Search results for "Typing"
showing 10 items of 1051 documents
Marinifilum flexuosum sp. nov., a new Bacteroidetes isolated from coastal Mediterranean Sea water and emended description of the genus Marinifilum Na…
2012
Abstract A facultatively anaerobe, moderately halophilic, Gram-negative, filamentous, non motile and unpigmented bacterium, designated M30 T , was isolated from coastal Mediterranean Sea water in Valencia, Spain. Phylogenetic analysis based on 16S rRNA sequences placed this strain in the phylum “ Bacteroidetes ” with Marinifilum fragile JC2469 T as its closest relative with 97% sequence similarity. Average nucleotide identity (ANI) values between both strains were far below the 95% threshold value for species delineation (about 89% using BLAST and about 90% using MUMmer). A comprehensive polyphasic study, including morphological, biochemical, physiological, chemotaxonomic and phylogenetic d…
Rapid prototyping for micro-engineering and microfluidic applications: Recycled pmma, a sustainable substrate material
2018
Poly(methylmethacrylate), PMMA, is one of the most commonly used thermoplastics for the manufacture of micromechanical and microfluidic devices, due to its optical transparency, rigid mechanical properties, low cost and good workability in conjunction with its rapid prototyping and mass manufacturing. Recent advances in the rapid-prototyping fields have allowed the production of precise features compatible with microfluidic structures and accelerated the conversion process from bench-side to mass market. For example, to address the need for fast design cycles using material compatible with mass manufacturing, we have developed an ultrafast prototyping technique for the manufacture of multil…
Genome-based analyses reveal a synonymy among Halorubrum distributum Zvyagintseva and Tarasov 1989; Oren and Ventosa 1996, Halorubrum terrestre Vento…
2020
A comparative taxonomic study of Halorubrum distributum , Halorubrum terrestre , Halorubrum arcis and Halorubrum litoreum was carried out using different approaches, 16S rRNA gene sequence analysis, multilocus sequence analysis (MLSA), phylogenomic analysis based on the comparison of the core genome, orthologous average nucleotide identity (OrthoANI), Genome-to-Genome Distance Calculator (GGDC), synteny plots and polar lipid profile (PLP). The MLSA study, using the five concatenated housekeeping genes atpB, EF-2, glnA, ppsA and rpoB′, and the phylogenomic analysis based on 1347 core translated gene sequences obtained from their genomes showed that Halorubrum distributum JCM 9100T, Halorubru…
Cluster of Legionnaires’ Disease in an Italian Prison
2019
Background: Legionella pneumophila (Lp) is the most common etiologic agent causing Legionnaires&rsquo
IL-23 receptor regulates unconventional IL-17-producing T cells that control bacterial infections.
2010
AbstractIL-23 plays an important role in autoimmune tissue inflammation and induces the generation of not fully characterized effector cells that mediate protection against pathogens. In this paper, we established the essential role of IL-23R in the host response against intracellular pathogens. IL-23 was critical for the expansion or maintenance of γδ and double negative (DN) αβ T cells. These cells were rapidly recruited to the site of infection and produced large amounts of IL-17, IFN-γ, and TNF-α. Notably, DN T cells transferred into L. monocytogenes-infected RAG2−/− mice prevented bacterial growth, confirming their protective role against intracellular pathogens. Our results show that …
Definition of the HLA-A2 restricted peptides recognized by human CD8+ effector T cells by flow-assisted sorting of the CD8+ CD45RA+ CD28– T cell subp…
2003
SUMMARY In response to antigenic stimulation, naive MHC-class I restricted and antigen-specific CD8+ CD45RA+ CD28+ T cells undergo clonal expansion, differentiate into CD8+ CD45RO+ memory T cells and convert to CD8+ CD45RA+ CD28− T cells displaying potent immune effector functions upon re-encounter with the nominal antigen. We show that the effector CD8+ CD45RA+ CD28– T cell subset is expanded in peripheral blood lymphocytes (PBL) from patients with human papilloma virus (HPV)+ cervical lesions as well as in PBL from patients with pulmonary tuberculosis. Flow-cytometric cell sorted CD8+ CD45RA+ CD28– and CD8+ CD45RA+ CD28– T cells were tested for recognition of HLA-A2 restricted peptides de…
Exploiting programmable architectures for WiFi/ZigBee inter-technology cooperation
2014
The increasing complexity of wireless standards has shown that protocols cannot be designed once for all possible deployments, especially when unpredictable and mutating interference situations are present due to the coexistence of heterogeneous technologies. As such, flexibility and (re)programmability of wireless devices is crucial in the emerging scenarios of technology proliferation and unpredictable interference conditions. In this paper, we focus on the possibility to improve coexistence performance of WiFi and ZigBee networks by exploiting novel programmable architectures of wireless devices able to support run-time modifications of medium access operations. Differently from software…
In Vitro Cultured Islet‐Derived Progenitor Cells of Human Origin Express Human Albumin in Severe Combined Immunodeficiency Mouse Liver In Vivo
2004
Studies in rodents suggest the presence of a hepatopancreatic stem cell in adult pancreas that may give rise to liver cells in vivo. The aim of the present study was to determine the ability of human islet-derived cells to adopt a hepatic phenotype in vivo. Cultured human islet-derived progenitor cells that did not express albumin in vitro were stained with the red fluorescent dye PKH26 and injected into the liver of severe combined immunodeficiency mice. After 3 or 12 weeks, red fluorescent cells were detected in 11 of 15 livers and were mostly single cells that were well integrated into the liver tissue. Human albumin was found in 8 of 11 animals by immunohistochemistry, and human albumin…
Splenic Marginal Zone Lymphoma Shows a Distinct Pattern of DNA Copy Number Aberrations That Correlates with Tumor Characteristics and Predicts Diseas…
2006
Abstract Splenic marginal zone lymphoma (SMZL) is an indolent B cell malignancy whose diagnosis is based on lymphocyte morphology, immunophenotype and marrow and/or splenic histology. Unlike other lymphomas, there is not a common chromosomal translocation specific for SMZL, and genetic prognostic factors are poorly defined. To investigate the pattern of genomic aberrations in SMZL, we applied comparative genomic hybridization to BAC microarrays (array CGH) to a well characterized series of 75 SMZL specimens. We applied two different 1 Mb-resolution BAC arrays: UCSF HumArray 3.2 and a novel array CGH platform developed at Univ. of Salamanca. These arrays allowed us to detect DNA copy number …
Pythium stipitatumsp. nov. isolated from soil and plant debris taken in France, Tunisia, Turkey, and India
2009
Pythium stipitatum is a slow-growing oomycete and has been isolated from soil samples and plant materials from France, Tunisia, Turkey and India. Its morphological characteristics are reminiscent of those of Pythium ramificatum, discovered in Algeria by the corresponding author. Unfortunately, the Algerian isolate was not deposited in any culture collection and ultimately got lost. Those were the days when molecular description of fungi was not a fashion; hence, no molecular characteristics of the Algerian isolates were deposited to the GenBank. Moreover, its coralloid antheridial branches made it an easy prey to be considered as synonymous to Pythium minus. Because there are no living stra…