Search results for "Western"

Cataract surgery in West Africa: is couching still a choice?

2012

MiR-221 promotes stemness of breast cancer cells by targeting DNMT3b

2016

// Giuseppina Roscigno 1, 2 , Cristina Quintavalle 1, 2 , Elvira Donnarumma 3 , Ilaria Puoti 1 , Angel Diaz-Lagares 4 , Margherita Iaboni 1 , Danilo Fiore 1 , Valentina Russo 1 , Matilde Todaro 5 , Giulia Romano 6 , Renato Thomas 7 , Giuseppina Cortino 7 , Miriam Gaggianesi 5 , Manel Esteller 4 , Carlo M. Croce 6 , Gerolama Condorelli 1, 2 1 Department of Molecular Medicine and Medical Biotechnology, “Federico II” University of Naples, Naples, Italy 2 IEOS-CNR, Naples, Italy 3 IRCCS-SDN, Naples, Italy 4 Epigenetic and Cancer Biology Program (PEBC) IDIBELL, Hospital Duran I Reynals, Barcelona, Spain 5 Department of Surgical and Oncological Sciences, Cellular and Molecular Pathophysiology Lab…

Type V collagen-induced upregulation of capn2 (large subunit of m-calpain) gene expression and DNA fragmentation in 8701-BC breast cancer cells

2011

Abstract Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts th…

The Beginning of Western Greek Amphorae Production in Western Sicily: Archaeometric and Archaeological Studies on 6th–5th Centuries BCE Amphorae Manu…

2020

About 560 western Greek amphorae (6th–5th centuries BCE) re-used in enchytrismos burials were unearthed in the necropolis of the Dorian-Chalcidian colony of Himera in northwestern Sicily. Among the most striking issues is the determination of their geographical provenance. For this purpose, ceramic samples chipped from freshly broken surfaces of all the amphorae were first subdivided into macrofabrics by the use of a hand lens. Thereon, the samples were studied in accordance with standardized methods by the use of reflected light microscopy. Due to the special focus of our project on the characterization of Sicilian productions, a selection of amphorae which showed visible, macroscopic affi…

Purification and Characterization of Glutamine Synthetase Isoenzymes from Leaves and Roots of Brassica napus (L.)

1995

Summary The glutamine synthetase enzymes from leaves (GS2) and roots (GSR) of Brassica napus L. have been purified to homogeneity by the application of a three-stage isolation procedure comprising anion-exchange chromatography, adsorption by hydroxylapatite and gel filtration on Sephacryl 5-300 HR. The isoforms of the enzyme show a differential distribution in leaf and root tissues. Elution profiles of hydroxylapatite chromatography showed a distinct behaviour for GS proteins found in leaves and roots. Denaturing SDS-PAGE and Western blot experiments revealed molecular masses of approximately 43.5 and 40.5 kD for GS2 and GSR subunits, respectively. Moreover, kinetic properties determined us…

Properties of sea urchin coelomocyte agglutinins

1996

We examined some biological activities of a 200-kDa glycoprotein, referred to as Paracentrotus lividus vitellogenin, contained both in the coelomic fluid and in a subpopulation of coelomocytes called «colourless spherula cells». Cell-free coelomic fluid, coelomocyte lysate and supernatant obtained after coelomocyte washings were assayed for hemagglutinating activity. All samples agglutinated rabbit erythrocytes in a calcium-dependent way. The comparison between the electrophoretic patterns of erythrocyte lysates, before and after incubation with the coelomic fluid, revealed that a 200-kDa band was obtained from membranes of agglutinated erythrocytes. In addition, polyclonal antibodies again…

The Roots of Chaadaev’s Philosophical Thought

2016

Pierwsza eurocentryczna koncepcja filozofii historii została stworzona przez Piotra Czaadajewa. Według tego chrześcijańskiego myśliciela jej ideał najlepiej odzwierciedlała średniowieczna Europa. Czaadajew czytał Kanta, Hegla i Schellinga, ale to francuscy filozofowie katoliccy: de Maistre, de Bonald, Ballanche, Chateaubriand i Lamennais, wywarli na niego największy wpływ. Specyficzny rodzaj konserwatywnego okcydentalizmu Czaadajewa charakteryzowało oskarżenie społeczeństwa rosyjskiego o brak tradycji, które mogłyby identyfikować Rosję z historią Europy. Według Czaadajewa kulturę rosyjską charakteryzuje brak ciągłości; nowe pomysły pojawiają się niespodziewanie, są pozbawione podstaw i brak…

S-type lectins occur also in invertebrates: high conservation of the carbohydrate recognition domain in the lectin genes from the marine sponge Geodi…

1993

The marine sponge Geodia cydonium contains several lectins. The main component, called lectin-1, is composed of three to four identical subunits. The subunits of the lectins were cloned from a cDNA library; two clones were obtained. From the deduced aa sequence of one clone, LECT-1, a mol. wt of 15,313 Da is calculated; this value is in good agreement with mass spectrometric analysis of 15,453 +/- 25 Da. The sequence of another clone, LECT-2, was analysed and the aa sequence was deduced (15,433 Da). The two subunits have a framework sequence of 38 conserved aa which are characteristic for the carbohydrate-binding site of vertebrate S-type lectins. Clustering of lectin sequences of various s…

CAMPANIAN WINE FOR PUNIC SICILY: PETROGRAPHIC AND ARCHAEOLOGICAL STUDIES OF GRAECO-ITALIC AMPHORAE FROM PALERMO

2018

This contribution proposes a combination of archaeological fabric analysis and petrographic research applied to the study of 35 sherds of Graeco-Italic amphorae mostly found in Palermo, but also in Pantelleria and Malta (Tab. 1). The provenance identification derived from both approaches gives evidence for the arrival, in North-Western Sicily, of presumable wine amphorae from central-Tyrrhenian Italy since the very late 4th century BC. The production of the majority of the material has been confidentially attributed to the area of the Gulf of Naples/Ischia, but a second large group originates from several, still unidentified production sites to be located along the coastal strip of Campania…

WRN protects against topo I but not topo II inhibitors by preventing DNA break formation

2008

The Werner syndrome helicase/3′-exonuclease (WRN) is a major component of the DNA repair and replication machinery. To analyze whether WRN is involved in the repair of topoisomerase-induced DNA damage we utilized U2-OS cells, in which WRN is stably down-regulated (wrn-kd), and the corresponding wild-type cells (wrn-wt). We show that cells not expressing WRN are hypersensitive to the toxic effect of the topoisomerase I inhibitor topotecan, but not to the topoisomerase II inhibitor etoposide. This was shown by mass survival assays, colony formation and induction of apoptosis. Upon topotecan treatment WRN deficient cells showed enhanced DNA replication inhibition and S-phase arrest, whereas af…