Search results for "Wolf"

Glucosylation of isatin-3-oxime followed by 2D in situ NMR in plant cells at highest magnetic field without labelling.

2001

The glucosylation of isatin-3-oxime (1) was monitored by in situ 2D 1H-13C inverse correlated gradient assisted NMR spectroscopy in plant cell suspension cultures of Rauvolfia serpentina without labelling. The applied high magnetic field of 800 MHz allowed measurements within 20 min at concentrations of 1 of 5.76 mM. Complete glucosylation of 1 occurs inside the cells within 72 hours. During this time isatin-3-oxime-glucoside (2) accumulates without further metabolism.

¿Qué hacer con el Mediterráneo?

2003

Purification, partial amino acid sequence and structure of the product of raucaffricine-O-β-d-glucosidase from plant cell cultures of Rauwolfia serpe…

1999

Plant cell suspension cultures of Rauwolfia produce within 1 week approximately 250 nkat/l of raucaffricine-O-beta-D-glucosidase. A five step procedure using anion exchange chromatography, chromatography on hydroxylapatite, gel filtration and FPLC-chromatography on Mono Q and Mono P delivered in a yield of 0.9% approximately 1200-fold enriched glucosidase. A short protocol employing DEAE sepharose, TSK 55 S gel chromatography and purification on Mono Q gave a 5% recovery of glucosidase which was 340-fold enriched. SDS-PAGE showed a Mr for the enzyme of 61 kDa. The enzyme is not glycosylated. Structural investigation of the enzyme product, vomilenine, demonstrated that the alkaloid exists in…

Los ritmos y la rima de la versificación goetheana en las versiones métricas del Fausto en español

2013

Los pocos traductores que han intentado hacer una traducción en verso del Fausto de Goethe no han logrado, en la mayoría de los casos, hacer justicia a la diversidad métrica de la obra del autor alemán. Algunos han abandonado por completo la idea, e.g. Pedro Gálvez en 1984, mientras que otros han creado un poema con métrica tradicional del español tomándose libertades excesivas en cuanto al contenido original del Fausto, tal como sucede en la versión de 1882 de Teodoro Llorente o en las de Valverde (1963) y Silvetti Paz (1970), quienes han empleado, en algunos casos, versos que parecen pentámetros yámbicos sin rima, una medida inusual en la poesía en español y que sin embargo resulta en oca…

Texte und Textschicksale in reformatorisch bewegter Zeit. Zum linguistischen Interesse an Texten des Greiffenberger Pastors Wolfgang Silbers des Jüng…

2016

After many years of search, the only surviving copy of a collection of sermons with a chronicle (965– 1618), which was printed and published in Leipzig in 1619 by Wolfgang Silber, the parson from Greiffenberg, Lower Silesia, was discovered in 2016, within the international programme “The Silesian Cultural Heritage.” The first part of the article includes information on the life and creation of the sermon’s author and presents the historical and cultural background of the book, with references to the municipality and the church. The history of Silber’s other texts is also related. The second part of the article focuses mainly on the historical and linguistic aspects of the Greiffenberg parso…

Nutritional evaluation of fresh and dried goji berries cultivated in Italy

2017

The nutritional profile of fresh and dried goji berries cultivated in Italy was investigated. The obtained data confirm goji berries as a source of nutritional and healthy components, such as vitamin E, minerals and fibre. Taking into account the Recommended Daily Allowance (RDA) for minerals and vitamins established by the Commission of the European Communities, Goji berries provide significant amounts of dietary fibre and zeaxanthin and can be declared on the label as a potential source of vitamins E and C. Moreover, dried goji berries can be declared as a source of K, P, Cu, Fe Mn, Zn.

3-Oxo-rhazinilam:  A New Indole Alkaloid from Rauvolfia serpentina × Rhazya stricta Hybrid Plant Cell Cultures

2000

A new monoterpenoid indole alkaloid, 3-oxo-rhazinilam (1), was isolated from intergeneric somatic hybrid cell cultures of Rauvolfia serpentina and Rhazya stricta, and the structure was determined by detailed 1D and 2D NMR analysis. It was also proved that 3-oxo-rhazinilam (1) is a natural constituent of the hybrid cells.

In vivo NMR at 800 MHz to monitor alkaloid metabolism in plant cell cultures without tracer labeling.

2001

Dynamics of the biosynthesis of methylursubin in plant cells employing in vivo 13CNMR without labelling.

1995

Abstract In vivo NMR experiments with a digital 600 MHz instrument, exploiting the natural abundance of 13C, allowed us for the first time to follow the biosynthesis of the newly detected glycoside, methylursubin ( 4- methoxyphenyl -O-β- d -primeveroside ), from 4-methoxyphenol through the intermediate methylarbutin in cell suspensions of the Indian medical plant, Rauwolfia serpentina. The metabolic dynamics indicate that, within 48 hr, 4-methoxyphenol is almost completely converted into the primeveroside, methylursubin. Because of the higher sensitivity at 150.9 MHz compared to that at 100.6 MHz, measuring times could be reduced to 1.5 hr. This allows detailed monitoring of the conversion …

Isolation and Structure Elucidation of a New Indole Alkaloid from Rauvolfia serpentina Hairy Root Culture: The First Naturally Occurring Alkaloid of …

2002

A new monoterpenoid indole alkaloid, 10-hydroxy- N(alpha)-demethyl-19,20-dehydroraumacline ( 1), was isolated as a mixture of E- and Z-isomers from hairy root culture of Rauvolfia serpentina Benth. ex Kurz (Apocynaceae) and the structure was determined by 1D and 2D NMR analyses. The new indole alkaloid represents the first naturally occurring alkaloid of the raumacline group and its putative biosynthetical pathway is discussed.