Search results for "analytical"

showing 10 items of 9586 documents

15-prostaglandin dehydrogenase expression alone or in combination with ACSM1 defines a subgroup of the apocrine molecular subtype of breast carcinoma.

2008

Established histopathological criteria divide invasive breast carcinomas into defined groups. Ductal of no specific type and lobular are the two major subtypes accounting for around 75 and 15% of all cases, respectively. The remaining 10% include rarer types such as tubular, cribriform, mucinous, papillary, medullary, metaplastic, and apocrine breast carcinomas. Molecular profiling technologies, on the other hand, subdivide breast tumors into five subtypes, basal-like, luminal A, luminal B, normal breast tissue-like, and ERBB2-positive, that have different prognostic characteristics. An additional subclass termed "molecular apocrine" has recently been described, but these lesions did not ex…

AdultSilver StainingBreast NeoplasmsBiologyProteomicsBiochemistrySubclassAnalytical ChemistryImmunophenotypingCohort StudiesBreast cancerCoenzyme A LigasesmedicineBiomarkers TumorHumansElectrophoresis Gel Two-DimensionalNeoplasm Invasivenessskin and connective tissue diseasesMolecular BiologyAgedAged 80 and overTissue microarrayParaffin EmbeddingApocrineMiddle Agedmedicine.diseaseImmunohistochemistryApocrine GlandsPhenotypeTissue Array AnalysisImmunologyCancer researchDisease ProgressionHydroxyprostaglandin DehydrogenasesImmunohistochemistryFemaleApocrine CellBreast carcinomaMolecularcellular proteomics : MCP
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Dietary exposure and risk assessment of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and dioxin-like polychlorinated biphenyls of…

2018

Dietary exposure of the Valencia Region population to polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and PCBs was assessed in the Region of Valencia in 2010–2011. A total of 7700 food samples were collected. Occurrence data were combined with consumption data to estimate dietary exposure in adults (>15 years of age) and young people (6–15 years of age). The estimated intake was calculated by a probabilistic approach. Average intake levels (upper-bound scenario) were 1.58 and 2.76 pg toxic equivalent (TEQ) kg−1 body weight (bw) day−1 for adults and young people, respectively. These average intakes are within range of the tolerable daily intake of 1–4 pg W…

AdultTolerable daily intakePolychlorinated DibenzodioxinsAdolescentHealth Toxicology and MutagenesisPopulationPolychlorinated dibenzodioxinsFood Contamination010501 environmental sciencesToxicologyRisk Assessment01 natural sciencesDietary ExposureYoung Adultchemistry.chemical_compoundAnimal scienceHumansMedicineChildeducationAged0105 earth and related environmental sciencesAged 80 and overeducation.field_of_studybusiness.industryDietary exposure010401 analytical chemistryPublic Health Environmental and Occupational HealthGeneral ChemistryGeneral MedicineDibenzofurans PolychlorinatedMiddle AgedPolychlorinated BiphenylsFood Analysis0104 chemical scienceschemistrySpainEnvironmental PollutantsbusinessRisk assessmentFood AnalysisPolychlorinated dibenzofuransFood ScienceFood contaminant
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Targeted Hybrid Nanocarriers as a System Enhancing the Skin Structure

2021

The skin is constantly exposed to external and internal factors that disturb its function. In this work, two nanosystems-levan nanoparticles and a surfactin-stabilized nanoemulsion were preserved (tested for microbial growth) and characterized (size, polydispersity, Zeta potential, and stability). The nanosystems were introduced in the model formulations-cream, tonic, and gel, and confirmed by TEM. The analysis showed that nanoemulsion has a spherical morphology and size 220–300 nm, while levan nanoparticles had irregular shapes independently of the use of matrix and with particle size (130–260 nm). Additionally, we examined the antiradical effect of levan nanoparticles and nanoemulsion in …

AdultskinMaterials scienceDPPHDispersitySkin CreamnanoemulsionPharmaceutical ScienceNanoparticleformulationMicrobial Sensitivity Tests02 engineering and technology030226 pharmacology & pharmacySkin DiscolorationsurfactinArticleAnalytical Chemistrylcsh:QD241-44103 medical and health scienceschemistry.chemical_compound0302 clinical medicinelcsh:Organic chemistryIn vivoDrug DiscoveryZeta potentialHumansPhysical and Theoretical ChemistryDrug CarriersBacteriaanti-agingOrganic ChemistryMiddle Aged021001 nanoscience & nanotechnologyDynamic Light ScatteringlevanFructansSkin AgingchemistryChemistry (miscellaneous)Molecular MedicineEmulsionsFemalenanoparticlesParticle sizeNanocarriers0210 nano-technologyBiomedical engineeringMolecules
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Screening of pharmacologic adulterant classes in herbal formulations using voltammetry of microparticles.

