Search results for "Toxicity"

JA47, a new histone deacetylase inhibitor that induces cytotoxic effects on triple-negative MDA-MB231 breast cancer cells in vitro

2012

Metallic nanoparticles exhibit paradoxical effects on oxidative stress and pro-inflammatory response in endothelial cells in vitro

2007

Particulate matter is associated with different human diseases affecting organs such as the respiratory and cardiovascular systems. Very small particles (nanoparticles) have been shown to be rapidly internalized into the body. Since the sites of internalization and the location of the detected particles are often far apart, a distribution via the blood stream must have occurred. Thus, endothelial cells, which line the inner surface of blood vessels, must have had direct contact with the particles. In this study we tested the effects of metallic nanoparticles (Co and Ni) on oxidative stress and proinflammatory response in human endothelial cells in vitro. Exposure to both nanoparticle types…

Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells.

2019

Background: The aim of this study was: to evaluate the biological properties of new hydraulic materials: Bio-C Repair and Bio-C Sealer. Methods: Periodontal ligament stem cells were exposed to several dilutions of Bio-C Repair and Bio-C Sealer. The ion release profile and pH were determined. Metabolic activity, cell migration and cell survival were assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), wound-healing assays and Annexin assays, respectively. Cells were cultured in direct contact with the surface of each material. These were then analyzed via scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Statistical differences were ass…

Immunotoxicity of metal nanoparticles in Eisenia andrei earthworms

2022

En los últimos años, la producción de nanopartículas (NPs) ha aumentado dando lugar a que su liberación al medio ambiente haya incrementado. Tanto la liberación ambiental como su desecho se está convirtiendo en una preocupación emergente ya que las NPs alcanzarían los ecosistemas terrestres como acuáticos, presentando problemas de toxicidad para los organismos vivos. En los ecosistemas terrestres, las lombrices de tierra Eisenia andrei son invertebrados típicamente utilizados como organismos modelo en estudios de ecotoxicidad con un sistema inmunológico innato que protege a los animales contra contaminantes, bacterias, hongos y virus que se encuentran en el suelo. El objetivo general de est…

Effect of cadmium pre-exposure in cadmium accumulation by brine shrimp Artemia: Involvement of low-molecular-weight cadmium-binding ligands

1993

Abstract Cadmium is a ubiquitous non-essential element that possesses high toxicity and is easily accumulated from the environment by aquatic organisms. It is known that pre-exposure to cadmium increases the resistance of animals to a subsequent exposure. In this work, we report studies of the effect of pre-exposure to cadmium on the accumulation of this metal from seawater by brine shrimp Artemia, as well as the involvement of low-molecular-weight cadmium-binding ligands in this process. During the pre-exposure experiments, animals were kept in seawater containing 20 mg/litre Cd for 24 h and then transferred to clean seawater for 48 h. Afterwards, pre-exposed and control animals were expos…

Fe and Zn co-substituted beta-tricalcium phosphate (β-TCP): Synthesis, structural, magnetic, mechanical and biological properties

2020

This work was supported by the European Social Fund under the No. 09.3.3- LMT-K-712 “Development of Competences of Scientists, other Researchers and Students through Practical Research Activities” measure. AK would like to express sincere gratitude for Fellowship administrated by The Japan Society for the Promotion of Science (JSPS). Fellow’s ID No.: L12546. Authors are grateful to R. Vargalis (Vilnius University) for taking SEM images. © 2020. This work is licensed under a CC BY-NC-ND license.

Bioavailability of inorganic arsenic in cooked rice: practical aspects for human health risk assessments.

2005

Arsenic is present in rice grain mainly as inorganic arsenic. Little is known about the effect of cooking on inorganic arsenic content in rice and its bioavailability. This study evaluated total arsenic and inorganic arsenic in rice cooked with arsenic-contaminated water, the bioaccessibility of As(III) and As(V) after simulated gastrointestinal digestion, and the extent of arsenic retention and transport by Caco-2 cells used as a model of intestinal epithelia. After cooking, inorganic arsenic contents increase significantly. After simulated gastrointestinal digestion, the bioaccessibility of inorganic arsenic reached 63-99%; As(V) was the main species found. In Caco-2 cells, arsenic retent…

Defective copper transport in the copt5 mutant affects cadmium tolerance.

2014

Cadmium toxicity interferes with essential metal homeostasis, which is a problem for both plant nutrition and the consumption of healthy food by humans. Copper uptake is performed by the members of the Arabidopsis high affinity copper transporter (COPT) family. One of the members, COPT5, is involved in copper recycling from the vacuole toward the cytosolic compartment. We show herein that copt5 mutants are more sensitive to cadmium stress than wild-type plants, as indicated by reduced growth. Exacerbated cadmium toxicity in copt5 mutants is due specifically to altered copper traffic through the COPT5 transporter. Three different processes which have been shown to affect cadmium tolerance ar…

Effect of cadmium exposure on zinc levels in the brine shrimp Artemia parthenogenetica

1999

Zinc and cadmium have been reported as metabolic antagonists, such that high zinc intake afford animals some protection against the potentially toxic effects of cadmium exposure. There is considerable evidence to support a role of metallothioneins (MTs) in regulating or controlling the intracellular availability of essential metals and the non-essential metal. The effect of 24-h cadmium pre-exposure (10 mg Cd/l) on zinc concentrations in the brine shrimp Artemia parthenogenetica exposed to zinc (5 mg Zn/l) was studied. The zinc content of shrimps was not altered by cadmium. The homeostatic mechanism for zinc regulation appears not to be disturbed by cadmium exposure in shrimps kept in natur…

Diagnosis of boron, fluorine, lead, nickel and zinc toxicity in citrus plantations in Villarreal, Spain

1997

In the late 1980s, citrus plantations in the area of Villarreal (Spain) showed injuries similar to those previously reported for boron and fluorine toxicity. The area was affected by the disposal of industrial wastewater, mainly from ceramic industries. Conjunctive uses of surface water, groundwater and wastewater for irrigation had taken place. A survey was conducted at 25 orchards to assess leaves and soil for their boron, fluorine, lead, nickel and zinc contents. Wastewater and groundwater were also analyzed to corroborate the presence of these pollutants. The results showed that both boron and fluorine contents were greater than those reported as excess at the most part of the orchards …