Search results for "VIROME"
showing 10 items of 40 documents
A Method for Isolation of the Virome from Plasma Samples
2018
Virome studies are of special interest nowadays. Understanding viral communities in different body compartments will help guide future personalized treatments and to discern between homeostasis and disease. High-throughput sequencing technologies allow us to detect all the nucleic acids present in a sample, including viral ones, by random sequencing. One of the major challenges in virome studies is the correct isolation of the viral nucleic acids from a specific sample. This can be done during the extraction steps (e.g., enrichment of viral capsids), or during the bioinformatic analysis (e.g., removing all human and bacterial sequences). Furthermore, it is an important remark that the treat…
Diseño e implementación de una aplicación en Processing para la representación visual de datos multidimensionales utilizando técnicas de Minería de D…
2015
La posibilidad de disponer de representaciones gráficas de los datos es de gran valor a la hora de extraer conocimiento útil. Sus principales ventajas son la visualización de información de una forma sencilla, rápida y directa. No obstante, muchos de los conjuntos de datos contienen numerosos registros, que pueden ser de naturaleza multivariante. En estos casos, la representación visual de datos se convierte en una tarea complicada y las técnicas clásicas que suelen utilizarse obtienen resultados poco intuitivos. Esta tesis se plantea como objetivo el diseño e implementación de una aplicación versátil capaz de representar visualmente gran cantidad de datos multidimensionales de forma eficaz…
Metagenomic Analysis of Crohn's Disease Patients Identifies Changes in the Virome and Microbiome Related to Disease Status and Therapy, and Detects P…
2015
Background The aim of this study was to survey the bacterial and viral communities in different types of samples from patients with Crohn's disease (CD) at different stages of the disease to relate their distribution with the origin and progression of this disorder. Methods A total of 42 fecal samples and 15 biopsies from 20 patients with CD and 20 healthy control individuals were collected for bacterial 16S rRNA gene profiling and DNA/RNA virome metagenomic analysis through 454 pyrosequencing. Their composition, abundance, and diversity were analyzed, and comparisons of disease status, patient status, and sample origin were used to determine statistical differences between the groups. Resu…
Isolation in small populations of Wayampi Amerindians promotes endemicity and homogenisation of their faecal virome, but its distribution is not enti…
2018
The isolated community of the Wayampi Amerindians has been extensively studied for the presence of beta lactamase-producing enterobacteria and their gut microbiota. However, no information about their virome was available. This study tries to establish potential associations between the virome and diverse epidemiological data, through the metagenomic study of the faecal prophages and DNA viruses from 31 samples collected in 2010. Taxonomic assignments, composition, abundance and diversity analyses were obtained to characterise the virome and were compared between groups according to several demographic, environmental and medical data. Prophages outnumbered viruses. Composition and abundance…
Challenges in Energy Systems for the Smart-Cities of the Future
2012
In the last years the European Union has actively promoted the renewable energies and the Combined Heat and Power (CHP) also for residential and tertiary buildings. The exploitation of renewable sources and cogeneration seems hampered by the regulatory wall that prohibit for residential, tertiary and commercial buildings the constitution of users clusters. In fact, the unification up to a threshold value of some tens of kVA, at least, can facilitate the installation of renewable energy power plants as solar PV modules or CHP systems (cogeneration) or CCHP systems (tri-generation), overcoming technical and economical barriers and combining several load profiles. The actual distribution syste…
The sterilization of eco-criticism: from sustainable development to green capitalism
2015
[eng] Development has been a dominant and highly visible trope of the global political and economic life of the world since the 1950s. As such it has been inevitably linked to some of the most important social processes of this era: colonialism, globalization, postcolonialism, global ecological crisis, the rise of environmentalism, and more. The consolidation of the contemporary consumer global society came, hand by hand, with the certainty that it sustained a way of life that as a collateral damage, included a global ecological crisis. From many quarters of the world new voices raised concerns about the costs of globalization and proposed alternatives and solutions: modern eco-criticism wa…
The sterilization of eco-criticism: from sustainable development to green capitalism
2015
El desarrollo ha sido un tropo dominante de la vida política y económica global en el mundo desde 1950. Como tal ha estado inevitablemente conectado a algunos de los procesos sociales más importantes de esta era: colonialismo, globalización, post-colonialismo, crisis ecológica global, el crecimiento del ecologismo, entre otros. La consolidación de la sociedad del consumo contemporánea llegó de la mano de la certeza de que sostenía un sistema de vida que, como daño colateral, incluía una crisis ecológica global. Desde muchos lugares del mundo nuevas voces apuntaron críticas en relación a los costes de la globalización y propusieron alternativas y soluciones: el eco-criticismo. Este artículo …
El problema de la generación de residuos urbanos entre los contenidos de geografía de la enseñanza secundaria obligatoria
2002
Una de las características del problema de los Residuos Urbanos en nuestro país es la escasa conciencia ambiental de los ciudadanos. En este trabajo se exponen las grandes posibilidades que ofrece el currículo del Área de “Ciencias Sociales, Geografía e Historia” de la Enseñanza Secundaria Obligatoria (E.S.O.) para llevar a cabo acciones de Educación Ambiental, destacando así la capacidad de los profesores de Geografía para intentar mejorar la situación actual. Solid wastes generation problem into Secondary School Geography´s contents Citizenship’s low environmental conscience is a characteristic of Solid Wastes situation in Spain. In this paper, “Social Sciences, Geography and History” cur…
Vides aizsardzības kluba darbība 1987-1989
2021
Maģistra darba mērķis ir raksturot Vides Aizsardzības kluba laika periodā no 1987.–1989. gadam un izvērtēt organizācijas nozīmi Latvijas atmodas procesos. Aplūkoto laika periodu raksturo plašas politiskās izmaņas PSRS, kuru rezultāta sākas plašāka sabiedrības iesaiste politiskajos procesos. Pētījumā tiek sniegts ieskats organizācijas priekšvēsturē un izveidē, tās galvenajos darbības virzienos, problēmjautājumos, kuru risināšanā organizācija iesaistījās visplašāk, kā arī iemeslos, kādēļ 1989. gadā klubs nolēma kļūt par apolitisku organizāciju. Maģistra darbā izmantoti Latvijas valsts arhīvā un Zaļās Atmodas muzejā pieejamie materiāli par VAK darbību, tostarp organizācijas dibināšanas materiā…
Bias of library preparation for virome characterization in untreated and treated wastewaters
2020
The use of metagenomics for virome characterization and its implementation for wastewater analyses, including wastewater-based epidemiology, has increased in the last years. However, the lack of standardized methods can led to highly different results. The aim of this work was to analyze virome profiles in upstream and downstream wastewater samples collected from four wastewater treatment plants (WWTPs) using two different library preparation kits. Viral particles were enriched from wastewater concentrates using a filtration and nuclease digestion procedure prior to total nucleic acid (NA) extraction. Sequencing was performed using the ScriptSeq v2 RNA-Seq (LS) and the NEBNext Ultra II RNA …