2013

A solid state electrochemical method for screening different families of adulterant chemicals illegally added to commercial phytotherapuetic formulations is described. The proposed method, based on the voltammetry of microparticles approach, permits a fast and sensitive way to distinguish between anorexics (amfepramone, fenproporex, sibutramine), benzozodiazepinic anxiolytics (clonazepam, flurazepam, alprazolam, midazolam, medazepam, chlordiazepoxide, diazepam), antidepressants (bupropione, fluoxetine, sertraline, paroxetine), diuretics (hydrochlorothiazide, furosemide, chlortalidone, amiloride, spironolactone), and hypoglycemics (glimepiride, chlorpropamide, glibenclamide) based on charact…

AdulterantChromatographyFlurazepamChemistryChemistry PharmaceuticalClinical BiochemistryAmfepramoneDrug Evaluation PreclinicalPharmaceutical ScienceElectrochemical TechniquesPharmacologyFenproporexMicrospheresAnalytical ChemistryMedazepamGlimepirideHydrochlorothiazideDrug DiscoverymedicinePlant PreparationsDrug ContaminationDiazepamSpectroscopymedicine.drugJournal of pharmaceutical and biomedical analysis
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Effective Henry's law partitioning and the salting constant of glyoxal in aerosols containing sulfate.

2013

The reversible partitioning of glyoxal was studied in simulation chamber experiments for the first time by time-resolved measurements of gas-phase and particle-phase concentrations in sulfate-containing aerosols. Two complementary methods for the measurement of glyoxal particle-phase concentrations are compared: (1) an offline method utilizing filter sampling of chamber aerosols followed by HPLC-MS/MS analysis and (2) positive matrix factorization (PMF) analysis of aerosol mass spectrometer (AMS) data. Ammonium sulfate (AS) and internally mixed ammonium sulfate/fulvic acid (AS/FA) seed aerosols both show an exponential increase of effective Henry's law coefficients (KH,eff) with AS concentr…

AerosolsAmmonium sulfateMolalityChromatography010504 meteorology & atmospheric sciencesSulfatesAnalytical chemistryGeneral ChemistryGlyoxal010501 environmental sciencesMass spectrometry01 natural sciencesHenry's lawAerosolchemistry.chemical_compoundchemistryIonic strengthTandem Mass SpectrometryEnvironmental ChemistryGlyoxalSaltsSulfateChromatography High Pressure Liquid0105 earth and related environmental sciencesEnvironmental sciencetechnology
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Drop-on-demand sample introduction system coupled with the flowing atmospheric-pressure afterglow for direct molecular analysis of complex liquid mic…

2012

One of the fastest developing fields in analytical spectrochemistry in recent years is ambient desorption/ionization mass spectrometry (ADI-MS). This burgeoning interest has been due to the demonstrated advantages of the method: simple mass spectra, little or no sample preparation, and applicability to samples in the solid, liquid, or gaseous state. One such ADI-MS source, the flowing atmospheric-pressure afterglow (FAPA), is capable of direct analysis of solids just by aiming the source at the solid surface and sampling the produced ions into a mass spectrometer. However, direct introduction of significant volumes of liquid samples into this source has not been possible, as solvent loads c…

AerosolsAtmospheric pressureChemistryDrop (liquid)Analytical chemistryAnalytic Sample Preparation MethodsAnalytic Sample Preparation MethodsReproducibility of ResultsMass spectrometryArticleAnalytical ChemistryAfterglowSpectrochemistryAtmospheric PressureLimit of DetectionCalibrationMass spectrumMicrotechnologySample preparationAnalytical chemistry
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Towards comprehensive non-target screening using heart-cut two-dimensional liquid chromatography for the analysis of organic atmospheric tracers in i…

