Search results for "Type I"

showing 10 items of 966 documents

Collagen-induced differential expression of an RNA polymerase subunit by breast cancer cells

2005

It was previously reported that the stroma of ductal infiltrating carcinoma (DIC) of the human breast contains considerable amount of an embryo-foetal collagen type, OF/LB (onco-foetal/laminin-binding), and that adhesion of 8701-BC DIC cells onto OF/LB collagen substrates selectively promotes cell growth, motility, production of extracellular lytic enzymes and invasion "in vitro" if compared with other collagen species. To detect possible transcriptional differences for regulatory proteins following OF/LB collagen-cell interactions, we submitted RNA preparations from 8701-BC cells grown on collagen type I, IV and OF/LB to "differential display"-PCR in the presence of degenerate C(2)H(2) zin…

Collagen Type IVProtein subunitBreast NeoplasmsBiologyPolymerase Chain ReactionBiochemistryCollagen Type Ichemistry.chemical_compoundBreast cancerRNA polymeraseRNA Ribosomal 18STumor Cells CulturedExtracellularHumansSettore BIO/06 - Anatomia Comparata E CitologiaGeneCell growthRNACell DifferentiationGeneral MedicineMolecular biologyUp-RegulationEnzyme ActivationGene Expression Regulation NeoplasticProtein SubunitschemistryCell cultureRNA polymeraseFemaleLamininRNA Polymerase IICollagenCell cultureGlyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)Tyrosine kinase
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Full‐thickness tissue engineered oral mucosa for genitourinary reconstruction: A comparison of different collagen‐based biodegradable membranes

2020

Tissue engineering is a method of growing importance regarding clinical application in the genitourinary region. One of the key factors in successfully development of an artificially tissue engineered mucosa equivalent (TEOM) is the optimal choice of the scaffold. Collagen scaffolds are regarded as gold standard in dermal tissue reconstruction. Four distinct collagen scaffolds were evaluated for the ability to support the development of an organotypical tissue architecture. TEOMs were established by seeding cocultures of primary oral epithelial cells and fibroblasts on four distinct collagen membranes. Cell viability was assessed by MTT-assay. The 3D architecture and functionality of the ti…

Collagen Type IVScaffoldMaterials scienceSwineBiomedical EngineeringTenascinBiocompatible MaterialsMatrix (biology)Fibroblast migrationBiomaterials03 medical and health sciences0302 clinical medicineTissue engineeringAbsorbable ImplantsMaterials TestingmedicineAnimalsViability assayOral mucosaFibroblastCells CulturedTissue EngineeringTissue ScaffoldsbiologyKeratin-13Mouth MucosaEpithelial CellsMembranes ArtificialTenascin030206 dentistryFibroblastsPlastic Surgery ProceduresCoculture TechniquesUrogenital Surgical ProceduresCell biologymedicine.anatomical_structure030220 oncology & carcinogenesisbiology.proteinJournal of Biomedical Materials Research Part B: Applied Biomaterials
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Characterization and Expression of Multiple Alternatively Spliced Transcripts of the Goodpasture Antigen Gene Region. Goodpasture Antibodies Recogniz…

1995

Collagen IV, the major component of basement membranes, is composed of six distinct alpha chains (alpha 1-alpha 6). Atypically among the collagen IV genes, the exons encoding the carboxyl-terminal region of the human alpha 3(IV) chain undergo alternative splicing. This region has been designated as the Goodpasture antigen because of its reactivity in the kidney and lung with the pathogenic autoantibodies causing Goodpasture syndrome. The data presented in this report demonstrate that, in human kidney, the gene region encompassing the Goodpasture antigen generates at least six alternatively spliced transcripts predicting five distinct proteins that differ in their carboxyl-terminus and retai…

