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RESEARCH PRODUCT

Metallic nanoparticles exhibit paradoxical effects on oxidative stress and pro-inflammatory response in endothelial cells in vitro

Roman TsarykE SabbioniAntonietta GattiKirsten PetersC.j. KirkpatrickRonald E. Unger

subject

human diseasesmedia_common.quotation_subjectImmunologyNanoparticleCell CountInflammationBiologymedicine.disease_cause03 medical and health sciences0302 clinical medicineMicroscopy Electron TransmissionNickelmedicineHumansImmunology and AllergyParticle SizeCytotoxicityInternalizationCells CulturedChemokine CCL2media_commonInflammationPharmacologyInterleukin-8Endothelial CellsCobaltIntercellular Adhesion Molecule-1GlutathioneIn vitroCell biologyOxidative StressMetalsNanotoxicology030220 oncology & carcinogenesisNanoparticlesGentian Violetmedicine.symptomSignal transductionReactive Oxygen SpeciesOxidation-ReductionOxidative stress030215 immunology

description

Particulate matter is associated with different human diseases affecting organs such as the respiratory and cardiovascular systems. Very small particles (nanoparticles) have been shown to be rapidly internalized into the body. Since the sites of internalization and the location of the detected particles are often far apart, a distribution via the blood stream must have occurred. Thus, endothelial cells, which line the inner surface of blood vessels, must have had direct contact with the particles. In this study we tested the effects of metallic nanoparticles (Co and Ni) on oxidative stress and proinflammatory response in human endothelial cells in vitro. Exposure to both nanoparticle types led to a concentration-dependent cytotoxic effect. However, the effects on oxidative stress and pro-inflammatory response differed dramatically. Due to the nanoparticle-induced effects, a comparison between metallic nanoparticle- and metal ion-treatment with the corresponding ions was made. Again, divergent effects of nanoparticles compared with the ions were observed, thus indicating differences in the signaling pathways induced by these compounds. These paradoxical responses to different metallic nanoparticles and ions demonstrate the complexity of nanoparticle-induced effects and suggest the need to design new strategies for nanoparticle toxicology.

10.1177/039463200702000404https://hdl.handle.net/11380/612239