2021

Abstract Non-target screening of secondary organic aerosol compounds in ice cores is used to reconstruct atmospheric conditions and sources and is a valuable tool to elucidate the chemical profiles of samples with the aim to obtain as much information as possible from one mass spectrometric measurement. The coupling of mass spectrometry to chromatography limits the results of a non-target screening to signals of compounds within a certain polarity range based on the utilized stationary phases of the columns. Comprehensive two-dimensional liquid chromatography (LCxLC) introduces a second column of different functionality to enable the analysis of a broader range of analytes. Conventional LCx…

AerosolsDetection limitChromatography Reverse-PhaseAnalyteChromatographyChemistryHydrophilic interaction chromatographyOrganic ChemistryGeneral MedicineRepeatabilityMass spectrometrySnowBiochemistryMass SpectrometryAnalytical ChemistryAerosolVolume (thermodynamics)Hydrophobic and Hydrophilic InteractionsChromatography LiquidJournal of Chromatography A
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Gel Electrophoresis Coupled to Inductively Coupled Plasma−Mass Spectrometry Using Species-Specific Isotope Dilution for Iodide and Iodate Determinati…

2007

In this paper, we present an online coupling of gel electrophoresis (GE) and inductively coupled plasma-mass spectrometry (ICP-MS) for the determination of iodine species (iodide and iodate) in liquid (seawater) and aerosol samples. For the first time, this approach is applied to the analysis of small molecules, and initial systematic investigations revealed that the migration behavior as well as the detection sensitivity strongly depends on the matrix (e.g., high concentrations of chloride). These effects could consequently affect the accuracy of analytical results, so that they need to be considered for the analysis of real samples. The technique used for quantification is species-specifi…

AerosolsElectrophoresis Agar Gelchemistry.chemical_classificationTime FactorsIodideIodatesAnalytical chemistrychemistry.chemical_elementIodidesIsotope dilutionIodineMass spectrometrySensitivity and SpecificityMass SpectrometryAnalytical Chemistrychemistry.chemical_compoundIsotopesSpecies SpecificitychemistrySample preparationTriiodideInductively coupled plasma mass spectrometryIodateAnalytical Chemistry
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Real-Time Analysis of Ambient Organic Aerosols Using Aerosol Flowing Atmospheric-Pressure Afterglow Mass Spectrometry (AeroFAPA-MS).

2015

Organic compounds contribute to a major fraction of atmospheric aerosols and have significant impacts on climate and human health. However, because of their chemical complexity, their measurement remains a major challenge for analytical instrumentation. Here we present the development and characterization of a new soft ionization technique that allows mass spectrometric real-time detection of organic compounds in aerosols. The aerosol flowing atmospheric-pressure afterglow (AeroFAPA) ion source is based on a helium glow discharge plasma, which generates excited helium species and primary reagent ions. Ionization of the analytes occurs in the afterglow region after thermal desorption and pro…

AerosolsIonsAtmospheric pressureChemistryAnalytical chemistryGeneral Chemistryrespiratory systemMass spectrometryIon sourceMass SpectrometryAerosolAfterglowIonAtmospheric PressureComputer SystemsIonizationMass spectrumEnvironmental ChemistryHumansOrganic ChemicalsEnvironmental sciencetechnology
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Identification of organic hydroperoxides and hydroperoxy acids in secondary organic aerosol formed during the ozonolysis of different monoterpenes an…

2009

On-line ion trap mass spectrometry (ITMS) enables the real-time characterization of reaction products of secondary organic aerosol (SOA). The analysis was conducted by directly introducing the aerosol particles into the ion source. Positive-ion chemical ionization at atmospheric pressure (APCI(+)) ITMS was used for the characterization of constituents of biogenic SOA produced in reaction-chamber experiments. APCI in the positive-ion mode usually enables the detection of [M+H](+) ions of the individual SOA components. In this paper the identification of organic peroxides from biogenic volatile organic compounds (VOCs) by on-line APCI-ITMS is presented. Organic peroxides containing a hydroper…

AerosolsOrganic peroxideChemical ionizationAir PollutantsSpectrometry Mass Electrospray IonizationOzonolysisAtmospheric pressureOrganic ChemistryAnalytical chemistryCarboxylic AcidsAtmospheric-pressure chemical ionizationHydrogen PeroxideOnline SystemsIon sourceAnalytical ChemistryPeroxideschemistry.chemical_compoundchemistryFunctional groupMonoterpenesPressureVolatilizationHydrogen peroxideSesquiterpenesSpectroscopyRapid communications in mass spectrometry : RCM
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