Search results for "VITRO"
showing 10 items of 2786 documents
Differential requirements for antigen or homeostatic cytokines for proliferation and differentiation of human Vgamma9Vdelta2 naive, memory and effect…
2005
We have compared four human subsets of Vgamma9Vdelta2 T cells, naive (T(naive), CD45RA(+)CD27(+)), central memory (T(CM), CD45RA(-)CD27(+)), effector memory (T(EM), CD45RA(-)CD27(-)) and terminally differentiated (T(EMRA), CD45RA(+)CD27(-)), for their capacity to proliferate and differentiate in response to antigen or homeostatic cytokines. Cytokine responsiveness and IL-15R expression were low in T(naive) cells and progressively increased from T(CM) to T(EM) and T(EMRA) cells. In contrast, the capacity to expand in response to antigen or cytokine stimulation showed a reciprocal pattern and was associated with resistance to cell death and Bcl-2 expression. Whereas antigen-stimulated cells a…
Mast cell growth-enhancing activity (MEA) is structurally related and functionally identical to the novel mouse T cell growth factor P40/TCGFIII (int…
1990
We have previously shown that certain bone marrow-derived mast cell (BMMC) lines proliferate in response to a mast cell growth-enhancing activity (MEA) that is distinct from interleukin (IL) 3 and IL 4. Here we provide evidence that MEA is identical with the recently cloned mouse T cell growth factor P40. The evidence is as follows: (a) recombinant P40 displayed all the biological activities ascribed to MEA: it supported the growth of MEA-sensitive BMMC lines, it induced IL 6 secretion by these cells, and it enhanced survival of primary mast cell cultures; (b) highly purified MEA stimulated the growth of P40-dependent cell lines; (c) a rabbit monospecific antiserum directed against P40 spec…
Botāniskā dārza sekciju "Augu daudzveidības pētījumi ex situ", "Augu selekcija un introdukcija" tēžu krājums (2017. gada 9. februāris, LU Botāniskais…
2017
Latvijas Universitātes 75. konferences Augu daudzveidības pētījumi ex situ un Augu selekcijas un introdukcijas sekcijas sēdes notiek 2017. gada 9. februārī, plkst. 13.00 LU Botāniskajā dārzā Kandavas ielā 2, Rīgā.
Branched-Chain Amino Acid Deprivation Decreases Lipid Oxidation and Lipogenesis in C2C12 Myotubes
2022
Impaired lipid metabolism is a common risk factor underlying several metabolic diseases such as metabolic syndrome and type 2 diabetes. Branched-chain amino acids (BCAAs) that include valine, leucine and isoleucine have been proven to share a role in lipid metabolism and hence in maintaining metabolic health. We have previously introduced a hypothesis suggesting that BCAA degradation mechanistically connects to lipid oxidation and storage in skeletal muscle. To test our hypothesis, the present study examined the effects of BCAA deprivation and supplementation on lipid oxidation, lipogenesis and lipid droplet characteristics in murine C2C12 myotubes. In addition, the role of myotube contract…
Micropropagazione di Convolvulus sabatius Viv.
2015
Il genere Convolvulus, appartenente alla famiglia delle Convolvulaceae, comprende circa 250 specie annuali e perenni, molte delle quali sono considerate infestanti. Tuttavia, alcune specie come C. tricolor, C. cneorum e C. sabatius vengono inserite nel settore delle piante ornamentali (Bird, 2004). Specie erbacea perenne, originaria del bacino del mediterraneo, C. sabatius è rinvenibile nella nostra penisola allo stato spontaneo in Liguria, Puglia e Sicilia (Pignatti, 2003). La specie, per il valore estetico dei numerosi fiori azzurro-violetti, il lungo periodo di fioritura (aprile-giugno) e la capacità di adattamento sia ad alte che a basse temperature (la pianta tollera temperature fino a…
In vitro toxicity assessment and mitigation strategies of beauvericin, sterigmatocystin and patulin / Evaluación de la toxicidad in vitro y estrategi…
2016
Las micotoxinas son contaminantes químicos naturales presentes en los alimentos y piensos que representan un problema preferente económico y de salud pública. El conocimiento de los efectos tóxicos y exposición a las micotoxinas permite caracterizar los riesgos de la población. Se ha evaluado la toxicidad de la beauvericina, esterigmatocistina y patulina de forma individual y combinada mostrando efectos citotóxicos. Se ha observado que todas las micotoxinas produjeron citotoxicidad de manera individual al aumentar la concentración y el tiempo de exposición, siendo las células CHO-K1 más sensibles de mayor a menor potencia a la patulina, beauvericina y esterigmatocistina. Además, se observar…
Effect of the serum from multiple sclerosis patients on an in vitro model of blood-brain barrier.
2008
Multiple sclerosis (MS) is characterized by focal inflammatory demyelination, largely due to autoimmune responses against different components of the myelin sheet. It is also generally accepted that the pathogenesis of MS consists of inflammatory and neurodegenerative phases, where demyelination should produce partially reversible clinical deficits that can remit, due to limited remyelination, while axonal degeneration produces permanent non-remitting clinical damage. It is also assumed that nervous system inflammation is initiated by autoreactive, myelin-specific T cells that permeate the blood-brain barrier and trigger a series of events leading to tissue destruction. In addition to antib…
An approach to a biomimetic bone scaffold: increased expression of BMP-2 and of osteoprotegerin in SaOS-2 cells grown onto silica-biologized 3D print…
2012
Three-dimensional printed (3D printed) bone material is needed to close the shortage and to avoid the potential health risks associated with autografts and allografts, in the treatment of bone fractures/nonunions or bone trauma. Here we describe the fabrication of 3D printed scaffold, initially prepared form Ca-sulfate that has been impregnated/biologized with Ca-phosphate or with silica. The 3D printed grids had a size mesh of 200 μm; the chemical composition was determined by energy dispersive X-ray spectroscopy or conventional chemical analysis. Using human SaOS-2 cells (human osteogenic cells) it is shown that both the Ca-sulfate, and the Ca-phosphate or the silica impregnated Ca-sulfat…
The soluble terminal complement complex (SC5b-9) up-regulates osteoprotegerin expression and release by endothelial cells: Implications in rheumatoid…
2009
Objective. Complement activation products contribute to a large number of inflammatory diseases, including RA. We have investigated whether osteoprotegerin (OPG) may concur with the soluble terminal complement complex (SC5b-9) to the inflammatory cascade characterizing RA. Methods. Levels of SC5b-9 and OPG in the plasma and SF of patients with active RA were determined by ELISA. The presence of SC5b-9 and OPG in RA synovial lesions was analysed by immunohistochemistry. Cultured endothelial cells were used for in vitro leucocyte/endothelial cell adhesion assays. In addition, endothelial cells were exposed to SC5b-9 in order to evaluate the effects on the production of OPG protein, as well as…
Osteoprotegerin: multiple partners for multiple functions.
2013
Osteoprotegerin (OPG) is an essential secreted protein in bone turnover due to its role as a decoy receptor for the Receptor Activator of Nuclear Factor-kB ligand (RANKL) in the osteoclasts, thus inhibiting their differentiation. However, there are additional ligands of OPG that confer various biological functions. OPG can promote cell survival, cell proliferation and facilitates migration by binding TNF-related apoptosis inducing ligand (TRAIL), glycosaminoglycans or proteoglycans. A large number of in vitro, pre-clinical and clinical studies provide evidences of OPG involvement in vascular, bone, immune and tumor biology. This review describes an overview of the different OPG ligands regu…