6533b821fe1ef96bd127ba06
RESEARCH PRODUCT
Enzymatic alteration of C1q, the collagen-like subcomponent of the first component of complement, leads to cross-reactivity with type II collagen
Dieter BrackertzMichael LoosHans-peter Heinzsubject
Complement Activating EnzymesCollagenaseComplementBiophysicsType II collagenEnzyme-Linked Immunosorbent Assaychemical and pharmacologic phenomenaOsteoarthritisBiochemistryAntibodiesArthritis Rheumatoidfluids and secretionsAntigenComplement C1immune system diseasesStructural BiologySynovitisOsteoarthritisSynovial FluidGeneticsmedicineAnimalsHumansSynovial fluidSpondylitis AnkylosingAntigensRheumatoid arthritisskin and connective tissue diseasesMolecular BiologyC1qAutoantibodiesSheepSynovitisbiologyChemistryComplement C1qAntibodies MonoclonalCell Biologymedicine.diseaseMolecular biologyMicrobial CollagenasePolyclonal antibodiesImmunologyCollagenasebiology.proteinCollagenAntibodyGranulocytesmedicine.drugdescription
AbstractNative serum C1q, the collagenous-like subcomponent of the first component of complement, is not recognized by polyclonal anti-collagen type II antibodies. However, when purified C1q was subjected to limited proteolysis by collagenase it showed antigenic cross-reactivity with collagen type II. The same cross-reactivity was observed with hemolytically active C1q in synovial fluids of patients with rheumatoid arthritis (RA), whereas C1q from synovial fluids of patients with osteoarthritis (OA), villo-nodular synovitis and ankylosing spondylitis was not recognized by this antibody. However, incubation of synovial fluid C1q of OA patients with synovial fluid leucocytes from RA patients led to an alteration of OA-C1q which was now recognized by the anti-collagen type II antibody.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-02-15 | FEBS Letters |