Collagen Type IVTranscription GeneticAnti-Glomerular Basement Membrane DiseaseMolecular Sequence DataGene ExpressionBiologyAutoantigensPolymerase Chain ReactionBiochemistrylaw.inventionMiceExonAntigenIn vivolawmedicineAnimalsHumansGoodpasture syndromeAmino Acid SequenceRNA MessengerGeneAutoantibodiesDNA PrimersMice Inbred BALB CBase SequenceAlternative splicingAutoantibodymedicine.diseaseMolecular biologyRecombinant ProteinsAlternative SplicingRecombinant DNAbiology.proteinCollagenAntibodyEuropean Journal of Biochemistry
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Regulation of type IV collagen gene expression and degradation in fast and slow muscles during dexamethasone treatment and exercise.

2003

Glucocorticoids have anti-anabolic effects on many tissues and can cause muscle atrophy. However, their effects on type IV collagen gene expression and degradation in skeletal muscle have not been studied previously. Rats were treated daily with dexamethasone or saline. Half the groups of experimental and control animals were also subjected to daily endurance or uphill running exercise to determine the possible preventive effects of exercise. After an experimental period of 3 or 10 days, the extensor digitorum longus, soleus and tibialis anterior muscles were studied. Dexamethasone treatment for 10 days reduced muscle weight and type IV collagen mRNA abundance in all muscles. Gene expressio…

Collagen Type IVmedicine.medical_specialtyPhysiologyClinical BiochemistryAnti-Inflammatory AgentsRadioimmunoassayMatrix metalloproteinaseDexamethasoneRats Sprague-DawleyType IV collagenPhysiology (medical)Internal medicinePhysical Conditioning AnimalGene expressionmedicineAnimalsRNA MessengerReceptorMuscle SkeletalGlucocorticoidsDexamethasoneRegulation of gene expressionTissue Inhibitor of Metalloproteinase-2ChemistrySkeletal muscleBlotting NorthernMuscle atrophyRatsEndocrinologymedicine.anatomical_structureMuscle Fibers Slow-TwitchGene Expression RegulationMuscle Fibers Fast-TwitchMatrix Metalloproteinase 2Femalemedicine.symptommedicine.drugPflugers Archiv : European journal of physiology
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Epithelial NEMO links innate immunity to chronic intestinal inflammation

2007

Deregulation of intestinal immune responses seems to have a principal function in the pathogenesis of inflammatory bowel disease(1-4). The gut epithelium is critically involved in the maintenance of intestinal immune homeostasis-acting as a physical barrier separating luminal bacteria and immune cells, and also expressing antimicrobial peptides(3,5,6). However, the molecular mechanisms that control this function of gut epithelial cells are poorly understood. Here we show that the transcription factor NF kappa B, a master regulator of pro-inflammatory responses(7,8), functions in gut epithelial cells to control epithelial integrity and the interaction between the mucosal immune system and gu…

ColonAntimicrobial peptidesApoptosisBiologyPathogenesisInterleukin 22MiceImmune systemAnimalsHomeostasisMultidisciplinaryInnate immune systemNF-kappa BEpithelial CellsColitisImmunity InnateI-kappa B KinaseGut EpitheliumCell biologyIntestinesReceptors Tumor Necrosis Factor Type IChronic DiseaseMyeloid Differentiation Factor 88Tumor Necrosis FactorsImmunologyChronic inflammatory responseTumor necrosis factor alphaSignal TransductionNature
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KNOTS WITH UNKNOTTING NUMBER ONE AND GENERALISED CASSON INVARIANT

1996

We extend the classical notion of unknotting operation to include operations on rational tangles. We recall the “classical” conditions (on the signature, linking form etc.) for a knot to have integral (respectively rational) unknotting number one. We show that the generalised Casson invariant of the twofold branched cover of the knot gives a further necessary condition. We apply these results to some Montesinos knots and to knots with less than nine crossings.

