenoptera: Eupelmidae) and Ooencyrtus telenomicida (Vassiliev) (Hymenoptera: Encyrtidae) have been investigated for inundative biological control. Since the competititve outcome between the two generalist parasitoids is difficult to predict, intrinsic competition was investigated with a time-course development study. Both species readily oviposited in H. halys eggs containing eggs and early instar larvae of the competitor, but oviposition decreased when eggs contained late insta…