Search results for "Transcription"
showing 10 items of 2278 documents
Polar Localization of a Tripartite Complex of the Two-Component System DcuS/DcuR and the Transporter DctA in Escherichia coli Depends on the Sensor K…
2014
The C4-dicarboxylate responsive sensor kinase DcuS of the DcuS/DcuR two-component system of E. coli is membrane-bound and reveals a polar localization. DcuS uses the C4-dicarboxylate transporter DctA as a co-regulator forming DctA/DcuS sensor units. Here it is shown by fluorescence microscopy with fusion proteins that DcuS has a dynamic and preferential polar localization, even at very low expression levels. Single assemblies of DcuS had high mobility in fast time lapse acquisitions, and fast recovery in FRAP experiments, excluding polar accumulation due to aggregation. DctA and DcuR fused to derivatives of the YFP protein are dispersed in the membrane or in the cytosol, respectively, when …
Networks of Seed Storage Protein Regulation in Cereals and Legumes at the Dawn of the Omics Era
2012
BAP GEAPSI CT2 (BAP); International audience; Finely regulated and orchestrated events occur during seed development and germination in different cell organelles (mitochondria, peroxisomes, plastids, vacuoles, endoplasmic reticulum, oil bodies and nuclei). Therefore, some of the ongoing investigations pass through sub-cellular organelle purifications for a deeper comprehension of these complex developmental processes. In seed biology, recent ambitious efforts in proteomics have been directed toward organelle isolation from seeds and high-throughput protein separation and identification by mass spectrometry at defined developmental stages. The construction of reference maps allowed identifyi…
The roles of the embryo-surrounding tissues in regulating Medicago truncatula seed filling
2011
National audience
Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress
2013
Sulphur is an essential macronutrient for plant growth, development and response to various abiotic and biotic stresses due to its key role in the biosynthesis of many S-containing compounds. Sulphate represents a very small portion of soil S pull and it is the only form that plant roots can uptake and mobilize through H(+)-dependent co-transport processes implying sulphate transporters. Unlike the other organically bound forms of S, sulphate is normally leached from soils due to its solubility in water, thus reducing its availability to plants. Although our knowledge of plant sulphate transporters has been growing significantly in the past decades, little is still known about the effect of…
Changing European storm loss potentials under modified climate conditions according to ensemble simulations of the ECHAM5/MPI-OM1 GCM
2007
Abstract. A simple storm loss model is applied to an ensemble of ECHAM5/MPI-OM1 GCM simulations in order to estimate changes of insured loss potentials over Europe in the 21st century. Losses are computed based on the daily maximum wind speed for each grid point. The calibration of the loss model is performed using wind data from the ERA40-Reanalysis and German loss data. The obtained annual losses for the present climate conditions (20C, three realisations) reproduce the statistical features of the historical insurance loss data for Germany. The climate change experiments correspond to the SRES-Scenarios A1B and A2, and for each of them three realisations are considered. On average, insure…
Rainfall variability in subequatorial America and Africa and relationships with the main sea-surface temperature modes (1951–1990)
1995
The rainfall variability of subequatorial South America and Africa is poorly documented owing to the scarcity of data. We present a new land-only data set of monthly precipitation from 1951 to 1990, focusing on subequatorial South America and Africa, which improves the knowledge of rainfall variability and allows comparisons with GCM outputs. The results of multivariate analyses are compared with those performed on the best actual global rainfall data set developed by Mike Hulme. The main modes of bimonthly rainfall variability are not located in the major rain-forest basins of Za'ke and Amazonia, but rather on the tropical margins, such as Venezuela or Sudan, and near-coastal equatorial ar…
Inactivation of PadR, the repressor of the phenolic acid stress response, by molecular interaction with Usp1, a universal stress protein from Lactoba…
2009
ABSTRACT The phenolic acid decarboxylase gene padA is involved in the phenolic acid stress response (PASR) in gram-positive bacteria. In Lactobacillus plantarum , the padR gene encodes the negative transcriptional regulator of padA and is cotranscribed with a downstream gene, usp1 , which encodes a putative universal stress protein (USP), Usp1, of unknown function. The usp1 gene is overexpressed during the PASR. However, the role and the mechanism of action of the USPs are unknown in gram-positive bacteria. Therefore, to gain insights into the role of USPs in the PASR; (i) a usp1 deletion mutant was constructed; (ii) the two genes padR and usp1 were coexpressed with padA under its own promo…
Persistance and adaptation of Listeria monocytogenes in the soil : role of the communication system Agr
2014
Listeria monocytogenes is a ubiquitous bacterium responsible for listeriosis, a food-borne disease. This pathogen has been isolated from various environments of which the telluric environment. The presence of L. monocytogenes in soil can increase health hazards due to the risk of transfer to vegetables, animals and animal products and water. Considering the role of soil in the circulation of pathogens from farm environment to plant and animal products and eventually to foodstuff, it is critical to identify intrinsic and extrinsic factors that drive the fate of L. monocytogenes in soil. Genome-wide and transcriptomic analyses found that an important part of the genome of L. monocytogenes (7.…
The Transcription Factor Promyelocytic Leukemia Zinc Finger Protein Is Associated With Expression of Liver‐Homing Receptors on Human Blood CD56bright…
2020
The transcription factor promyelocytic leukemia zinc finger protein (PLZF) is involved in the development of natural killer (NK) cells and innate lymphoid cells, including liver‐resident NK cells in mice. In human NK cells, the role of PLZF in liver residency is still unknown. Expression of PLZF in matched human peripheral blood‐ and liver‐derived NK cells and the association of PLZF expression with surface molecules and transcription factors relevant for tissue residency were investigated using multiparameter flow cytometry and assessing single‐cell messenger RNA (mRNA) levels. Intrahepatic cluster of differentiation (CD)56bright NK cells expressed significantly higher levels of PLZF than …
Etude de la régulation du facteur de transcription ATB2 chez Medicago truncatula
2013
Rapport de stage de Master 2 Spécialité Biologie Intégrative des Interactions Plantes, Microbes, Environnement BAP GEAPSI; Master; Climatic changes since more than a century combined with the urge for better crop yields due to a huge demographic growth and coupled with the need for a more sustainable agriculture led to a number of stresses for plants. Understanding them and their effect on growth, seed quality and seed production of plants is one of the most important agronomical issues of our time. We have chosen to study legumes because they are a major lipid and protein source, as both food and feed, and for their unique ability to absorb the atmospheric nitrogen via a symbiosis with Rhi…