CombinatoricsAlgebra and Number TheoryKnot (unit)Unknotting numberMathematics::Geometric TopologyCasson invariantMathematicsKnot theoryFinite type invariantJournal of Knot Theory and Its Ramifications
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On orderability of fibred knot groups

2003

It is known that knot groups are right-orderable, and that many of them are not bi-orderable. Here we show that certain bred knots in S 3 (or in a homology sphere) do have bi-orderable fundamental group. In particular, this holds for bred knots, such as 41, for which the Alexander polynomial has all roots real and positive. This is an application of the construction of orderings of groups, which are moreover invariant with respect to a certain automorphism.

CombinatoricsAlgebraHOMFLY polynomialKnot invariantGeneral MathematicsSkein relationAlexander polynomialKnot polynomialTricolorabilityMathematics::Geometric TopologyMathematicsKnot theoryFinite type invariantMathematical Proceedings of the Cambridge Philosophical Society
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Enzymatic alteration of C1q, the collagen-like subcomponent of the first component of complement, leads to cross-reactivity with type II collagen

1988

AbstractNative serum C1q, the collagenous-like subcomponent of the first component of complement, is not recognized by polyclonal anti-collagen type II antibodies. However, when purified C1q was subjected to limited proteolysis by collagenase it showed antigenic cross-reactivity with collagen type II. The same cross-reactivity was observed with hemolytically active C1q in synovial fluids of patients with rheumatoid arthritis (RA), whereas C1q from synovial fluids of patients with osteoarthritis (OA), villo-nodular synovitis and ankylosing spondylitis was not recognized by this antibody. However, incubation of synovial fluid C1q of OA patients with synovial fluid leucocytes from RA patients …

Complement Activating EnzymesCollagenaseComplementBiophysicsType II collagenEnzyme-Linked Immunosorbent Assaychemical and pharmacologic phenomenaOsteoarthritisBiochemistryAntibodiesArthritis Rheumatoidfluids and secretionsAntigenComplement C1immune system diseasesStructural BiologySynovitisOsteoarthritisSynovial FluidGeneticsmedicineAnimalsHumansSynovial fluidSpondylitis AnkylosingAntigensRheumatoid arthritisskin and connective tissue diseasesMolecular BiologyC1qAutoantibodiesSheepSynovitisbiologyChemistryComplement C1qAntibodies MonoclonalCell Biologymedicine.diseaseMolecular biologyMicrobial CollagenasePolyclonal antibodiesImmunologyCollagenasebiology.proteinCollagenAntibodyGranulocytesmedicine.drugFEBS Letters
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New expressions for string loop amplitudes leading to an ultrasimple conception of string dynamics

1991

New expressions are derived for string loop amplitudes as overlap integrals of string wave functionals. They are shown to take the form of exchange terms coming from the Bose-Einstein symmetrization between string segments. One is thus led to the ultrasimple conception that string theory is basically free, and that ``string interactions'' are merely due to the fact that strings are composite objects with Bose-Einstein segments as constituents.

Condensed Matter::Quantum GasesPhysicsFísicaString field theoryType I string theoryString theoryRelationship between string theory and quantum field theoryHigh Energy Physics::TheoryDomain wall (string theory)Non-critical string theoryClassical mechanicsSymmetrizationString dualityPhysical Review D
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Fermi-type interaction in molecular and atomic Hamiltonians. Application to molecular systems and Bose-Einstein condensates.

2008

International audience; We present a simple prescription to build phenomenological Hamiltonians describing Fermi-type interactions and apply the developed formalism to two distinct physical systems. First, in a very simple way, we derive equations describing time dynamics of two coherently coupled Bose-Einstein condensates. Further, for bent XY2 molecules, we reproduce all the experimental data with an excellent precision.

Condensed Matter::Quantum Gases[ PHYS.QPHY ] Physics [physics]/Quantum Physics [quant-ph]Fermi-type Interaction. XY2. BEC[PHYS.QPHY]Physics [physics]/Quantum Physics [quant-ph][PHYS.QPHY] Physics [physics]/Quantum Physics [quant-ph]
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