Search results for "Transgene"
showing 9 items of 259 documents
Vaccination with ENO1 DNA Prolongs Survival of Genetically Engineered Mice with Pancreatic Cancer
2013
Background & Aims Pancreatic ductal adenocarcinoma (PDA) is an aggressive tumor, and patients typically present with late-stage disease; rates of 5-year survival after pancreaticoduodenectomy are low. Antibodies against α-enolase (ENO1), a glycolytic enzyme, are detected in more than 60% of patients with PDA, and ENO1-specific T cells inhibit the growth of human pancreatic xenograft tumors in mice. We investigated whether an ENO1 DNA vaccine elicits antitumor immune responses and prolongs survival of mice that spontaneously develop autochthonous, lethal pancreatic carcinomas. Methods We injected and electroporated a plasmid encoding ENO1 (or a control plasmid) into Kras G12D /Cre (KC) mice …
Przeciw mitom - o mrocznych stronach macierzyństwa w powieści Slavenki Drakulić - "Optužena"
2017
Starting with Jean Jacob Rousseau’s, Sigmund Freud’s and feminists’ of the second wave views on matemity, the paper shows Slavenka Drakulić’s ideas presented in her novel Optužena. The Croatian author rejects the myth of matemal love by writing about yiolence against the child and pathology in the mother-daughter relationship. The problem of transgenerational transmission of trauma is shown in an interesting literary way and it is really innovatory.
O materinstvu na drugi način - prećutane strane materinstva u romanu "Optužena" Slavenke Drakulić
2017
Slavenka Drakulić is a Croatian journalist and writer, known as a feminist who writes about the problems in women’s lives. In the novel Optužena (2012), she demonstrates in an interesting, artistic way the less known questions of motherhood, often ignored and tabooed in social discourse. It is known that starting from Jean-Jacques Rousseau’s ideas about maternity, it has been idealized and used in order to constrain women within the patriarchal system, by identifying them primarily as mothers. The concepts of Sigmund Freud, who believed maternity and maternal love to be natural and appropriate for every “standard” woman, contributed to turning into taboos some pathologies connected with mat…
2016
Objectives Human leukocyte antigen (HLA)-B27 (B27) is the strongest genetic factor associated with development of Ankylosing Spondylitis and other spondyloarthropathies (SpA), yet the role it plays in disease pathogenesis remains unclear. We investigated the expression of potentially pathogenic non-conventional heavy chain forms (NC) of B27 in synovial and intestinal tissues obtained from SpA patients. We also determined the presence of NC-B27 in joints, lymphoid and gastrointestinal tissue from B27 transgenic (TG1) rats with M.tuberculosis-induced SpA.
Induction of body weight loss through RNAi-knockdown of APOBEC1 gene expression in transgenic rabbits
2014
In the search of new strategies to fight against obesity, we targeted a gene pathway involved in energy uptake. We have thus investigated the APOB mRNA editing protein (APOBEC1) gene pathway that is involved in fat absorption in the intestine. The APOB gene encodes two proteins, APOB100 and APOB48, via the editing of a single nucleotide in the APOB mRNA by the APOBEC1 enzyme. The APOB48 protein is mandatory for the synthesis of chylomicrons by intestinal cells to transport dietary lipids and cholesterol. We produced transgenic rabbits expressing permanently and ubiquitously a small hairpin RNA targeting the rabbit APOBEC1 mRNA. These rabbits exhibited a moderately but significantly reduced …
Prenatalny okres życia człowieka
2020
Rozwój człowieka w prenatalnym okresie życia interesował ludzi już w czasach starożytności. I choć do dziś prowadzi się wiele dyskusji na temat początku ludzkiego życia, należy stwierdzić, że rozwój poczętego dziecka oraz jego kompetencje wskazują na to, że od początku swojego istnienia jest to osoba aktywna i samosterowalna, która czuje, zapamiętuje, uczy się, posiada pewien poziom świadomości. Radzi sobie z trudnościami hamującymi jego rozwój, przystosowuje do zmiennych warunków życia nie tylko wewnątrz-, ale i zewnątrzmacicznego, nabywa pewnych nawyków i przyzwyczajeń, reaguje w indywidualny, charakterystyczny dla siebie sposób
Estudio del trauma y su vínculo con los sueños de pacientes en tratamiento psicológico. Una visión transgeneracional
2019
El concepto de trauma y su modo de tratarlo desde una adecuada conceptualización clínica, ocupa hoy día una posición central en el diálogo científico interdisciplinar, mostrando la psicología un notable interés por este trastorno. Existe una dificultad clínica en el diagnóstico del trauma, por estar desconectados de la sintomatología, provocar un bloqueo emocional y generar disfunciones en los principales procesos psicológicos como la percepción, el pensamiento, los sentimientos, la imaginación, la memoria y la función onírica de los pacientes clínicos. Generalmente a la persona le afectan varios traumas de diferentes etapas de su vida, además de poder influirle acontecimientos sufridos por…
Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery
2004
Recombinant baculoviruses have been extensively used as vectors for abundant expression of a large variety of foreign proteins in insect cell cultures. The appeal of the system lies essentially in easy cloning techniques and virus propagation combined with the eukaryotic post-translational modification machinery of the insect cell. Recently, a novel molecular biology tool was established by the development of baculovirus surface display, using different strategies for presentation of foreign peptides and proteins on the surface of budded virions. This eukaryotic display system enables presentation of large complex proteins on the surface of baculovirus particles and has thereby become a ver…
Tumor targeting of baculovirus displaying a lymphatic homing peptide.
2008
Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…